بررسی فعالیت آنتی‌اکسیدانی جلبک‌ دریایی قرمز Champia compressa سواحل چابهار

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، ایران.

2 نویسنده مسئول، دانشیار فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، ایران.

چکیده

جلبک های دریایی دارای خواص آنتی اکسیدانی هستند. در پژوهش حاضر، فعالیت آنتی‌اکسیدانی عصاره های آلی جلبک‌ قرمز Champia compressa خلیج چابهار مورد آزمون قرار گرفت. استخراج عصاره‌ از نمونه‌ها با غلظت 1:20 (حلال به جلبک) به روش خیساندن به مدت 24 ساعت در دمای محیط انجام شد. حلال‌های مورد استفاده شامل متانول، هگزان، دی‌کلرومتان و کلروفرم در سطوح 01/0 ،1/0، 5/0 و 1 میلی گرم بر میلی لیتر بود. از آزمون‌های حذف رادیکال آزاد DPPH، احیاکنندگی و چلاته کنندگی یون آهن استفاده شد. حداقل مقدار IC50 فعالیت مهار رادیکال آزاد DPPH جلبک compressa C. در عصاره متانولی با مقدار 05/1 میلی‌گرم بر میلی‌لیتر و حداکثر مقدار آن در دی‌کلرومتان محاسبه شد. همچنین، حلال کلروفرم با IC50 64/2 بیشترین میزان کلاته‌کنندگی را داشت. عصاره متانولی با IC50 68/0 میلی گرم در میلی لیتر بیشترین فعالیت کاهندگی را داشته است. همچنین، با افزایش غلظت عصاره، میزان فعالیت آنتی‌اکسیدانی آن نیز افزایش می-یابد. به طوری‌که، مهار رادیکال آزاد DPPH، قدرت کلاته کنندگی و فعالیت کاهندگی در غلظت 1 میلی گرم بر میلی‌لیتر اکثر عصاره‌ها نسبت به سایر غلظت‌ها اختلاف معنی داری را نشان داده است (p<0.05). در مجموع می‌توان نتیجه گرفت که عصاره متانولی و کلروفرمی جلبک compressa C. دارای قدرت آنتی‌اکسیدانی خوبی می‌باشد. تحقیقات بیشتر برای بررسی کامل تر خواص آنتی اکسیدانی عصاره های این جلبک توصیه می شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study the antioxidant activity of marine red algae Champia compressa from Chabahar coasts

نویسندگان [English]

  • Sedigh Mohammadi 1
  • ALI Taheri 2
1 M.Sc. Graduate of Fish Processing Technology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
2 Corresponding Author, Associate Prof., Dept. of Fish Processing Technology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
چکیده [English]

Seaweeds have antioxidant properties. In this study, antioxidant activity of red algae Champia compressa organic extracts from Chabahar Bay was investigated. Extraction of the samples was done with a concentration of 1:20 (solvent to algae) by soaking the algae powder for 24 hours at ambient temperature. The solvents included methanol, hexane, dichloromethane and, chloroform at concentrations of 0.01, 0.1, 0.5 and 1, mg/mL. Extracts were subjected to the renowned antioxidant activity assay procedures of DPPH free radical scavenging activity, reduction power and, iron chelating. The minimum IC50 value of DPPH free radical scavenging activity of C. compressa algae was in the methanolic extract with a value of 1.05 mg/mL. The maximum value was calculated in dichloromethane extract. Also, chloroform solvent with IC50 of 2.64 had the highest chelation. Methanolic extract with IC50 of 0.68 mg/mL had the highest reducing activity. with the increase in the concentration of the extract, the amount of its antioxidant activity increased. So, inhibition of DPPH free radical, chelating power and, reducing activity at a concentration of 1 mg/mL of most extracts showed a significant difference compared to other concentrations (p<0.05). In conclusion, the methanolic and chloroform extracts of C. compressa red algae exhibited notable antioxidant properties. Further research for a complete investigation of the antioxidant properties of the C. compressa extracts is need.

