بررسی اثر خوراکی جلبک گراسیلاریا کورتیکاتا بر سیستم ایمنی میگوی سفید غربی درمواجهه با ویروس لکه سفید

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 پژوهشکده آبزی‌پروری جنوب کشور، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران.

2 نویسنده مسئول، پژوهشکده آبزی‌پروری جنوب کشور، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران.

3 مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

4 پژوهشکده آبزی‌پروری جنوب کشور، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران

چکیده

با هدف پیشگیری از بیماری لکه‌سفید میگو با استفاده از جلبک گراسیلاریا کورتیکاتا (Gracilaria corticata)، تعداد 300 عدد میگوی پیش مولد وانامی در دو گروه با عصاره جلبک (A) و دو گروه با غذای تجاری بدون عصاره (کنترل) به مدت 14 روز تغذیه شدند. درپایان روز چهاردهم یک گروه از میگوهایی که با عصاره تغذیه شده بودند (AV) و یک گروه از میگوهای کنترل ( کنترل مثبت) با ویروس لکه سفید مواجه شدند. پس از روز چهاردهم طی روزهای 0، 3، 9، 18 و 25 میزان بازماندگی و فاکتورهای ایمنی میگوها بررسی گردید. بر اساس نتایج میگوهای تغذیه‌شده با عصاره جلبک در مواجهه با ویروس لکه سفید (AV) در مقایسه با میگوهای تغذیه‌شده با غذای تجاری بدون عصاره در مواجهه با ویروس (کنترل مثبت) به‌طور معنی‌داری (05/0P<) از بقا بیشتری برخوردار بودند (b 5/0 ± 5/22). افزایش فاکتورهای ایمنی از روز اول تا روز 25 مشاهده و بیشترین میزان فاکتورهای ایمنی THC، TPP، SOD، POD و PO در گروه A در روز 25 آزمایش مشاهده شد. این وضعیت برای گروه AV نیز صادق بوده ولی میزان آن به نسبت گروه A کمتر و با هم اختلاف معنی‌دار داشتند (05/0P<). با توجه به عملکرد جلبک در تحریک سیستم ایمنی، به نظر می‌رسد می‌توان در طول دوره پرورش از مکمل جلبک در جیره غذایی به عنوان راهکاری پیشنهادی در کنترل بیماری لکه سفید استفاده نمود.

کلیدواژه‌ها

عنوان مقاله [English]

Dietary supplementation of Gracilaria corticata extracts improved immunity of white leg shrimp exposed to white spot virus

نویسندگان [English]

  • Hossein Houshmand 1
  • Mina Ahangarzadeh 2
  • Seyed Reza Seyedmortezaei 3
  • Mehrdad Mohammadidust 4
  • Shapoor Kakoolaki 3
  • Lefteh Mohseninejad 1
1 South Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.
2 Corresponding Author, South Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran
3 Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
4 South Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran
چکیده [English]

In order to investigate the resistance of shrimp against white spot disease by Gracilaria corticata algae, 300 Vannamei shrimp in two groups were fed with and without algae extract for 14 days. At the end of the experiment, half of the shrimp of each group were exposed to the white spot virus. After the challenge, during days 0, 3, 9, 18 and 25, the survival rate and immune factors of shrimp were examined. According to the results, survival rate of shrimp fed with algae extract was higher than the shrimp were fed by commercial pellet without the algae extract (positive control) significantly (P < 0.05). Immune system enhancement from the first to twenty fifth day and the highest levels of THC, TPP, SOD, POD and PO were observed in group Algae on twenty fifth day of the experiment. This condition was also observed in group AV, but less than group A with a significant difference (P <0.05). Considering the function of algae in stimulating the immune system, it seems that during the culture period, algae supplementation in the diet can be used as a proposed strategy to control white spot disease.

