بررسی تنوع لوکوس های صفات کمی (QTLs) مرتبط با صفت رشد در بچه ماهیان قزل آلای رنگین کمان پرورشی

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 استادیار پژوهشی مرکز تحقیقات ژنتیک و اصلاح نژاد ماهیان سردآبی شهید مطهری یاسوج، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، یاسوج، ایران

2 دانشیار دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

جهت اجرای این تحقیق از 30 مولد نر و ماده قزل آلای رنگین کمان استفاده شد. پس از پرورش به مدت شش ماه، استخراج ژنوم از بچه ماهیان صورت پذیرفت. تعداد 4 جفت لوکوس صفت کمی (QTL) در این تحقیق مورد استفاده قرار گرفت. آنالیزهای مولکولی نشانگرهای QTL نظیر پیوستگی صفت-نشانگر (آماره LD) به کمک نرم افزار MapChart 2.1 انجام پذیرفت. آنالیز ارتباط بین ژنوتیپ ها و صفت رشد با استفاده از مدل خطی GLMبه کمک نرم افزار SPSS 21.0 انجام شد. با توجه به نتایج این بررسی هتروزیگوسیتی مشاهده شده (Ho) و مورد انتظار (He) در جایگاههای QTL به ترتیب بین 699/0 - 354/0 مربوط به جایگاه OMM5140 و 836/0 - 568/0 مربوط به جایگاه OMM1268 بدست آمدند. میزان درصد واریانس (PV) نیز برای صفت وزن بدن در سن 30 و 180 روزگی به ترتیب 48/18 و 24/31 محاسبه شد. همچنین در بررسی حاضر اثر اپیستازی معنی داری بین جایگاههای QTL مورد مطالعه، مشاهده نگردید. در بررسی تعادل هاردی- واینبرگ نیز در بیشتر جایگاههای مورد بررسی انحراف از تعادل هاردی– واینبرگ را مشاهده شد (p <0.05). با توجه به تنوع جایگاه ژنی کنترل کننده صفات کمی نمونه های قزل آلای رنگین کمان و از طرفی اهمیت شناسایی لوکوس های صفات کمی مرتبط با رشد، لوکوس های صفات کمی مرتبط با صفت مزبور در خانواده های ماهی قزل آلای رنگین کمان را می توان در برنامه های آینده و یا مزارع پرورشی مختلف مورد استفاده قرار داد.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation of quantitative trait loci (QTL) variations for growth-related traits in farmed rainbow trout (Oncorhynchus mykiss)

نویسندگان [English]

  • Sajad Nazari 1
  • Hamed Paknejad 2
1 1Assistant Prof., Shahid Motahary Cold-Water Fishes Genetic and Breeding Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Yasouj, Iran
2 2Associate Prof., Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

In this study thirty male and female broodstock of rainbow trout (n=30) for generating full sibling collected in Cold-water Fishes Genetic and Breeding Research Center. We generated 13 different families using factorial mating design. Then juveniles were raised at the ponds until 6 months post-hatching. Genome extracted based on standard method and these individuals randomly selected for molecular analysis. Fin clips were cut and specimens were kept at -20̊ C until use. Four specific primers were used for rainbow trout and all the four QTL loci screened in this study were successfully amplified in all families. Statistical analyses including linkage disequilibrium (LD), association between genotypes and two quantitative traits including body weight (BW) and total length (TL) were performed using MapChart 2.1, GDA 1.1. General Linear Model (GLM) was performed with software SPSS 21.0. The results demonstrated the mean observed heterozygosity (Ho) and expected heterozygosity (HE) varied between 0.354 to 0.699 for locus OMM5140 and 0.568 to 0.836 for locus OMM1268, respectively. No significant epistatic interactions were identified between QTL markers. Proportion of phenotypic variation explained by each QTL (PV) for body weight at age 30 and 180 were 18.48 and 31.24, respectively. Hardy-Weinberg departure was observed for most loci from all farms and were disequilibrium (p < 0.05). The four QTL loci variation in rainbow trout is important to gain a better understanding of the genetics of production traits and for transferring genetic information and improved selective breeding program to farms in Iran

کلیدواژه‌ها [English]

  • Rainbow trout
  • QTL
  • marker assisted selection
  • selection

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مجله بهره برداری و پرورش آبزیان
دوره 9، شماره 2
تیر 1399
صفحه 95-104

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