کلیدواژه‌ها [English]

  • Champia compressa
  • Antioxidant
  • Chabahar
  • Free Radical
  • Chelating
1.Paktarmani, M., Ehsani, A., & Qajarbeygi, P. (2017). Investigating -Increase the shelf life of fish with edible alginate sodium-Based film containing α-tocopherol. Journal of Food Science and Technology, 13 (61), 17-24.2.Shahidi, F., & Zhong, Y. (2008). Bioactive Peptides. Journal of AOAC International, 91, 914-931.3.Gordon, M. H. (1996). The mechanism of antioxidant action in vitro. In: Food Antioxidants. Hudson, B. J. F. (ed). Pp 1-18. Elsevier Applied Science, London, UK.4.Roginsky, V., & Lissi, E. (2005). Review of methods to determine chain-breaking antioxidant activity in food. Food Chemistry, 92, 235-254.5.Kuda, T., Tsunekawa, M., Goto, H., & Araki, Y. (2005). Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan. Journal of Food Composition and Analysis, 18 (7), 625-633.6.Gupta, S., & Abu-Ghannam, N. (2011). Bioactive potential and possible health effects of edible brown seaweeds. Trends in Food Science & Technology, 22 (6), 315-326.7.Vladkova, T., Georgieva, N., Staneva, A., & Gospodinova, D. (2022). Recent Progress in Antioxidant Active Substances from Marine Biota. Antioxidants, 11 (3), 439. 8.Babakhani Lashkan, A., Rezaei, M., Rezaei, K., & Seifabadi, S. J. (2012). Optimization of extraction of antioxidant compounds in microwave-assisted extracts of brown algae Sargassum angustifolium. Journal of Fisheries, 65 (3), 243-255.9.Taheri, A. (2016). Antioxidant activity in some Iranian seaweed species from Chabahar. Iranian Journal of Fisheries Sciences, 15 (2), 802-817.10.Taheri, A., Ghaffari, M., Bagherpour, N. S., & Attaran Fariman, G. (2017). Study the Antioxiative Properties of the Marine Algae Cystoseira trinodis extracts from Chabahar Coastal Water. Journal of Shahid Sadoughi University, 25 (8), 658-669.11.Taheri, A., Ghaffari, M., Bagherpour, N., & Attaran Fariman, G. (2017). Evaluation of Antioxidant Activity of Extracts of Marine Algae Halimeda tuna Collected from the Chabahar Bay. Qom University of Medical Sciences Journal, 11 (5), 107-115.12.Gahramzei, M., & Taheri, A. (2021). Antioxidant Properties of Organic Extracts of Seaweed Rhizoclonium riparium from Oman Sea. Journal of Marine Medicine, 3 (2), 107-115.13.Aghajanpoor, N., Babakhani, A., & Tabarsa, M. (2020). Optimization of the extraction of antioxidant compounds from brown algae Sargassum angustifolium in the Persian Gulf by response surface methodology (RSM). Aquaculture Sciences, 8 (1), 43-58.14.Ahadifar, M., Ojagh, S. M., Hoseinifar, H., Kordjazi, M., Khanlar, M., & Alishahi, A. (2021). Evaluation of antioxidant properties of aqueousextract of brown Sargassum vulgare macroalgae collected from Qeshm coast. Utilization and Cultivation of Aquatics, 10 (3), 49-63.15.Gharanjik, B. M., & Rohani Ghadikolaei, K. (2009). Atlas of the sea algae of the Persian Gulf and Oman Sea coasts. Iranian Fisheries Research Organization, Tehran. 180p.16.Harvey, W. H. (1838). The genera of South African plants, arranged according to the natural system. 429 p. Cape Town: A. S. Robertson, 21, Heeregracht.17.Park, M. R., & Lee, I. K. (1998). Morphology and Phenology of Champia expansa Yendo and C. compressa Harvey from Korea. Algae, 13 (1), 85-99.18.Brand-Williams, W., Cuvelier, M. E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT- Food Science and Technology, 28, 25-30.19.Dinis, T. C. P., Madeira, V. M. C., & Almeida, L. M. (1994). Action of phenolic derivates (acetoaminophen, salycilate and 5- aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archive of Biochemistry and Biophysics, 315 (1), 161-169.20.Oyaizu, M. (1986). Studies on products of browning reaction. The Japanese Journal of Nutrition and Dietetics,