کلیدواژه‌ها [English]

  • White spot disease
  • Gracilaria corticata
  • algae
  • Immunity
  • vannamei

مراجع

1.Afsharnasab, M., Mortezaei. S.R., Ahangarzadeh, M., Houshmand, H., Kor, N., Jorfi, E., Sabzalizadeh, S., and Mohseni, L. 2009. Assessing the health and diseases status of shrimp breeding centers in Iran, Iranian Fisheries Science Research Institute (In Persian)
2.Afsharnasab, M., Mortezaei, S.R., Eskandari, Gh., Kor, N., Jorfi, E., and Lalouei, F. 2006. Investigation and determination of white spot disease Source in farmed shrimps in Abadan. Iranian Fisheries Science Research Institute, 75p. (In Persian)
3.Statistical Yearbook, 2019. The amount of farmed shrimp. Iran Fisheries Organization. 64p. (In Persian)
4.Chang, P.S., Chen, H.C., and Wang, Y.C. 1998. Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crabs and lobsters by in situ hybridization. Aquaculture. 164: 233-242.
5.Di Leonardo, V.A., Bonnichon, V., Roch, P., Parrinello, N., and Bonami, J.R. 2005. Comparative WSSV infection routes in the shrimp genera Marsupenaeus and Palaemon. Journal of Fish Diseases.28: 565-569.
6.Escobedo-Bonilla, C.M., Wille, M., Alday-Sanz, V., Sorgeloos, P., Pentair, M.B., and Nauwynck, H.J. 2007. Pathogenesis of a Thai strain of white spot syndrome virus (WSSV) in SPF Litopenaeus vannamei. Diseases of Aquatic Organisms. 74: 85-94.
7.Witvrouw, M., and De Clercq, E. 1997. Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. General Pharmacology. 29: 497-511.
8.Eluvakkal, T., Sivakumar, S.R., and Arunkumar, K. 2010. Fucoidan in some Indian brown seaweeds found along the Coast Gulf of Mannar. International Journal of Botany. 6: 2. 176-81.
9.Patankar, M.S., Oehninger, S., Barnett, T., Williams, R.L., and Clark, G.F.1993. A revised structure for fucoidan may explain some of its biological activities. Journal of Biological Chemistry, 268: 29. 21770-6.
10.Berteau, O., and Mulloy, B. 2003. Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide. Glycobiology,13: 29-40.
11.Chotigeat, W., Tongsupa, S., Supamataya, K., and Phongdara, A. 2004. Effect of fucoidan on disease resistance of black tiger shrimp. Aquaculture. 233: 23-30.
12.Rameshthangam, P., and Ramasamy, P. 2007. Antiviral activity of bis(2-methylheptyl) phthalate isolated from Pongamia pinnata leaves against White spot syndrome virus of Penaeus monodon Fabricus. Virus Research,126: 38-44.
13.Yi, G., Qian, J., Wang, Z., and Qi, Y. 2003. A phage-displayed peptide can inhibit infection by white spot syndrome virus of shrimp. Journal of General Virology, 84: 2545-2553.
14.Jaime Ceballos, B., Villareal, H., Garcia, T., Pérez Jar, L., and Alfonso, E. 2005. Effect of Spirulina platensis meal as feed additive on growth, survival and development in Litopenaeus schmitti shimp larvae. Rev. Invest. Mar.26: 3. 235-241.
15.Rajasekar, T., Deivasigamani, B., Kumaran, S., Sakthivel, M., Balamurugan, S., Jothi, E.G., and Priyadharshini, P. 2012. Antibacterial Activity of Cultivated Marine Seaweeds against Fish pathogenic bacteria Vibrio harvey. Asian Pac. J. Trop. Biomed.pp. 1-5.
16.Rodriguez-Bernaldo De Quiros, A., Lage-Yusty, M., and Lopez-Hernandez, J. 2010. Determination of phenolic compounds in macroalgae for human consumption. Food Chem. 121: 634-638.
17.Lin, Y.C., Yeh, S.T., Li, C.C.,Chen, L.L., Cheng, A.C., and Chen,J.C. 2011. An immersion of Gracilaria tenuistipitata extract improves the immunity and survival of white shrimp Litopenaeus vannamei challenged with white spot syndrome virus. Fish & Shellfish Immunology, 31: 1239-1246.
