بررسی میزان شیوع لیستریا مونوسایتوژنز و سالمونلا در ماهی، میگو و تخم‌مرغ ارائه شده در شهرستان شهرکرد، ایران

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 نویسنده مسئول، استاد گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران

2 دانشجوی دکتری تخصصی بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران.

3 دانشجوی دکتری تخصصی بهداشت مواد غذایی، گروه بهداشت مواد غذایی واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.

چکیده

امروزه کنترل میکروبیولوژیکی مواد غذایی به منظور جلوگیری از مسمومیت غذایی در انسان حائز اهمیت است. میکروارگانیسم‌های پاتوژن اعم از گرم مثبت و یا گرم منفی‌های بیماری‌زای موجود در موادغذایی است سبب گاستروآنتریت می‌شوند؛ لذا هدف از این مطالعه بررسی میزان آلودگی لیستریا مونوسایتوژنز و سالمونلا در ماهی، میگو و تخم‌مرغ‌های عرضه شده در شهرستان شهرکرد است. در این مطالعه تعداد 300 نمونه شامل100 نمونه ماهی، 100 نمونه میگو و 100 نمونه تخم‌مرغ به صورت تصادفی از خرده‌فروشی‌های شهرستان شهرکرد اخذ گردید و به آزمایشگاه انتقال داده شد. نمونه‌ها در محیط کشت غنی‌کننده لیستریا به مدت 48 ساعت غنی‌سازی شده و سپس بر روی محیط انتخابی کشت و پرگنه‌های مشکوک به لیستریا جداسازی از محیط کشت Palcam agar پس از تست-های تاییدی بیوشیمیایی شمارش شدند و برای تائید سالمونلا از محیط‌های کشت SS agar، BS agar و سپس TSIو LIA استفاده شد. نتایج به دست آمده نشان داد که از تعداد 100 نمونه ماهی 2 مورد (2 درصد)، از 100 نمونه میگو 4 مورد (4 درصد) و در 100 نمونه تخم‌مرغ تعداد 5 مورد (5 درصد) دارای آلودگی به لیستریا مونوسایتوژنز بود. میزان آلودگی به سالمونلا برای ماهی، میگو و تخم‌مرغ به ترتیب 9، 11 و 18 مورد آلودگی جداسازی شد. با توجه به شیوع لیستریا، پرهیز از مصرف غذاهای دریایی با پخت ناکافی توسط جمعیت‌های در معرض خطر توصیه می‌شود. اجرای دقیق شرایط بهداشتی سطوح در تماس با مواد غذایی و نواحی جابجایی، و شیوه‌های بهداشت فردی خوب باید آلودگی احتمالی محصولات شیلاتی توسط L. monocytogenes را کاهش داد.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation of the prevalence of Listeria monocytogenes and Salmonella in fish, shrimp and eggs presented in Shahrekord city, Iran

نویسندگان [English]

  • Ebrahim Rahimi 1
  • Mohammadamin Heidarzadi 2
  • Najmeh vahed dehkordi 3
1 Corresponding Author, Professor, Dept. of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
2 Ph.D. Student in Food Hygiene, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
3 Ph.D. Student in Food Hygiene, Dept. of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
چکیده [English]

Microbiological control of food is important to prevent food poisoning in humans. Pathogenic microorganisms are gram-positive or gram-negative pathogens found in food that cause gastroenteritis. The purpose of this study is to investigate the amount of Listeria monocytogenes and Salmonella in fish, shrimp and eggs presented in Shahrekord city.
Material and method: In this study, 300 samples, including 100 fish samples, 100 shrimp samples, and 100 egg samples, were randomly collected from retail stores in Shahrekord city and transferred to the specialized food hygiene laboratory of Shahrekord Islamic Azad University. The samples were enriched in Listeria enrichment culture medium for 48 hours and then on the selected culture medium and the suspected Listeria isolates from Palcam agar culture medium were counted after biochemical confirmation tests and for confirmation Salmonella was used from SS agar, BS agar and then TSI and LIA culture media. Research findings: The results showed that 2 out of 100 fish samples (2 percent), 4 out of 100 shrimp samples (4 percent), and 5 out of 100 egg samples (5 percent) were contaminated. It was Listeria monocytogenes. The amount of Salmonella contamination for fish, shrimp and eggs was 9, 11 and 18 respectively. Due to the prevalence of Listeria, it is recommended to avoid consumption of undercooked seafood by at-risk populations. Strict implementation of sanitary conditions of food contact surfaces and handling areas, and good personal hygiene practices should reduce possible contamination of seafood products by L. monocytogenes.

کلیدواژه‌ها [English]

  • Listeria monocytogenes
  • eggs
  • Shrimp
  • fish
  • Shahrekord

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مجله بهره برداری و پرورش آبزیان
دوره 12، شماره 2
تیر 1402
صفحه 145-156

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