اثر میکروبیوم حاوی باکتری های Nitrosomonas oligotropha و Nitrobacter winogradskyi بر پرورش ماهی قزل‌لای رنگین‌کمان (Onhorhynchus mykiss)

نوع مقاله : مقاله کامل علمی - پژوهشی


1 نویسنده مسئول، استادیار گروه دامپزشکی علوم دامی، پژوهشکده کشاورزی هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی، کرج- ایران.

2 استاد گروه شیلات، دانشکده منابع طبیعی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 دانشیار گروه دامپزشکی علوم دامی، پژوهشکده کشاورزی هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی، کرج- ایران.


هدف از انجام این پژوهش، بکارگیری میکروبیوم غنی سازی شده با باکتریهای تجزیه کننده آمونیاک و نیتریت در سیستم مداربسته قزل‌آلای رنگین کمان بود. مجموعه باکتریایی (PAN)حاوی Nitrosomonas oligotropha و Nitrobacter winogradskyi در یک سیستم مداربسته پرورش ماهی قزل آلا (50 گرم) در آزمایشگاه آبزیان گروه شیلات دانشگاه تهران اضافه شد و اثر آن روی شاخصهای خون‌شناسی (هموگلوبولین، هماتوکریت، گلبول قرمز، گلبول سفید، MCV، MCHC، لنفوسیت، مونوسیت و نوتروفیل)، ایمنی شناسی (لیزوزیم، ایمنوگلوبولین، کمپلمان و انفجار تنفسی) و شاخصهای سنجش استرس (هورمون کورتیزول و گلوکز) و کیفیت آب پرورشی بچه ماهی قزل آلای رنگین کمان (Onhorhynchus mykiss) در قالب یک طرح کاملاً تصادفی مورد بررسی قرار گرفت. در این آزمایش یک گروه کنترل منفی (NC) نیز برای مقایسه با گروه آزمایشی در نظر گرفته شد. نتایج نشان داد که با به کار گیری میکروبیوم PAN غلظت آمونیاک و نیتریت تولید شده به طور معنی داری کاهش و در نتیجه این منجر به کاهش استرس و کاهش سرکوب سیستم ایمنی و بهبود رشد شده است. بنابراین استفاده از این گروه باکتریایی در بهبود سیستم پرورشی ماهی قزل آلای رنگین کمان رای سیستم پرورش قزل آلای رنگین کمان پیشنهاد می شود.


عنوان مقاله [English]

The effect of microbiome containing Nitrosomonas oligotropha and Nitrobacter winogradskyi bacteria on the breeding of rainbow trout (Onhorhynchus mykiss)

نویسندگان [English]

  • Alireza Neissi 1
  • Gholamreza Rafiee 2
  • Gholamreza Shah Hoseini 3
1 Corresponding Author, Assistant Prof., Dept. of Veterinary Animal Sciences, Nuclear Agricultural School, Nuclear Science and Technology Research Institute, Atomic Energy Organization, Karaj, Iran.
2 Professor, Dept. of Fisheries Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
3 Associate Prof., Dept. of Veterinary Animal Sciences, Nuclear Agricultural School, Nuclear Science and Technology Research Institute, Atomic Energy Organization, Karaj, Iran.
چکیده [English]

he aim of this study was to use a microbiome enriched with ammonia and nitrite oxidizing bacteria in the rainbow trout RAS system. A bacterial microbiome containing Nitrosomonas oligotropha and Nitrobacter winogradskyi (PAN) was added to a rainbow trout culture system (50 g) in the Aquatic Laboratory of the Fisheries Department of the University of Tehran and its effect on hematological parameters (hemoglobulin, hematocrit, erythrocytes, white blood cells, MCV, MCHC, lymphocytes, monocytes, and neutrophils) and stress (cortisol and glucose) as well as water quality of rainbow trout (Oncorhynchus mykiss) in a completely randomized design. In this experiment, a negative control (NC) group was also included for comparison with the experimental group. The results showed that by using PAN microbiome, the concentration of ammonia and nitrite produced was significantly reduced and as a result, this led to a reduction in stress and a reduction in immune suppression and improved growth. Therefore, the use of this bacterial group in improving the breeding system of rainbow trout is suggested by the rainbow trout breeding system.

کلیدواژه‌ها [English]

  • rainbow trout
  • microbiome
  • ammonium and nitrite oxidizing bacteria
  • hematology and immunology indices
  • Stress indices
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