کاهش استرس طی حمل و نقل بچه ماهی کپور دریایی(Cyprinus carpio) با استفاده از سطوح مختلف نمک سدیم کلرید

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد تکثیر و پرورش آبزیان، دانشکده شیلات و محیط‌زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

2 نویسنده مسئول، استاد گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط‌زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 دانشیار گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط‌زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

4 دانشجوی دکتری بیوتکنولوژی آبزیان، دانشکده شیلات و محیط‌زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

چکیده

برای این پژوهش تعداد 240 قطعه بچه‌ماهی کپور معمولی (با میانگین وزن 1± 5گرم) در 4 تیمار مختلف شوری 0، 1،3 و 6 گرم در لیتر در سه تکرار (در کیسه‌های پلاستیکی) به مدت زمان یک ساعت حمل شد. جهت بررسی پارامترهای خونی بچه ماهیان در پایان آزمایش، از نمونه‌ها به روش قطع ساقه دمی خون‌گیری صورت گرفت. طبق نتایج حاصل از اندازه‌گیری برخی پارامترهای بیوشیمیایی سرم خون، میزان پروتئین‌کل در بین تیمارهای مختلف اختلاف معنی‌داری را نشان نداد(P>0.05) ولی میزان گلوکز و کورتیزول در تیمارهای شوری کاهش معنی‌داری را نسبت به شاهد نشان دادند(P≤0.05). نتایج حاصل از اندازه‌گیری آنزیم‌های کبدی در میزان آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز و آلکالین فسفاتاز بین تیمارهای مختلف اختلاف معنی‌داری را نشان نداد(P>0.05). نتایج حاصل از اندازه‌گیری برخی فاکتورهای هماتولوژی خون نیز حاکی از عدم اختلاف معنی‌دار در تعداد گلبول‌های قرمز و سفید، درصد هماتوکریت و میزان هموگلوبولین در تیمارها بود(P>0.05). به‌طور کلی می‌توان گفت در ماهیان حمل شده در تیمار 3 گرم نمک در لیتر شاخص‌های استرس کمتر مشاهده شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Reducing stress during transportation of sea carp fry (Cyprinus carpio) using different levels of sodium chloride salt

نویسندگان [English]

  • Bahman Iri 1
  • Mohamadreza imanpour 2
  • Seyed Hossein hosseinifar 3
  • Roghayeh Safari 3
  • Elahe Rohani 4
1 M.Sc. Student of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Corresponding Author, Professor, Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
3 Associate Prof., Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
4 Ph.D. Student of Aquatic Biotechnology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
چکیده [English]

For this research, 240 pieces of sea carp fry (with an average weight of 5±1g) were transported in 4 different salinity treatments of 0, 1, 3 and 6 g/l in three replicates (in plastic bags) for one hour. In order to check the blood parameters of the baby fish at the end of the experiment, blood was taken from the samples by cutting the tail stem. According to the results of measuring some biochemical parameters of blood serum, the amount of total protein did not show a significant difference between different treatments (P>0.05), but the amount of glucose and cortisol showed a significant decrease in saline treatments compared to the control (P<0.05). The results of measuring liver enzymes in the amount of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase showed no significant difference between different treatments (P>0.05). The results of measuring some blood hematology factors also indicated no significant difference in the number of red and white blood cells, hematocrit percentage and hemoglobin level in the treatments. (P>0.05). In general, it can be said that Fewer stress indicators were observed in the transported fish treated with 3 grams of salt per liter.

کلیدواژه‌ها [English]

  • table salt
  • transportation
  • common carp
  • biochemical parameters
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