اثرات سطوح مختلف پکتین بعنوان پربیوتیک طبیعی در جیره غذایی بر ایمنی، سیستم دفاع آنتی‌اکسیدانی میگوی پا سفید غربی (Litopenaeus vannamei )

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 عضو هیات علمی گروه تکثیر و پرورش آبزین، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، استان گلستان، ایران

2 رئیس اداره توسعه میگو

3 استادیار دانشگاه علوم کشاورزی و منابع طبیعی گرگان

4 عضو هیئت علمی

5 عضو هیات علمی

چکیده

هدف از مطالعه حاضر بررسی اثرات پکتین استخراج شده از پوست پرتقال به عنوان یک پربیوتیک طبیعی بر شاخص‌های همولنف، شاخص‌های ایمنی غیر اختصاصی، سیستم دفاع آنتی‌اکسیدانی، گوارشی میگوی پا سفید غربیLlitopenaeus vannamei بود. بدین منظور میگوی پا سفید غربی با میانگین وزنی 3 گرم و در چهار تیمار؛ شاهد(بدون پکتین)، و وسه تیمار 5/0، 1 و 5/1 درصد پکتین بودند. میگو‌ها به مدت 2 ماه با جیره‌های آزمایشی تغذیه شدند. مصرف پکتین سبب بهبود تعداد هموسیت کل در همه گروه‌های تغذیه شده با پکتین، هموسیت هیالینه در گروه تغذیه شده با پکتین 1 درصد و هموسیت گرانولار بزرگ در گروه تغذیه شده با سطح 5/1 درصد پکتین نیز گردید (05/0 > P). ولی بر بازماندگی میگو‌ها و میزان لیزوزیم، پروتئین کل و گلوکز اثر معنی‌داری نداشت (05/0 > P). آنزیم‌های آنتی‌اکسیدانی در پژوهش حاضر نشان دهنده افزایش فعالیت کاتالاز و سوپراکسید دیسموتاز در تیمارهای 5/0 و 5/1 درصد پکتین بود. از نظر آماری، میزان فعالیت آنزیم مذکور در تیمار با پکتین 1 درصد و تیمار شاهد تفاوت معنی‌داری نداشت(05/0 > P). بیشترین میزان فعالیت فنول ‌اکسیداز، مربوط به گروه تغذیه شده با پکتین 1 درصد بود. اما میزان فعالیت این آنزیم در دو تیمار 5/0 و 5/1 درصد تفاوت معنی‌داری با گروه شاهد نداشتند(05/0 > P). نتایج نشان داد، پکتین به عنوان یک پر‌بیوتیک طبیعی در تحریک دفاع آنتی-اکسیدانی، و بهبود سیستم ایمنی موثر هستند.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of different levels of pectin as a natural prebiotic in the diet on the immune system, antioxidant defense system of leg white shrimp (Liptopenaeus vannamei).

نویسندگان [English]

  • Valiollah Jafari 1
  • hamid reza nasiri 2
  • Seyed Hossein Hoseinifar 3
  • mahammd mazandarani 4
  • Hien van Doan 5
1 Faculty member of Aquatic Breeding Department, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Golestan Province, Iran
2 Head of Shrimp Development Department
3 Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of 6 7 Agricultural Sciences and Natural Resources, Gorgan, Iran.
4 Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of 6 7 Agricultural Sciences and Natural Resources, Gorgan, Iran.
5 Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, 8 9 Chiang Mai 50200, Thailand
چکیده [English]

The aim of this study was to investigate the effects of pectin extracted from orange peel as a natural prebiotic on hemolymph markers, nonspecific immune markers, antioxidant defense system, and digestive function of Llitopenaeus vannamei. the group fed with 1.5% pectin level (P <0.05). However, it had no significant effect on shrimp survival and lysozyme content, total protein and glucose (P >0.05). Antioxidant enzymes in the present study showed an increase in catalase and superoxide dismutase activity in 0.5 and 1.5% pectin treatments. Statistically, the activity of this enzyme in treatment with 1% pectin and control treatment was not significantly different (P >0.05). The highest phenol oxidase activity was in the group fed with 1% pectin. However, the activity of this enzyme in the two treatments of 0.5 and 1.5% were not significantly different from the control group (P >0.05). The results showed that pectin as a natural probiotic is effective in stimulating antioxidant defense, and improving the immune system.

کلیدواژه‌ها [English]

  • Leg white shrimp
  • nonspecific immune system
  • Antioxidant system
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