بررسی تاثیر محیط کشت اختصاصی حاوی عصاره سیب زمینی و موز به عنوان پری بیوتیک روی رشد مخمر ساکارومایسیس سرویسیه (Saccharomyces cerevisiae) در شرایط آزمایشگاهی

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان. ایران

2 گروه تکثیر و پرورش آبزیان- دانشکده شیلات و محیط زیست- دانشگاه علوم کشاورزی و منابع طبیعی گرگان

3 دانشگاه علوم کشاورزی و منابغ طبیغی گرگان

4 گروه علوم آزمایشگاهی، دانشکده پیراپزشکی – دانشگاه علوم پزشکی گلستان

چکیده

هدف از تحقیق حاضر تأثیر خاصیت پری بیوتیکی سیب زمینی و موز روی رشد مخمر ساکرومایسس سروسیه (Saccharomyces cerevisia) بود. برای انجام این آزمایش، مخمر به ‌صورت لیوفیلیزه تهیه و پس از کشت در محیط کشت مایع سابرو دکستروز، روی محیط کشت جامد کشت داده شد؛ سپس کلنی‌های به‌دست آمده به محیط کشت اختصاصی فاقد گلوکوز و حاوی غلظت‌های 2، 25/6، 5/12، 25، 50، 75 و 100 درصد از عصاره سیب زمینی و موز به عنوان پری بیوتیک، تلقیح شدند و میزان رشد آن مورد سنجش قرار گرفت. میزان رشد میکروارگانیسم با توجه به منحنی رشد بررسی و جذب نوری به ‌وسیله دستگاه اسپکتروفتومتر در طول موج600 نانومتر قرائت گردید. نتایج به دست آمده نشان داد که رشد مخمر در هر دو محیط حاوی عصاره‌های موز و سیب زمینی با غلظت 100 درصد افزایش معناداری در مقایسه با سایر تیمارها داشت؛ همچنین بیشترین میزان رشد تیمارهای حاوی عصاره موز و سیب زمینی با غلظت 100 درصد با یکدیگر اختلاف معنادار داشتند. با توجه به نتایج حاصل از این تحقیق، می‌توان از عصاره موز و سیب زمینی به عنوان ماده‌ای با خاصیت پری بیوتیکی و ارزان برای رشد مخمر ساکرومایسس سرویسیه استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

In vitro effect of specific culture medium containing potato and banana extracts as prebiotics on the growth of yeast (Saccharomyces cerevisiae)

نویسندگان [English]

  • Mohammad Sudagar 1
  • Bita Sadat Seyedalangi 2
  • Mohammad Mazandarani 1
  • Hamideh Zakariaee 3
  • seyedeh Sedigheh hoseini 4
1 Department of Aquaculture, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources. Iran
2 Department of Aquaculture, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources. Iran.
3 gorgan university
4 Golestan
چکیده [English]

The aim of this study was to determine the effect of prebiotic properties of potato and banana on the growth of Saccharomyces cerevisia yeast. To perform this experiment, S. cerevisia was prepared as lyophilized and after culturing in Sabouraud dextrose broth medium, it was cultured on agar culture medium; the obtained colonies inoculated to the free-glucose media containing concentrations of 2, 6.25, 12.5, 25, 50, 75 and 100 percent of banana and potato extractions as prebiotics; then, the growth rate of S. cerevisia was measured according to the growth curve and optical density was read by spectrophotometer at a wavelength of 600 nm. The results showed that the growth of S. cerevisia in both media containing banana and potato extracts at a concentration of 100% had a significant increase compared to other treatments; Also, the highest growth rate of treatments containing banana and potato extract with 100% concentration were significantly different from each other. According to the results of this study, banana and potato extract can be used as a prebiotic substance for the growth of Saccharomyces cerevisiae.

کلیدواژه‌ها [English]

  • Yeast Saccharomyces cerevisiae
  • potatoes
  • bananas
  • Prebiotic
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