اثرات به‌کارگیری پکتین و مولتی آنزیم ناتوزیم به صورت مجزا و ترکیبی بر فاکتورهای بیوشیمیایی خون در ماهی کپور معمولی (Cyprinus carpio)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 نویسنده مسئول، دانشجوی دکتری تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

2 گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط‌زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

چکیده

آنزیم‌ها و پکتین ترکیبی از مولکولهای مختلفی هستند که نقش اصلی را در سامت و رشد ابزیان دارد. در این آزمایش اثر به‌کارگیری پکتین و مولتی آنزیم ناتوزیم به صورت مجزا و توام بر شاخص‌های بیوشیمیایی سرم خون در بچه ماهی کپور معمولی مورد بررسی قرار گرفت. بدین‌منظور تعداد 540 قطعه بچه ماهی کپورمعمولی با میانگین وزن حدود 1±33/ 16گرم با جیره‌های آزمایشی با نه تیمار شامل جیره‌ی پایه (صفر)، 1 درصد پکتین ، 2 درصد پکتین ، 05/0 درصد مولتی آنزیم ناتوزیم، 1/0 درصد مولتی آنزیم ناتوزیم ،تلفیق پکتین(1 درصد) و مولتی آنزیم ناتوزیم (05/0 درصد)،تلفیق پکتین (2درصد) و مولتی آنزیم ناتوزیم(05/0 درصد)، تتلفیق پکتین (1درصد) و مولتی آنزیم ناتوزیم (1/0 درصد) ،تلفیق پکتین (2درصد) و مولتی آنزیم ناتوزیم (1/0 درصد) به مدت 8 هفته تغذیه شدند. در انتهای دوره فاکتورهای بیوشیمیایی خون پروتئین کل، آلبومین، گلوبولین و آنزیم‌های کبدی (ALT,AST,ALP)، از روش اسپکتوفتومتری اندازه گیری شدند. .نتایج نشان داد که میزان آلکالین فسفاتاز، آسپارتات آمینوترانسفراز و آلانین آمینوترانسفراز در هیچ یک از تیمارهای مجزای پکتین و مولتی آنزیم ناتوزیم و تیمار تلفیقی (با اثر متقابل) نشان نداد. . نتایج بررسی پروتئین، آلبومین و ایمنوگلوبین سرم خون ماهیان گروه شاهد و ماهیان تغذیه شده با مولتی آنزیم ناتوزیم 05/0 و 1/0 درصد اختلاف معنی داری را نشان نداد اما با تیمارهای حاوی پکتین و تیمارهای ترکیبی اختلاف معنی داری مشاهده شد(05/0P≤). به طور کلی به-کارگیری پکتین و مولتی آنزیم ناتوزیم به صورت مجزا و توام باعث بهبود فاکتورهای بیوشیمایی خون به عنوان شاخص های ایمنی در ماهی کپور گردید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effects of using Pectin and Natozyme multi-enzyme separately and in combination on blood biochemical factors in Common Carp (Cyprinus carpio)

نویسندگان [English]

  • Tahereh Darnahal 1
  • Mohamadreza Imanpour 2
  • Roghieh Safari 3
1 Corresponding Author, Ph.D. Student of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

Enzymes and pectin are composed of variety of molecules which play the major role in the improvement of growth performance and health of aquatic animals. In this experiment, the effect of using pectin and Natozyme eparately and in combination on blood biochemical indices in common carp fry (Cyprinus carpio) was investigated. For this purpose, the number of 540 common carp fry with an average weight of 16.33±1 g were fed with nine treatments including basic diet (0), 1% pectin, 2% pectin 0.05% natozyme, 0.1% Natozyme, combination of pectin (1%) and Natozyme (0.05%), pectin (2%) and Natozyme (0.05%), Pectin (1%) and Natozyme (0.1%), pectin composition (2%) Natozyme (0.1%) for 8 weeks. At the end of the expriment, blood biochemical factors, total protein, albumin, globulin and liver enzymes (ALT, AST, ALP) were measured by spectrophotometric method. The amount of ALT, AST, ALP did not show significant difference in separate treatments of pectin and Natozyme and the combined treatment (P>0.05). The results of protein, albumin, and serum immunoglobulin in the control group and fish fed with 0.05 and 0.1% Natozyme showed no significant difference (P≥0.05) but significant differences with those fed diet including pectin and combination of pectin and Natozyme (P≤0.05). In general, the use of pectin and Natozyme separately and in combination improved blood biochemical factors as imminity indicators in carp fish.

کلیدواژه‌ها [English]

  • natozyme
  • pectin
  • Common carp
  • blood biochemical

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مجله بهره برداری و پرورش آبزیان
دوره 13، شماره 3
مهر 1403
صفحه 129-140

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