اثرات تغذیه‌ای عصاره‌ی هیدروالکلی آویشن (Thymus vulgaris) بر مقاومت و برخی فراسنجه های بیوشیمیایی خون در بچه ماهیان کپور معمولی (Cyprinus carpio Linnaeus, 1758) در مواجهه با تنش شوری

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد گروه تکثیر و پرورش شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی، گرگان، ایران

2 دانشیار گروه تکثیر و پرورش شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی، گرگان، ایران

چکیده

به‌منظور افزایش مقاومت ماهیان در برابر استرس‌های محیطی؛ می‌توان از افزودنی‌های تغذیه ای در جیره‌ی غذایی ماهیان استفاده کرد. در این خصوص، تعداد 180ماهی با میانگین وزنی (063/0±84/10) گرم در چهار تیمار و با 3 تکرار و 15 قطعه ماهی در هر تکرار و با جیره‌های حاوی صفر، 5/0، 1، و 2 درصد عصاره گیاه -آویشن در مدت 70 روز تغذیه، بررسی شدند. در پایان دوره 30 قطعه ماهی از هر تیمار به مدت 12 ساعت تحت تنش شوریppt 13 (برای بررسی پارامترهای بیوشیمیایی) و 14 (برای سنجش بقا و میزان مقاومت) قرار گرفتند. به‌این منظور خون‌گیری در زمان‌های قبل از تنش، 6، 24 ،72 و 168ساعت پس از تنش انجام شد. در این بررسی افزودن عصاره گیاه آویشن در مدت پرورش، اثر معنی‌داری بر مقاومت ماهی در مواجهه با تنش شوری نداشت (05/0P>). مقادیر کورتیزول، گلوکز، پروتئین کل، آلبومین قبل از تنش شوری اختلاف معنی‌دار بین تیمارها نشان ‌نداد (05/0P>). با افزایش شوری کورتیزول و گلوکز نیز افزایش یافتند که همه‌ی تیمارها تغییر معنی-داری نسبت به قبل ازتنش شوری نشان دادند(05/0p <).در این بررسی آلبومین و پروتئین کل اختلاف معنی‌داری در زمان‌های مختف خون‌گیری نداشتند (05/0< p). بر اساس نتایج بررسی حاضر افزودن عصاره آویشن در سطوح 5/0، 1 و 2 درصد جیره تاثیری مقاومت ماهی و نیز فراسنجه‌های بیوشیمیایی خونی ماهی کپورمعمولی در مواجهه با استرس شوری نداشت.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of dietary Thyme (Thymus vulgaris) extract on the Resistance and some biochemical parameters in Common carp (Cyprinus carpio) to salinity stress.

نویسندگان [English]

  • Zeinab Reza gholitabar 1
  • Valiallah Jafari 2
  • Mohammad Mazandarani 2
1 1M.Sc. Student, Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran,
2 Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

In order to survey the effect of Thyme (Thymus vulgaris) extract on the common carp , 180 fish with the average weight of 10.84±0.063 were randomly divided into four groups (with Thyme extract added in 0, 0.5, 1 and 2 % to the fish diet) in three replicates (each with 15 fish) for 70 days. At the end of this period, 30 fish from each group were exposed to the seawater with 13 and 14 ppt for 12 hours to survey biochemical parameters and survival rate and resistance, respectively. In this regard, blood samples were collected before salinity stress and 6, 24, 72 and 168 hours after salinity stress. Our results showed that added Thyme supplement into the food has no effect on fish resistance during the experimental period (P>0.05). Cortisol, glucose, total protein, albumin showed no significant differences between treatments before salinity stress (P> 0.05). However, increasing salinity increased cortisol and glucose significantly in all treatments compared to pre-salinity stress (P <0.05). Albumin and total protein did not show any significant difference in any treatments (P >0.05). Based on the results of the present study, adding thyme extract at 0.5, 1 and 2% into the diet did not have any effect on fish resistance and blood biochemical parameters of the common carp exposed to the salinity stress.

کلیدواژه‌ها [English]

  • Common carp (Cyprinus carpio)
  • Thyme extract
  • Resistance
  • Blood biochemical parameters
  • Salinity stress
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