نوع مقاله : مقاله کامل علمی - پژوهشی
نویسندگان
1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران
2 دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران
3 گروه مهندسی ماشینهای کشاورزی، دانشکده کشاورزی، دانشگاه تهران، کرج، ایران.
4 گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران.
5 دانشکده کشاورزی، دانشگاه تهران، کرج، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
This study investigated the optimization of Nannochloropsis oculata cultivation, a photosynthetic microalga, to enhance biomass production. The aim was to address global challenges in food security, energy, and environmental sustainability through a two-stage cultivation strategy. This strategy utilized salinity stress (20 ppt) and ultrasound technology to improve biomass growth efficiency. Experiments were conducted under controlled laboratory conditions in a psychology lab, with optimal parameters including a temperature of 24°C, light intensity of 5000 lux, pH of 8, and salinity of 32%. The experiments were performed in 1-liter flasks under controlled conditions to evaluate the effects of ultrasound and salinity on biomass growth. Various metrics, such as optical density (OD at 750 nm), chlorophyll (at 650 and 665 nm), carotenoids (at 470 nm), and cell counts, were used to measure microalgal growth. The results demonstrated that the combination of ultrasound and salinity significantly enhanced biomass growth and pigment content. Statistical analyses, including ANOVA and Tukey HSD, revealed that ultrasound-treated samples outperformed the control group. On average, Treatment 7 exhibited the highest carotenoid content at 1.992, a significant increase compared to 0.924 in the control. Treatment 9 achieved the highest chlorophyll content at 665 nm with a value of 1.998, compared to 0.894 in the control. Additionally, Treatment 24 recorded the highest chlorophyll content at 650 nm with a value of 1.014, compared to 0.724 in the control. Treatment 17 showed the highest optical density at 750 nm with a value of 1.272, compared to 0.77 in the control. These results highlight the positive effects of ultrasound and salinity on the growth of Nannochloropsis oculata and the enhancement of biomass production under optimal conditions. Overall, treatments combining ultrasound and high salinity, particularly Treatments 7, 9, 24, and 17, demonstrated superior performance compared to the control and were identified as the optimal cultivation conditions for biomass production.
کلیدواژهها [English]
)