اثرات به‌کارگیری مجزا و تلفیقی پودر گشنیز و لاکتوباسیلوس پلانتاروم بر عملکرد رشد، پارامترهای ایمنی سرم و موکوس در ماهی گورخری(Danio rerio)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

2 نویسنده مسئول، گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

4 گروه شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

چکیده

این تحقیق به‌منظور بررسی اثرات به‌کارگیری مجزا و تلفیقی پودر گشنیز و لاکتوباسیلوس پلانتروم بر عملکرد رشد، پارامترهای ایمنی سرم و موکوس در ماهی گورخری(Danio rerio) انجام شد. بدین منظور تعداد 360 قطعه ماهی گورخری با میانگین وزن حدود 3/0گرم تهیه و به‌مدت دو هفته برای سازگاری با شرایط آزمایش نگهداری شد. تغذیه با 4 تیمار و هر تیمار با سه تکرار شامل گروه شاهد ( غذای تجاری بدون افزودنی)، غذای تجاری همراه با 1% پودر گشنیز، گروه سوم تغذیه شده با غذای تجاری همراه با CFU/g 107 پروبیوتیک لاکتوباسیلوس پلانتاروم و گروه چهارم تغذیه با غذای تجاری همراه با 1% پودر گشنیز و CFU/g 107پروبیوتیک لاکتوباسیلوس پلانتاروم می باشد. در پایان دوره از تیمارها نمونه‌برداری شد. نتایج شاخص های رشد نشان داد اختلاف معنی داری در شاخص‌های وزن اولیه و درصد بقا در هیچ یک از تیمارهای مجزای پودرگشنیز و لاکتوباسیلوس پلانتاروم و تیمار ترکیبی ( با اثر متقابل) نشان نداد (P>0.05) . در تیمار وزن نهایی، افزایش وزن و درصد افزایش وزن اختلاف معنی داری در تیمار مجزای گشنیز مشاهده نشد (P>0.05) ولی تیمارهای لاکتوباسیلوس پلانتاروم و ترکیبی با اثر متقابل اختلاف معنی داری را با سایر تیمارها نشان دادند (P≤0.05). میزان پروتئین کل سرم اختلاف معنی داری در تیمار مجزای پروبیوتیک و ترکیبی با سایر تیمارها مشاهده شد (P≤0.05) همچنین تیمار حاوی پودر گشنیز اختلاف معنی داری را با تیمار شاهد نشان داد (P≤0.05). در میزان لایزوزیم سرم در تیمارهای پودر گیشنیز، لاکتوباسیلوس پلانتاروم و تیمار ترکیبی نسبت به گروه شاهد اختلاف معنی‌داری را نشان دادند (P≤0.05).

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of combined or singular administration of Coriandrum sativum and Lactobacillus plantarumi on growth performance, serum and mucus immunity parameters in Zebrafish (Danio rerio)

نویسندگان [English]

  • Iman Sabori Gorgan 1
  • Valiollah Jafari 2
  • Roghayeh Safari 3
  • Ali Hajibeglou 4
1 M.Sc. Student of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Corresponding Author, Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
3 Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
4 Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
چکیده [English]

This study was performed to investigate the effects of separate and combined application of coriander powder and Lactobacillus plantrum on growth performance, immune parameters of serum and mucus in zebrafish (Danio rerio). For this purpose, 360 pieces of zebrafish with an average weight of about 0.3 g were prepared and kept for two weeks to adapt to the experimental conditions. Feeding with 4 treatments and each treatment with three replications including control group (commercial food without additives), commercial food with 1% coriander powder, the third group fed with commercial food with 107 CFU / g Lactobacillus plantarum probiotic and the fourth group fed with Commercial food with 1% coriander powder and 107 CFU / g probiotic Lactobacillus plantarum. At the end of the course, the treatments were sampled. The results of growth indices showed no significant difference in initial weight indices and survival percentage in any of the separate treatments of powdery mildew and Lactobacillus plantarum and combined treatment (with interaction) (P> 0.05). There was no significant difference in final weight treatment, weight gain and weight gain percentage in separate coriander treatment (P> 0.05) but Lactobacillus plantarum and combination treatments showed significant differences with other treatments (P≤0.05). Total serum protein content was significantly different in separate probiotic and combination treatments with other treatments (P≤0.05) the treatment containing coriander powder showed a significant difference with the control treatment (P≤0.05). Serum lysozyme levels in coriander powder treatments, Lactobacillus plantarum and combination treatment showed a significant difference compared to the control group (P≤0.05). Total serum immunoglobulin level showed a significant difference in the separate treatment of probiotics and coriander with control treatment (P≤0.05) the combined treatment showed a significant difference with other treatments (P≤0.05). The amount of total mucus protein did not show in any of the separate treatments of powdery mildew and Lactobacillus plantarum and combination treatment (with interaction) (P> 0.05). There was a significant difference in the amount of mucus lysozyme in the combined treatment with other treatments (P≤0.05), the treatment containing probiotics showed a significant difference with the control and coriander (P≤0.05). There was a significant difference in the amount of total mucus immunoglobulin in the combined treatment with other treatments (P≤0.05) the treatment containing coriander powder and separate probiotics showed a significant difference with the control treatment (P≤0.05). In general, it can be said that the use of probiotics Lactobacillus plantarum and coriander powder improves the condition of Zebrafish.