18.Balasubramanian, G., Sarathi, M., Rajesh Kumar, S., and Sahul Hameed, A.S. 2007. Screening the antiviral activity of Indian medicinal plants against white spot syndrome virus in shrimp. Aquaculture. 263: 15-19.
19.Shiroma, R., KoniShi, T., Uechi, S., and TaKo, M. 2008. Structural study of fucoidan from the brown seaweed Hizikia fusiformis. Food science and technology research. 14: 176-182.
20.Afsharnasab, M., Mortezaei, R., Yegane, V., and Kazemi, B. 2009. Gross sign, histopathology and polymerase chain reaction observations of white spot syndrome virus in shrimp specific pathogen free Litopeneaus vannamei in Iran. Asian Journal of Animal and Veterinary Advances. 4: 297-305.
21.Van Hulten, MC.W., Tsai, M.F., Schipper, C.A., Lo, C.F., Kou, G.H., and Valk, J.M. 2000. Analysis of a genomic segment of white spot syndrome virus of shrimp containing ribonucleotide reductase genes and repeat regions, J. Gen. Virol. 81: 307-316.
22.Yoganandhan, K.S.S., Murugan, V., Narayanan, R.B., and Sahul Hameed, A.S. 2003. Screening the organs for early detection of white spot syndrome virus in Penaeus indicus by histopathology and PCR techniques. Aquaculture, 215: 21-29.
23.Kakoolaki, S., Sharifpour, I., Soltani, M., Ebrahimzadeh Mousavi, H., Mirzargar, S., and Rostami, M. 2010. Selected morpho-chemical features of hemocytes in farmed shrimp, Fenneropenaeus indicus in Iran. Iranian Journal of Fisheries Sciences. 9: 219-232.
24.Huang, J., Yang, Y., and Wang, A.2010. Reconsideration of phenoloxidase activity determination in white shrimp Litopenaeus vannamei. Fish & Shellfish Immunology, 28: 240-244.
25.Bradford, M.M. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-Dye Binding. Anal. Biochem. 72: 248-254.
26.Valderrama, D., and Anderson, J.L. 2011. Shrimp production review. Food and Resource Economics Department, University of Florida, USA. Santiago, Chile, November 6-9.
27.Afsharnasab, M., Houshmand, H., Ahangarzadeh, M., and Mortezaei, S.R., 2016. Assessing the sustainability of western white leg shrimp white spot disease vaccinated and fed with yeast and algae Sacchromayces cerevisiae Gracilaria corticata in the face ofwhite spot disease virus, Iranian Fisheries Science Research Institute, 110p. (In Persian)
28.Verbruggen, B., Bickley, L.K., Van Aerle, R., Bateman, K.S., Stentiford, G.D., Santos, E.M., and Tyler, C.R. 2016. Molecular Mechanisms of White Spot Syndrome Virus Infection and Perspectives on Treatments. Viruses 8, 23: 1-29.
29.Wang, X.W., and Wang, J.X. 2013. Pattern recognition receptors acting in innate immune system of shrimp against pathogen infections. Fish & Shellfish Immunology, 34: 981-989.
30.Immanuel, G., Sivagnanavelmurugan, M., Marudhupandi, T., Radhakrishnan, S., and Palavesam, A. 2012. The effect of fucoidan from brown seaweed Sargassum wightii on WSSV resistance and immune activity in shrimp Penaeus monodon (Fab). Fish and Shellfish Immunology. 32: 551-564.
31.Sirirustananun, N., Chen, J.C., Lin, Y.C., Yeh, S.T., Liou, C.H., Chen, L.L., Sim, S.S., and Chiew, S. 2011.Dietary administration of a Gracilaria tenuistipitata extract enhances the immune response and resistance against Vibrio alginolyticus and white spot syndrome virus in the white shrimp Litopenaeus vannamei. Fish and Shellfish Immunology. 31: 848-855.
