تاثیر سطوح مختلف پکتین حاصل از تفاله سیب درختی (Malus pumila) بر شاخص‌های خون‌شناسی و بیوشیمیایی ماهی قزل آلای رنگین کمان (Oncorhynchus mykiss)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 گروه بهداشت و بیماری‌های آبزیان، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

3 گروه شیلات، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران

4 گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

5 گروه شیلات و علوم دامی، دانشکده کشاورزی، دانشگاه چیانگ مای، تایلند

6 گروه تولیدات دامی، دانشکده کشاورزی، دانشگاه قاهره، مصر

7 دانشکده علوم و تکنولوژی، دانشگاه سانیو، ایتالیا

چکیده

در این تحقیق تاثیر پکتین حاصل از تفاله سیب درختی (Malus pumila) روی شاخص‌های خون‌شناسی و بیوشیمیایی ماهی قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss) مورد بررسی قرار گرفت. در این بررسی 300 قطعه ماهی قزل‌آلا با میانگین وزنی 007/0±56/3 گرم با جیره‌های غذایی به ترتیب حاوی 5/0، 1 و 2 درصد پکتین در کیلوگرم جیره و گروه شاهد با جیره فاقد پکتین به مدت 6 هفته تغذیه شدند. در پایان دوره، شاخص‌های خون‌شناسی و بیوشیمیایی بر اساس فرمول‌های استاندارد محاسبه و ارزیابی انجام گرفت. بر اساس نتایج بدست آمده از شاخص‌های خون‌شناسی‌، تیمار تغذیه شده با 5/0 و 1 درصد پکتین دارای بیشترین میزان هماتوکریت، هموگلوبین و گلبول سفید بوده و اختلاف معنی‌داری با تیمار شاهد نشان دادند (05/0p <). در سایر شاخص‌های خونی (گلبول‌های قرمز، MCV و MCH) اختلاف معنی‌داری بین تیمارهای آزمایشی و گروه شاهد مشاهده نشد (05/0< p). تغذیه ماهیان با جیره حاوی پکتین بطور معنی‌داری سبب افزایش شاخص آلبومین و کاهش شاخص‌های کلسترول، گلوکز و کورتیزول شد (05/0p <). در سایر شاخص‌های بیوشیمیایی خون (پروتئین کل، گلوبولین و تری‌گلیسیرید) اختلاف معنی‌داری بین سطوح مختلف پکتین و گروه کنترل مشاهده نشد (05/0< p). بنابراین می‌توان اینگونه نتیجه گرفت که استفاده از پکتین در جیره غذایی ماهی قزل‌آلای رنگین کمان منجر به بهبود برخی از فاکتورهای خون‌شناسی و بیوشیمیایی می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of different levels of pectin from Malus Pumila pomace on hematological and biochemical parameters of rainbow trout (Oncorhynchus mykiss)

نویسندگان [English]

  • Seyed Hossein Hoseinifar 1
  • Mahdi Soltani 2
  • Ghassem Rashidian 3
  • Hamed Ghafarifarsani 4
  • Mohammad Amin Jahazi 1
  • Hien Van Doan 5
  • Ehab El-Haroun 6
  • Marina Paolucci 7
1 Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Dept. of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Dept. of Fisheries, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
4 Dept. of Fisheries, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Iran
5 Department of Fisheries and Animal Sciences, Faculty of Agriculture, Chiang Mai University, Thailand
6 Department of Animal Production, Faculty of Agriculture, Cairo University, Egypt
7 Faculty of Science and Technology, University of Sanio, Italy
چکیده [English]

The aim of this study was to investigate the effects of apple pomace (Malus pumila) derived pectin (APDP) on rainbow trout (Oncorhynchus mykiss). Therefore, 300 rainbow trout with an average weight of 3.56 ± 0.007 were fed diets containing 0.5, 1 and 2% pectin per kg of diet and the control group was fed a diet without pectin for 6 weeks. At the end of the experiment, hematological and biochemical indices were evaluated based on standard formulas and means were analyzed using Tukey test. Based on the results obtained from hematological indices, the treatment fed with 0.5 and 1% of pectin showed the highest levels of hematocrit, hemoglobin and numbers of white blood cells significantly different from those of control treatment (p < 0.05). In terms of other blood indices (erythrocytes, MCV and MCH), no significant difference was found between the experimental treatments and control group (P>0.05). Feeding fish with pectin-containing diets significantly increased albumin index and decreased cholesterol, glucose and cortisol indices (p < 0.05). In case of total protein, globulin and triglyceride, no significant difference was observed between different levels of pectin and control group (P>0.05). Therefore, it can be concluded that the use of pectin in the diet of rainbow trout leads to the improvement of some hematological and biochemical indices

کلیدواژه‌ها [English]

  • Prebiotics
  • Agricultural wastes
  • Stress indices
  • Rainbow trout

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مجله بهره برداری و پرورش آبزیان
دوره 10، شماره 1
فروردین 1400
صفحه 59-70

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