کلیدواژه‌ها [English]

  • Probiotics
  • Coriander powder
  • Zebrafish
  • Safety

مراجع

1.Bondad-Reantaso, M. G., Subasinghe, R. P., Arthur, J. R., Ogawa, K., Chinabut, S., Adlard, R., Tan, Z., & Shariff, M. (2005). Disease and health management in Asian aquaculture. Veterinary Parasitology. 132, 249-272.
2.Hoseinifar, S. H., Khalili, M., Rostami, H. K., & Esteban, M. Á. (2013). Dietary galactooligosaccharide affects intestinal microbiota, stress resistance, and performance of caspian roach (Rutilus rutilus) fry. Fish & Shellfish Immunology. 35, 1416-1420.
3.Arasu, M. V., Jung, M. W., Ilavenil, S., Jane, M., Kim, D. H., Lee, K. D., Park, H. S., Hur, T. Y., Choi, G. J., Lim, Y. C., Al-Dhabi, N. A., & Choi, K. C. (2013). Isolation and characterization of antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties. J. Appl. Microbiol. 115, 1172-1185.
4.Safari, R., Hoseinifar, S. H., Dadar, M., Sattari, M., & Rahbar, M. (2019). The effects of Coriandrum sativum L. as
feed additive on mucosal immune parameters, antioxidant defence and, immune-related genes expression in zebrafish (Danio rerio). Aquaculture Research. 50 (9), 2621-2627.
5.Safari, R., Vahedi Amiri, F., Shabany, A., Hoseinifar, S. H., & Kolangi Miandareh, H. (2017). Effect of Supplementation of Diet with Ferula assafoetida Hydoalkohalic Extract on Immune Related (IL-1β and TNF-alpha) Gene. Physiology and animal development (biological sciences). 11 (3), 55-66.
6.Nezhadmoghadam, S., Imanpoor, M. R., Jafari., V., & Safari, R. (2018). Effect of Achillea millefolium on mucosal immune response and immune related (TNF-alfa) gene expression in Goldfish Carassius auratus (Linnaeus, 1758). JAIR.
5 (4), 87-100.
7.Hoseinifar, S. H., Mirvaghefi, A. R., Mojazi Amiri, B., Khoshbavar Rostami, H. A., Poor Amini, M., & Darvish Bastami, K. (2011). The probiotic effects of dietary inactive yeast Saccharomyces cerevisiae var. ellipsoideus on growth factors, survival, body composition and intestinal microbiota of juvenile Beluga (Huso huso). Iranian Scientific Fisheries Journal. 19 (4), 55-66.
8.Darvishi, M., Safari, R., Shabani, A., & Hoseinifar, S. H. (2020). Effects of sublethal concentration of diazinon on the expression of aromatase (CYP 19a) gene in females Zebrafish (Danio rerio). Journal of Animal Enviroment. 12 (3), 395-400.
9.Misra, C. K., Das, B. K., Mukherjee, S. C., & Pattnaik, P. (2006). Effect of multiple injections of β-glucan on non-specific immunoresponse and disease resistance in Labeo rohita fingerlings. Fish & Shellfish Immunology. 20 (3), 305-331.
10.Ross, T., Dalgaard, P., & Tienungoon, S. (2000). Predictive modelling of the growth and survival of Listeria in fishery products. International Journal of Food Microbiology. 62 (3), 231-245.
11.Ellis, A. E. (1990). Lysozyme Assays. In: Stolen, J. S., Fletcher, T. C., Anderson, D. P., Roberson, B. S. and Van Muiswinkel, W. B., Eds., Techniques in Fish Immunology Fair Haven, SOS Publications, Fair Haven, 101-103.
12.Anderson, W. G., McKinley, R. S., & Colavecchia, M. (1997). The use of clove oil as an anaesthetic for rainbow trout and its effect on swimming performance. North American Journal of Fisheries Management. 17, 301-307.
13.Wang, Y. B., Li, J. R., & Lin, J. (2008). Probiotics in aquaculture: Challenges and outlook. Aquaculture. 281, 1-4.
14.Ferguson, R. M. W., Merrifield, D. L., Harper, G. M., Rawling, M. D., Mustafa1, S., Picchietti, S., Balcazar, J. L., & Davies, S. J. (2010). The effect of Pediococcus acidilactici on the gut microbiota and immune status of ongrowing red tilapia (Oreochromis niloticus). Journal of Applied Microbiology. 109 (3), 1364-5072.
15.Tackert, W., Abelin, P., & Sorgeloos, P. (1989). Stress resistance in postlarval penaeid shrimp reared under different feeding procedure. Journal of World Aquaculture Society. 20: 74A.
16.Srinivasan, K. (2016). Spices and Flavoring Crops: Uses and Health Effects, In: Encyclopedia of Food and Health. Academic Press, Oxford, pp. 98-105.
17.Citarasu, T. (2010). Herbal biomedicines: a new opportunity for aquaculture industry. Aquac. Int. 18, 403-414.
