نوع مقاله : مقاله کامل علمی - پژوهشی
نویسندگان
1 عضو هیئت علمی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان
2 گروه شیلات، دانشگاه آزاد اسلامی واحد آزادشهر، آزادشهر، ایران.
3 گروه شیلات و حفاظت آب، دانشکده سوث بوهمیا، جمهوری چک
4 گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.
5 گروه علوم و فنون دریا، دانشگاه پلی تکنیک اسکندرون، ترکیه
6 گروه شیلات و علوم دامی، دانشکده کشاورزی، دانشگاه چیانگ مای، تایلند
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
The aim of this research is to investigate the effects of different levels of dietary fulvic acid on systemic immunity, antioxidant capacity, and immune-antioxidant relative gene expression in zebrafish (Danio rerio). In order to investigate the effect of dietary fulvic acid (FLA), 600 healthy fish (average body weight 96.01 ± 3.01 mg) were randomly placed in 12 aquariums. During an experimental period of 8 weeks, fish was fed basal diets containing different levels of fulvic acid (0 (ctrl), 0.25 (FLA1), 0.5 (FLA2) and 1 (FLA3) g/kg diet) and they were fed three times a day. At the end of the experimental period, no significant difference was observed among experimental treatments regarding growth performance (P < 0.05). However, lysozyme activity, total immunoglobulin (Ig) and total protein of whole body extract were significantly increased in response to 0.5-1 g kg-1 diet fulvic acid compared to other treatments (P<0.05). However, no difference was observed among the experimental groups in terms of catalase, glutathione peroxidase and superoxide dismutase enzyme activities. The interferon-α (INF-α) and tumor necrosis factor alpha (TNF-α) genes expression increased in the treatments using fulvic acid, and the highest level was observed in 0.5 g/kg fulvic acid received treatment (P < 0.05). Also, the expression of interleukin 1 (IL-1) gene in this group was significantly increased compared to other experimental treatments (P<0.05). The expression of GPx gene was significantly increased in the group received fulvic acid without any significant difference among the treatments (P>0.05). CAT gene expression did not show any difference among experimental groups (P>0.05). Although SOD gene expression showed a significant increase in response to dietary fulvic acid, which the highest level was observed in the 0.5 g/kg received group (P < 0.05). In conclusion, according to the positive results obtained, fulvic acid can be potentially used as a feed additive in Zebrafish to boost growth performance and immnene performance and regulate anti-oxidant, and immune gene expression.
کلیدواژه‌ها [English]
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