بهینه‌سازی کشت نانوکلروپسیس اوکولاتا: اثرات هم‌افزای تنش شوری و فراصوت برای تولید زیست‌توده پایدار

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران

2 نویسنده مسئول، گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران

3 گروه مهندسی ماشین‌های کشاورزی، دانشکده کشاورزی، دانشگاه تهران، کرج، ایران

4 گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران.

5 گروه مهندسی ماشین‌های کشاورزی، دانشکده کشاورزی، دانشگاه تهران، کرج، ایران.

چکیده

در این تحقیق، بهینه‌سازی کشت نانوکلروپسیس اوکولاتا به‌عنوان یک میکروجلبک فتوسنتزی با هدف افزایش تولید زیست‌توده مورد بررسی قرار گرفت. در این مطالعه از تنش شوری (20 ppt) و استفاده از فناوری فراصوت برای بهبود کارایی رشد زیست‌توده استفاده شد. آزمایش‌ها تحت شرایط بهینه کنترل‌شده در آزمایشگاه فیکولوژی انجام شد، که شامل دمای 24 درجه سانتی‌گراد، نور 5000 لوکس، pH معادل 8 و شوری 32 درصد بود. برای اندازه‌گیری رشد میکروجلبک‌ها، از معیارهای مختلفی مانند چگالی نوری (در 750 نانومتر)، کلروفیل (در 650 و 665 نانومتر)، کاروتنوئیدها (در 470 نانومتر) و تعداد سلول‌ها استفاده شد. نتایج نشان داد که ترکیب فراصوت و شوری می‌تواند به‌طور قابل‌توجهی رشد زیست‌توده و میزان رنگدانه‌ها را افزایش دهد. نتایج تحلیل‌های آماری مانند ANOVA و Tukey HSD نشان دادند که تیمارهای تحت تاثیر فراصوت بهترین نتایج را در مقایسه با تیمار شاهد داشتند. به‌طور میانگین، تیمار 7 بالاترین میزان کاروتنوئید را به میزان 992/1 داشت، که در مقایسه با 924/0در تیمار شاهد افزایش چشمگیری نشان داد. تیمار 9 بیشترین سطح تولید کلروفیل در 665 نانومتر را با مقدار 998/1 به دست آورد، در حالی که در تیمار شاهد این مقدار 894/0 بود. همچنین، تیمار 24 بالاترین مقدار کلروفیل در 650 نانومتر را با مقدار 014/1 ثبت کرد که در مقایسه با 724/0 در تیمار شاهد برتری چشمگیری داشت. تیمار 17 نیز بیشترین چگالی نوری را با مقدار 272/1 در 750 نانومتر به دست آورد، که در مقایسه با 77/0 در تیمار شاهد قابل‌توجه بود. این نتایج نشان‌دهنده اثر مثبت فراصوت بر رشد نانوکلروپسیس اوکولاتا بود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Optimization of Nannochloropsis oculata Cultivation: Synergistic Effects of Salinity Stress and Ultrasound for Sustainable Biomass Production

نویسندگان [English]

  • Fariba Akbarzadeh Kargan 1
  • Mohammad Ali Nematollahi 2
  • Mahmoud Soltani Firouz 3
  • Seyed Vali Hosseini 4
  • Pouya Farshbaf Aghajani 5
1 Dept. of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
2 Corresponding Author, Dept. of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
3 Dept. of Agricultural Machinery Engineering, Faculty of Agriculture, University of Tehran, Karaj, Iran.
4 Dept. of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
5 Dept. of Agricultural Machinery Engineering, Faculty of Agriculture, University of Tehran, Karaj, Iran
چکیده [English]

This study investigated the optimization of Nannochloropsis oculata cultivation, a photosynthetic microalga, to enhance biomass production. The aim was to address global challenges in food security, energy, and environmental sustainability through a two-stage cultivation strategy. This strategy utilized salinity stress (20 ppt) and ultrasound technology to improve biomass growth efficiency. Experiments were conducted under controlled laboratory conditions in a psychology lab, with optimal parameters including a temperature of 24°C, light intensity of 5000 lux, pH of 8, and salinity of 32%. The experiments were performed in 1-liter flasks under controlled conditions to evaluate the effects of ultrasound and salinity on biomass growth. Various metrics, such as optical density (OD at 750 nm), chlorophyll (at 650 and 665 nm), carotenoids (at 470 nm), and cell counts, were used to measure microalgal growth. The results demonstrated that the combination of ultrasound and salinity significantly enhanced biomass growth and pigment content. Statistical analyses, including ANOVA and Tukey HSD, revealed that ultrasound-treated samples outperformed the control group. On average, Treatment 7 exhibited the highest carotenoid content at 1.992, a significant increase compared to 0.924 in the control. Treatment 9 achieved the highest chlorophyll content at 665 nm with a value of 1.998, compared to 0.894 in the control. Additionally, Treatment 24 recorded the highest chlorophyll content at 650 nm with a value of 1.014, compared to 0.724 in the control. Treatment 17 showed the highest optical density at 750 nm with a value of 1.272, compared to 0.77 in the control. These results highlight the positive effects of ultrasound and salinity on the growth of Nannochloropsis oculata and the enhancement of biomass production under optimal conditions. Overall, treatments combining ultrasound and high salinity, particularly Treatments 7, 9, 24, and 17, demonstrated superior performance compared to the control and were identified as the optimal cultivation conditions for biomass production.

کلیدواژه‌ها [English]

  • Microalgae
  • environmental sustainability
  • food security
  • photobioreactor
  • statistical optimization

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مجله بهره برداری و پرورش آبزیان
دوره 14، شماره 4
دی 1404
صفحه 83-100

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