نوع مقاله : مقاله کامل علمی - پژوهشی
نویسندگان
تکثیر و پرورش آبزیان، دانشکده شیلات محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
This study was performed to investigate the effects of separate and combined application of coriander powder and Lactobacillus plantrum on growth performance, immune parameters of serum and mucus in zebrafish (Danio rerio). For this purpose, 360 pieces of zebrafish with an average weight of about 0.3 g were prepared and kept for two weeks to adapt to the experimental conditions. Feeding with 4 treatments and each treatment with three replications including control group (commercial food without additives), commercial food with 1% coriander powder, the third group fed with commercial food with 107 CFU / g Lactobacillus plantarum probiotic and the fourth group fed with Commercial food with 1% coriander powder and 107 CFU / g probiotic Lactobacillus plantarum. At the end of the course, the treatments were sampled. The results of growth indices showed no significant difference in initial weight indices and survival percentage in any of the separate treatments of powdery mildew and Lactobacillus plantarum and combined treatment (with interaction) (P> 0.05). There was no significant difference in final weight treatment, weight gain and weight gain percentage in separate coriander treatment (P> 0.05) but Lactobacillus plantarum and combination treatments showed significant differences with other treatments (P≤0.05). Total serum protein content was significantly different in separate probiotic and combination treatments with other treatments (P≤0.05) the treatment containing coriander powder showed a significant difference with the control treatment (P≤0.05). Serum lysozyme levels in coriander powder treatments, Lactobacillus plantarum and combination treatment showed a significant difference compared to the control group (P≤0.05). Total serum immunoglobulin level showed a significant difference in the separate treatment of probiotics and coriander with control treatment (P≤0.05) the combined treatment showed a significant difference with other treatments (P≤0.05). The amount of total mucus protein did not show in any of the separate treatments of powdery mildew and Lactobacillus plantarum and combination treatment (with interaction) (P> 0.05). There was a significant difference in the amount of mucus lysozyme in the combined treatment with other treatments (P≤0.05), the treatment containing probiotics showed a significant difference with the control and coriander (P≤0.05). There was a significant difference in the amount of total mucus immunoglobulin in the combined treatment with other treatments (P≤0.05) the treatment containing coriander powder and separate probiotics showed a significant difference with the control treatment (P≤0.05). In general, it can be said that the use of probiotics Lactobacillus plantarum and coriander powder improves the condition of Zebrafish.
کلیدواژهها [English]
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