بهینه‌سازی استخراج پروتئین‌های تشکیل دهنده ژل و کلاژن هیدرولیز شده از فانوس ماهی گونه Benthosema pterotum با یک رویکرد بیوریفاینری

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 فرآوری محصولات شیلاتی، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

2 نویسنده مسئول، گروه شیلات، دانشکدة منابع طبیعی دانشگاه تهران ، کرج، ایران، دانشیار گروه فرآوری محصولات شیلاتی، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 گروه بیولوژی و مهندسی زیستی- تغذیه و علوم غذایی، دانشگاه صنعتی چالمرز، سوئد.

4 گروه فرآوری محصولات شیلاتی، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

چکیده

در این کار از مزایای استفاده همزمان از رویکرد بیوریفاینری و بهینه‌سازی برای دست‌یابی به کارایی فرآیند کل مطلوب استفاده شد. ابتدا از فرآیند تغییر pH قلیایی کمک شده با التراسونیک و بهینه سازی آن به منظور کمک به بیشینه کردن درصد بازدهی استخراج و شاخص سفیدی پروتئین‌های عضله فانوس ماهی گونه B. pterotum استفاده شد. استراتژی بهینه انتخاب شده با مشخصات بدین شرح؛ نسبت حلال به جامد ml g-1 6، pH انحلال 5/11، توان التراسونیک W 310 ؛ و pH ترسیب 8/5 و با مطلوبیت 84/0؛ بازدهی 53 درصد و شاخص سفیدی 81/76 را ارائه کرد. از این پروتئین در ترکیب با کلاژن هیدرولیز شده در تهیه ژل‌های حرارتی استفاده شد. نتایج نشان داد ژل حاصل از پروتئین ایزوله نیروی شکست و تغییر شکل را به ترتیب در حدود 123 g و 12/10 میلی‌متر ارائه کرد. افزودن کلاژن هیدرولیز شده سبب بهبود نیروی شکست (تا سطح 2% وزنی/وزنی) و مقدار تغییر شکل (تا سطح 10% وزنی/وزنی) شد. کلاژن هیدرولیز شده همچنین تا حدودی بر ویژگی‌های رنگ و شاخص سفیدی و همچنین بر ظرفیت نگهداری آب در ژل‌ها اثر مثبت داشت. بنابراین می‌توان عنوان کرد با اتخاذ یک تکنیک جدید استخراج مثل التراسونیک در کنار فرآیند تغییر pH و متعاقبا بهینه‌سازی آن و همچنین استفاده از رویکرد بیوریفانری به منظور استفاده همزمان از پروتئین‌های عضله و بافت پیوندی موجود در زیست توده ماهی؛ می‌توان گامی مهم در جهت حرکت به سمت scale-up (صنعتی کردن مقیاس تولید)کردن فرآیند برداشت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Extraction optimization of gel-forming proteins and collagen hydrolysate from whole lanternfish (Benthosema pterotum) with a biorefinery approach

نویسندگان [English]

  • Mohsen Kazemi 1
  • Seyed Mahdi Ojagh 2
  • Mehdi Abdollahi 3
  • Alireza Alishahi 4
1 Dept. of Processing of Fishery Products, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Corresponding Author, Associate Prof., Dept of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran ,Dept of Seafood Processing, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Dept. of Life Sciences-Food and Nutrition Science, Chalmers University of Technology, SE 412 96 Gothenburg, Sweden.
4 Dept. of Processing of Fishery Products, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
چکیده [English]

Here the advantages of employing a biorefinery approach in conjunction with optimization techniques were utilized to attain high overall process efficiency. Initially, an alkaline pH-shift process assisted by ultrasonic and its subsequent optimization were employed to maximize the extraction yield and whiteness index of muscle proteins isolated from lanternfish (B. pterotum). The optimal strategy was determined as follows: 6 mL g-1, pH1 (solubilization) 11.5, 310 W, and pH2 (precipitation) 5.8 with desirability of 0.84. Collagen hydrolysate was obtained from the connective tissue-containing sediment residue emerging during pH-shift process. The optimal isolated protein (OIP) was utilized to produce heat-set gels in combination with collagen hydrolysate. Breaking force and deformation of the OIP gel were measured at 123 (g) and 10.12 (mm) respectively. Collagen hydrolysate made breaking force (up to 2% w/w) and deformation (up to 10% w/w) improved and had positive effects on color characteristics (especially whiteness index) as well as water holding capacity. Hence, it can be concluded that the integration of a novel extraction technique such as ultrasonic with pH-shift process followed by optimization, as well as concurrent utilization of muscle and connective tissue proteins through a biorefinery approach; provides a significant advancement towards scaling up the extraction process.

کلیدواژه‌ها [English]

  • Protein
  • pH-shift
  • Ultrasonic
  • Optimization
  • Gel
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