32.Wongprasert, K., Rudtanatip, T., and Praiboon, J. 2014. Immunostimulatory activity of sulfated galactans isolated from the red seaweed Gracilaria fisheri and development of resistance against white spot syndrome virus (WSSV) in shrimp. Fish & Shellfish Immunology, 36: 52-60.
33.Wang, F.I., and Chen, J.C. 2006. Effect of salinity on the immune response of tiger shrimp Penaeus monodon and
its susceptibility to Photobacterium damselae subsp. damselae. Fish Shellfish Immunol. 20: 671-81.
34.Kumar, S., Ahamed, V., Sarathi, M.,Basha, A., and Hameed, A. 2008. Immunological responses of Penaeus monodon to DNA vaccine and its efficacy to protect shrimp against white spot syndrome virus (WSSV). Fish and Shellfish Immunology, 24: 4. 467-478.
35.Balasubramanian, G., Sarathi, M., Venkatesan, C., Thomas, J., and Sahul Hameed, A.S. 2008. Studies on the immune modulatory effect of extract of Cyanodon dactylon in shrimp, Penaeus monodon, and its efficacy to protect the shrimp from white spot syndrome virus (WSSV). Fish & Shellfish Immunology, 25: 820-828.
36.Chen, J.C., and Cheng, S.Y. 1993. Studies on hemocyanin and haemolymph protein levels of Penaeus japonieus based on sex, size and moulting cycle. Comp Biochem Physiol. 106B: 293-296.
37.Le Moullac, G., and Haffner, P. 2000. Environmental factors affecting immune responses in Crustacea. Aquaculture, 191: 121-131.
38.Wang, D.L., Zuo, D., Wang, L.M., Sun, T., Wang, Q., and Zhao, Y.L. 2012. Effects of white spot syndrome virus infection on immuno-enzyme activities and ultrastructure in gills of Cherax quadricarinatus. Fish and Shellfish Immunology. 32: 645-650.
39.Yan, X., Jie, A., Jing-Qiu, S.,Ya-Dong, Y. and Jun-Qiang, Y. 2010. Cytochemical location of superoxide dismutase and peroxidase in different tissues of Litopenaeus vannamei. Acta Hydrobiologica Sinica, 34: 2. 402-409.
40.Pacheco, R., Ascencio, F., Zarain, M., Gómez, G., and Campa, A. 2011. Enhancement of superoxide dismutase and catalase activity in juvenile brown shrimp, Farfantepenaeus californiensis (Holmes, 1900), fed β-1.3 glucan vitamin E, and β-carotene and infected with white spot syndrome virus. Lat. Am. J. Aquat. Res. 39: 3. 534-543.
41.Van De Braak, C.B.T. 2002. Haemocytic defence in black tiger shrimp (Penaeus monodon). PhD thesis, Wageningen University, Wageningen Institute of Animal Sciences. PO Box 338, 6700 AH Wageningen, The Netherlands., 168p.
42.Citarasu, T., Sivaram, V., Immanuel, G., Rout, N., and Murugan, V. 2006. Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes. Fish and Shellfish Immunology, 21: 372-384.
43.Chen, Y.Y., Chen, J.C., Lin, Y.C., Yeh, S.T., and Huang, C.L. 2015. White Shrimp Litopenaeus vannamei That Have Received Gracilaria tenuistipitata Extract Show Early Recovery of Immune Parameters after Ammonia Stressing. Mar. Drugs. 13: 6. 3606-3624.
44.Sun, C.B., Wang, G., and Chan, S.F. 2015. Effects of artificial infection of Litopenaeus vannamei by Micrococcus lysodeikticus and WSSV on the activity of immunity related enzymes. Fish and Shellfish Immunology, 46: 778-786.
45.Wen-Ying, S., Hui-Jun, Y., Hui-Feng, K.E., and Lan, Q.I. 2007. Effect ofβ-glucan on Enzyme Activity of Immunity in Pacific White Leg Shrimp Litopenaeus vannmei. Fisheries Science, 2007-07.
مجله بهره برداری و پرورش آبزیان
دوره 11، شماره 2
تیر 1401
صفحه 101-117

فایل ها

هم رسانی

ارجاع به این مقاله

آمار