18.Begnami, A. F., Spindola, H. M., Ruiz, A. L., de Carvalho, J. E., Groppo, F. C., & Rehder, V. L. (2018). Antinociceptive and anti-edema properties of the ethyl acetate fraction obtained from extracts of Coriandrum sativum Linn. Leaves, Biomed. Pharmacother. 103, 1617-22.
19.Rezvani Gilkolaei, A., Shoaibi Omrani, B., & Afraei Bandpey, M. A. (2019). The effect of dietary probiotic Lactobacillus plantarum on nutrition performance in Siberian sturgeon (Acipenser baerii) (Huso huso). JAD. 13 (1), 79-88.
20.Hosseini, A., Chaharlang, F., Sotoudeh, E., Alishahi, M., & Modaresi, M. (2016). The effect of isolated Lactobacillus from gut of Barbus grypus on growth performance, survival and gut microflora of common carp (Cyprinus carpio). ISFJ. 25 (3), 167-179.
21.Farsani, M. N., Hoseinifar, S. H., Rashidian, G., Farsani, H. G., Ashouri, G., & Van Doan, H. (2019). Dietary effects of Coriandrum sativum extract on growth performance, physiological and innate immune responses and resistance of rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Fish and Shellfish Immunology. 91, 233-240.
22.Ghaljaei Fard, A., Khara, H., & Shenavar Masoleh, A. (2016). Effect of Lactobacillus plantarum (KC426951) Bacteria Isolated from the Intestine of Rainbow Trout Guilan on Hematological Indices and Immune of Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) Fry. Journal of Physiology and animal development (biological sciences). 9 (1), 61-68.
23.Pourgholam, M. A., Khara, H., Safari, R., Yazdani Sadati, M. A., & Aramli, M. S. (2017). Influence of Lactobacillus plantarum Inclusion in the Diet of Siberian Sturgeon (Acipenser baerii) on Performance and Hematological Parameters. Turkish Journal of Fisheries and Aquatic Sciences. 17, 1-5.
24.Xavier Innocent, B., Syed Ali fathima, M., & Dhanalakshmi, A. (2011). Studies on the immunostimulant activity of Coriandrum sativum and resistance to Aeromonas hydrophila in Catla catla. Journal of Applied Pharmaceutical Science. 1 (07), 132-135.
25.Tuan, T. N., Duc, P. M., & Hatai, K. (2013). Overview of the use of probiotics in aquaculture. Int. J. Res. Fish Aquac. 3, 89-97.
26.Saurabh, S., & Sahoo, P. (2008). Lysozyme: an important defence molecule of fish innate immune system. Aquac. Res. 39, 223-239.
27.Pimpimol, T., Phoonsamran, K., & Chitmanat, C. (2012). Effect of dietary vitamin C supplementation on the blood parameters of Mekong giant catfish (Pangasianodon gigas). Int. J. Agric. Biol. 14, 256-260.
28.Jatob´a, A., Vieira, Fd. N., Buglione-Neto, C. C., Mouri˜no’, J. L. P., Silva, B. C., Seiftter, W. Q., & Andreatta, E. R. (2011). Diet supplemented with probiotic for Nile tilapia in polyculture system with marine shrimp. Fish Physiol. Biochem. 37, 725-732.
29.Zhang, X., Huang, K., Zhong, H., Ma, Y., Guo, Z., Tang, Z., Liang, J., Luo, Y., Su, Z., & Wang, L. (2020). Effects of Lycium barbarum polysaccharides on immunological parameters, apoptosis, and growth performance of Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol. 97, 509-514.
30.Pu, H., Li, X., Du, Q., Cui, H., & Xu, Y. (2017). Research Progress in the Application of Chinese Herbal Medicines in Aquaculture: A Review. Engineering. 3, 737-731.
31.Ishida, M., Nishi, K., Kunihiro, N., Onda, H., Nishimoto, S., & Sugahara, T. (2017). Immunostimulatory effect of aqueous extract of Coriandrum sativum L. seed on macrophages. J. Sci. Food Agric. 97, 4727-4736.
32.Mohammadiyan, T., Bita, S., & Naseri Pourtaklo, R. Effect of Lactobacillus plantarum Encapsulated with Alginate / Chitosan on Biochemical Factors in the Beluga (Huso huso). Journal of Veterinary Research. 74 (1), 93-103.
33.Hamedi, S., Rahimi, R., Nafisi Bahabadi, M., Azodi, M., & Ahmadi, S. A. (2020). Study of Some Liver Enzymes Changes (Lates calcarifer) at Different Levels of Water Salinity. Physiology and animal development (biological sciences). 12 (4), 61-74.
مجله بهره برداری و پرورش آبزیان
دوره 13، شماره 3
مهر 1403
صفحه 167-182

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