ارزیابی ویژگی‌های بیوشیمیایی آنزیم‌ تریپسین تخلیص شده از روده ماهی کیلکای معمولی (Clupeonella cultriventris caspia)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 نویسنده مسئول، دانشیار گروه علوم و مهندسی شیلات، دانشکده منابع طبیعی و محیط زیست، دانشگاه ملایر، همدان، ایران

2 استاد گروه علوم و مهندسی شیلات، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، مازندران، ایران

3 استاد گروه بیوتکنولوژی، مؤسسه تحقیقات واکسن و سرم‌سازی رازی، کرج، البرز، ایران

چکیده

در این تحقیق، ویژگی‌های بیوشیمیایی آنزیم تریپسین تخلیص شده از روده ماهی کیلکای معمولی(Clupeonella cultriventris caspia) شامل دما و pH بهینه، دما و pH پایداری، اثر بازدارنده‌های آنزیمی، یون‌های فلزی، سورفاکتانت‌ها و عوامل اکسیدکننده مورد بررسی قرارگرفت. نتایج نشان داد دما و pH بهینه آنزیم تریپسین به ترتیب °C60 و 8 و پایداری دمایی و pH آن به ترتیب تا °C50 و 10-7 تعیین گردید. بازدارنده‌های SBTI و TLCK، به عنوان دو بازدارنده اختصاصی آنزیم تریپسین، اثر مهارکنندگی کاملی بر فعالیت آنزیم تریپسین داشتند (05/0>P). یون‌هایCa2+ وMg2+ سبب افزایش معنی‌دار و یون‌هایCu2+،Ba2+،Co2+،Zn2+،Fe2+ وAl3+ باعث کاهش معنی‌دار فعالیت آنزیم تریپسین گردیدند (05/0>P). یون‌هایK+ و Na+اثر معنی‌داری بر فعالیت آنزیم تریپسین نشان ندادند (05/0P>). فعالیت آنزیم تریپسین در حضور سورفاکتانت‌های ساپونین و سدیم کولات افزایش معنی‌دار (05/0>P) و در حضور سدیم دودسیل سولفات و عوامل اکسیدکننده (سدیم پربورات و پراکسید هیدروژن) کاهش معنی‌داری (05/0>P) را نشان داد. ویژگی‌های بیوشیمیایی آنزیم تریپسین نشان داد که این آنزیم می‌تواند در آینده برای استفاده در صنایع مخلتف مدنظر قرار گیرد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of biochemical properties of the purified trypsin from common kilka (Clupeonella cultriventris caspia) intestine

نویسندگان [English]

  • Abbas Zamani 1
  • Masoud Rezaei 2
  • Rasool Madani 3
1 Corresponding Author, Associate Prof., Dept. of Fisheries Sciences and Engineering, Faculty of Natural Resources and Environmental, Malayer University, Hamedan, Iran
2 Professor, Dept. of Fisheries Sciences and Engineering, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Mazandaran, Iran
3 Professor, Dept. of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Alborz, Iran.
چکیده [English]

In this work, the biochemical properties of the purified trypsin from common kilka (Clupeonella cultriventris caspia) intestine including optimum temperature and pH, thermal and pH stability, effect of inhibitors, metal ions, oxidizing agents and surfactants were assayed. According to the obtained results, optimum temperature and pH of the trypsin were recorded at 60°C and 8 respectively. The stability of the trypsin was well preserved at temperatures of up to 50°C and pH from 7.0 to10.0. SBTI and TLCK, two specific trypsin inhibitors, had a completely inhibition effect on the enzymatic activity (P<0.05). The enzyme activity was significantly increased in the presence of Ca+2 and Mg+2 and decreased by Cu+2, Ba+2, Co+2 , Zn+2 , Fe+2 and Al+3 (P<0.05). Na+and K+ did not show any significant effect on the activity of trypsin (P>0.05). The enzymatic activity was significantly increased in presence of surfactants including saponin and sodium cholate and showed a significant decrease in presence of SDS and oxidizing agents like sodium perborate and hydrogen peroxide (P<0.05). Therefore, the results of our study can contribute to the clear understanding of the purified trypsin from common kilka intestine for application in different industries in future.

کلیدواژه‌ها [English]

  • Trypsin
  • intestine
  • common kilka
  • biochemical properties

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مجله بهره برداری و پرورش آبزیان
دوره 11، شماره 3
مهر 1401
صفحه 41-56

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