44 (6), 307-315.21.Ak, I., & Turker, G. (2018). Antioxidant Activity of Five Seaweed Extracts.
New Knowledge Journal of Science, 7 (2), 149-155.22.Al-Amro, A. A., Al-Mutlaq, M. A.,
Al-Moauther, S., Al-Tukhaifi, N., Othaimeen, R. B., Al-Mutairi, N.,Al-Rashed, S., Al-Malki, F., & Hyder Haq, S. (2019). Antioxidant Activity of Rhodophyta Algae Polysiphonia and Laurencia Collected from the Arabian Gulf. Asian Journal of Applied Sciences, 12, 71-75.23.Heidari, M., Zolgharnine, H., Sakhaei, N., Mirzaei, A., & Movahedinia, A. (2015). Antibacterial and Anti-oxidant activity of three species of green, brown and red algae from Northern coast of Persian Gulf. Iran South Medical Journal, 18 (2), 383-392.24.Heidari, M., Movahedinia, A., & Hosseini, S. (2017). Assessment of antiradical and antioxidant potentials in two red and brown algae from Persian Gulf in Booshehr province in comparison with leaf of mangrove (Avicennia marina). Journal of Environmental Science and Technology, 19 (5), 179-189.25.Hidayati, J. R., Yudiati, E., Pringgenies, D., Oktaviyanti, D. T., & Kusuma, A. P. (2020). Comparative study on antioxidant activities, total phenolic compound and pigment contents of tropical Spirulina platensis, Gracilaria arcuata andUlva lactuca extracted in different solvents polarity. The 3rd International Symposium on Marine and Fisheries Research, Indonesia, 147, 1-9 (03012).26.Kumar, A., & Chattopadhyay, S. (2007). DNA damage protecting activity and antioxidant potential of pudina extract. Food Chemistry, 100, 1377-1384.27.Rajauria, G., Jaiswal, A. K., Gannam, M. A., & Gupta, S. (2013). Antimicrobial, Antioxidant and free radical – Scavenging capacity of brown seaweed Himanthalia elongate from western coast of Irel. Journal of Food Biochemistry, 37 (3), 322-335.28.Lee, N. Y., Yunus, M. A. C., Idham, Z., Ruslan, M. S. H., Aziz, A. H. A., & Irwansyah, N. (2017). Extraction and identification of bioactive compounds from agarwood leaves. Second International Conference on Chemical Engineering (ICCE), 162, 1-6.29.Mohammadi, E., Shabanpourh, B., & Kordjazi, M. (2017). Investigation of physicochemical properties, fattyacid profile and sensory evaluation of Iyengaria stellata, Journal ofAquatic Physiology and Biotechnology, 5 (3), 25-38.30.Mohammadi, E., Shabanpourh, B., & Kordjazi, M. (2019). Effect of Nizimuddinia zanardini aqueous extract against human pathogenic microbes and evaluating its antioxidant activity. Journal of Marine Biology, 10 (4), 75-82.31.Louis, P., Arive, C., Inquimboy, H., & Lazaro-Llanos, N. (2017). In Vitro Antioxidant Activity of Selected Seaweeds in the Philippines. International Journal of Theoretical & Applied Sciences, 9 (2), 212-216.32.Ebrahimzadeh, M. A., Khalili, M., & Dehpour, A. A. (2018). Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis an in vitro assay. Brazilian Journal of Pharmaceutical Sciences, 54 (1), 17280.33.Chia, Y. Y., Kanthimathi, M. S., Khoo, K. S., Rajarajeswaran, J., Cheng, H. M., & Yap, W. S. (2015). Antioxidant and cytotoxic activities of three species of tropical seaweeds. BMC Complement and Alternative Medicine, 15, 339.34.Adham, E. K. A., Hassan, A. I., Hamed, S. M., & Saber, A.A. (2017). Evaluation of iron-chelating activity of Caulerpa racemosa in iron-dextran induced iron overload in an experimental model of thalassemia. International Journal of Clinical and Experimental Medicine, 10 (3), 5561-5577. 35.Mekinic, I. V., Skroza, D., Simat, V., Hamed, H., Cagalj, M., & Perkovi, Z. P. (2019). Phenolic Content of Brown Algae (Pheophyceae) Species: Extraction, Identification, and Quantification. Biomolecules, 9, 244.36.Sanjeewa, K. K. A., Jayawardena, T. U., Kim, H. S., Kim, S. Y., Ahn, G., Kim, H. J., Fu, X., Jee, Y., & Jeon, Y. J. (2019). Ethanol extract separated from Sargassum horneri (Turner) abate
LPS-induced inflammation in RAW 264.7 macrophages. Fish and Aquatic Sciences, 22 (6), 1-10.37.Kim, J. J., Kim, Y. S., & Kumar, V. (2019). Heavy metal toxicity: An update of chelating therapeutic strategies. Journal of Trace Elements Medical Biology, 54, 226-231.38.Corsetto, P. A., Montorfano, G., Zava, S., Colombo, I., Ingadottir, B., Jonsdottir, R., Sveinsdottir, K., &
Rizzo, A. (2020). Characterization of Antioxidant Potential of Seaweed Extracts for Enrichment of Convenience Food. Antioxidants, 9, 249. 39.Gontijo, V. S., Souza, T. C., Rosa, I. A., Soares, M. G., Silva, M. A., Vilegas, W., Viegas, C., & Santos, M. H. (2012). Isolation and evaluation of the antioxidant activity of phenolic constituents of the Garcinia brasiliensis epicarp. Food Chemistry, 132 (3), 1230-1235.40.Sharma, P., Gujral, H. S., & Singh, B. (2012). Antioxidant activity of barley as affected by extrusion cooking. Food Chemistry, 131 (4), 1406-1413.41.Shanmuga Rajan, N., & Bhat, R. (2016). Antioxidant compounds and antioxidant activities in unripe and ripe kundang fruits (Bouea macrophylla Griffith). Fruits, 71 (1), 41-47.42.Alam Sobuj, M. K., Ariful Islam, M., Shoebul Islam, M., Mohidul Islam, M., Mahmud, Y., & Rafiquzzaman, S. M. (2021). Effect of solvents on bioactive compounds and antioxidant activity of Padina tetrastromatica and Gracilaria tenuistipitata seaweeds collected from Bangladesh. Scientifc Reports,11, 19082.43.Bhuyar, P., Rahim, M. H., Sundararaju, S., Maniam, G. P., & Govindan, N. (2020). Antioxidant and antibacterial activity of red seaweed Kappaphycus alvarezii against pathogenic bacteria. Global Journal of Environmental Science and Management, 6 (1), 47-58.44.Taheri, A., Ghaffari, M., Houshmandi, S., & Namavari, M. M. (2017). Investigation of the anticancer and antioxidant activity of the brownalgae (Cystoseira indica) extract against the colorectal cancer cells. Feyz, 21 (4), 317-325.45.Blanc, N., Hauchard, D., Audibert, L., & Ar Gall, E. (2011). Radical-scavenging capacity of phenol fractions in the brown seaweed Ascophyllum nodosum. An electrochemical approach. Talanta, 84, 518-513.46.Cai, Y. Z., Sun, M., Xing, J., Luo, Q., & Corke, H. (2006). Structure-radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life Science, 78, 2872-2888. 47.Kumaran, A., & Karunakaran, R. J. (2007). Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry, 97, 109-114.