نوع مقاله : مقاله کامل علمی - پژوهشی
نویسندگان
1 پژوهشکده اکولوژی دریای خزر، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، ساری
2 پژوهشکده اکولوژی دریای خزر، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، ساری، ایران.
3 موسسه تحقیقات علوم شیلاتی کشور
4 دانشگاه علوم کشاورزی و منابع طبیعی گرگان
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Considering the importance of using commercial species of spirulina microalgae, the purpose of this research is to identify the native species of spirulina of the Caspian Sea and to optimize the growth conditions of this microalgae in order to reduce costs and increase the benefits of its production. In this research, water sampling was done from the depth of 5 meters of Farah Abad-Mazandaran beach in 2023-2024. After observing the genus Spirulina, the isolation and purification of its microalgae was done using the dilution method, and finally, pure single colonies were obtained. Initial identification was done using optical microscope and valid identification keys. Molecular identification and sequencing of 16 SrRNAs were used to confirm its authenticity. The results of drawing the phylogenetic tree showed that the studied species has the lowest genetic distance with the microalgae Spirulina laxissima. After identifying the investigated cyanobacterial species, the effect of 3 physical factors of temperature (27-29°C), light (3000-3500 lux) and pH (9-11) on its biomass production using surface response method checked. The results obtained from the design of this experiment showed that after 14 days, the highest biomass (0.839 grams) was obtained at a temperature of 29 degrees, light of 3250 lux and pH=10.
کلیدواژه‌ها [English]
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