اثرات تغذیه‌ای پلی‌فنل گیاه پیچک (Hedera helix) بر بیان ژنهای انتی اکسیدانی و پارامترهای بیوشیمیایی ماهی زبرا (Danio rerio) در مواجهه با نانوپلاستیک پلی‌اتیلن

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری شیلات، گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 نویسنده مسئول، دانشیار گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 دانشیار گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

4 استاد گروه تولید و بهره‌برداری آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

5 استاد گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

6 استاد آناتومی و سیتولوژی تطبیقی، گروه علم و فناوری، دانشگاه سانیو، بنونتو، ایتالیا.

چکیده

آلودگی ناشی از نانوپلاستیک‌ها تهدیدی جدی برای اکوسیستم‌ها و سلامت آبزیان است. این مطالعه با هدف بررسی اثرات مکمل پلی‌فنل‌ استخراج‌شده از گیاه پیچک (Hedera helix) در کاهش آسیب‌های ناشی از نانوپلاستیک‌ها در ماهی زبرا (Danio rerio) پرداخت. در این راستا، آزمایشی در دو مرحله طراحی شد. در مرحله اول ماهی‌های زبرا به مدت 8 هفته با مکمل پلی‌فنل در سطوح صفر، 0/5، 1 و 2 درصد تغذیه شدند. در مرحله دوم آزمایش، تیمار‌های مذکور به مدت 2 هفته در مواجهه با غلظت ثابت 1 درصد نانوپلاستیک پلی‌اتیلن نگهداری و با جیره‌های مشابه تغذیه شدند. در پایان هر مرحله نمونه برداری جهت بررسی شاخص‌های بیوشیمیایی ALT، ALP، AST، CAT و SOD و همچنین بررسی بیان نسبی ژن‌های SOD و CAT انجام شد. به طور کلی، نتایج به دست آمده نشان دادند در هر مرحله از آزمایش سطح شاخص‌های بیوشیمیایی ALT، ALP، AST، CAT و SOD به شکل مستقیم وابسته به غلظت مکمل پلی‌فنل بودند و در مقایسه با گروه شاهد افزایش معنادار نشان دادند (05/0>p). بررسی بیان نسبی ژن‌های SOD و CAT نشان داد استفاده از مکمل پلی‌فنل به تنهای یا در مواجهه با نانوپلاستیک منجر به افزایش سطح بیان این ژن‌ها می‌گردد (05/0>p). این تحقیق نشان می‌دهد که پلی‌فنل‌های گیاه پیچک می‌توانند به‌عنوان یک راهکار مؤثر برای کاهش آسیب‌های اکسیداتیو ناشی از نانوپلاستیک‌ها و بهبود سلامت آبزیان عمل کنند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of dietary supplementation of Hedera helix polyphenol antioxidant gene expression and biochemical parameters of zebrafish ( Danio rerio) exposed to polyethylene nanoplastic

نویسندگان [English]

  • Farahnaz Kakavand 1
  • Roqayeh Safari 2
  • Seyed Hossein Hoseinifar 3
  • Seyed Aliakbar Hedayati 4
  • Mohammadreza Imanpor 5
  • Marina Paolucci 6
1 Ph.D. Student in Fisheries, Dept. of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Corresponding Author, Associate Prof., Dept. of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Associate Prof., Dept. of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Professor, Dept. of Aquatics Production and Exploitation, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
5 Professor, Dept. of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
6 Professor of Comparative Anatomy and Cytology, Dept. of Science and Technology, University of Sannio, Benevento, Italy.
چکیده [English]

Nanoplastic pollution poses a significant threat to ecosystems and aquatic organisms' health. This study aimed to investigate the protective effects of polyphenols extracted from ivy (Hedera helix) against the damages caused by nanoplastics in Zebrafish (Danio rerio). A two-phase experiment was designed. In the first phase, zebrafish were fed with diets supplemented with polyphenols at levels of 0%, 0.5%, 1%, and 2% for 8 weeks. In the second phase, the treatment groups were exposed to a constant concentration of 1% polyethylene nanoplastics for another 2 weeks while continuing with the same diets. At the end of each phase, samples were collected to assess biochemical parameters such as ALT, ALP, AST, CAT and SOD, as well as the relative expression of SOD and CAT genes. Overall, the results showed that the levels of ALT, ALP, AST, CAT and SOD increased significantly (p<0.05) with increasing polyphenol supplementation in both phases of the experiment. The relative expression of SOD and CAT genes also increased significantly (p<0.05) when polyphenols were supplemented alone or in combination with nanoplastics. This research suggests that ivy polyphenols can be a promising strategy to mitigate oxidative damage caused by nanoplastics and improve the health of aquatic organisms.

کلیدواژه‌ها [English]

  • Antioxidant
  • Pollution
  • Whole body extract
  • Zebrafish

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مجله بهره برداری و پرورش آبزیان
دوره 14، شماره 3
مهر 1404
صفحه 153-166

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