تجویز خوراکی پودر برگ مورینگا (Moringa oleifera) بر برخی پارامترهای خون شناسی و سرمی ماهی قزل آلای رنگین کمان (Oncorhyncus mykiss) قبل و بعد از مواجهه با آئروموناس هیدروفیلا (Aeromonas hydrophila)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 نویسنده مسئول، گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

4 گروه شیلات، دانشگاه آزاد اسلامی، واحد علوم تحقیقات، تهران، ایران

5 گروه علوم آبزیان، پژوهشکده تالاب بین‌المللی هامون، پژوهشگاه زابل، زابل، ایران.

چکیده

جهت بررسی اثر تجویز خوراکی پودر برگ مورینگا تعداد 360 ماهی قزل آلا (5/0 ± 5/35 گرم) به طور تصادفی در 12 مخزن تقسیم شدند. ماهی‌ها با سطوح پودر برگ مورینگا به میزان صفر(شاهد)، 1، 5/2 و 5 درصد به مدت هشت هفته تغذیه شدند. پس از آزمایش چالش با باکتری آئروموناس هیدروفیلا، فعالیت آنزیم‌های آلانین ترانس آمیناز و آسپارتات آمینو ترانسفراز در تیمار 5/2 درصد به طور قابل توجهی نسبت به تیمار شاهد کاهش نشان داد (05/0>P). مقدار بهینه گنجاندن پودر برگ مورینگا در جیره برای آنزیم آسپارتات آمینوترانسفراز بر اساس آنالیز رگرسیون چند جمله ای به ترتیب مقادیر97/1 و 33/3 درصد برای مراحل قبل و بعد از چالش با باکتری بود. تغییر معنی‌داری در مقدار آنزیم آلکالین فسفاتاز، تعداد گلبول‌های قرمز، هموگلوبین و هماتوکریت بین همه تیمارها در مقایسه با تیمار شاهد در هر دو مرحله قبل و بعد از چالش با باکتری مشاهده نشد (05/0<P). مقدار گلوکز در همه تیمارها به ویژه در تیمار 5/2 درصد در مقایسه با تیمار شاهد در هر دو مرحله کاهش معنی داری نشان داد (05/0>P). آنالیز رگرسیون مقدار بهینه مورینگا را برای این فاکتور به ترتیب 40/3 و 77/2 درصد برای قبل و بعد از چالش با باکتری نشان داد. با توجه به نتایج، پودر برگ مورینگا به میزان 5/2 درصد می تواند به کاهش استرس و احتمالا بهبود سلامت عمومی این ماهی کمک کند. دوز مناسب بر اساس مدل رگرسیون چند جمله ای برای پارامترهای بیوشیمیایی سرم مانند گلوکز و آنزیم آسپارتات آمینوترانسفراز از 97/1 تا 4/3 درصد بدست آمد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Oral administration of Moringa oleifera leaf powder on some hematological and serum parameters of rainbow trout (Oncorhyncus mykiss) before and after exposure to Aeromonas hydrophila

نویسندگان [English]

  • Narjes Sanchooli 1
  • Hamed Paknejad 2
  • Mohammad sudagar 3
  • Seyed Pezhman Hosseini Shekarabi 4
  • Abdolali Rahdari 5
1 Dept. of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Corresponding Author, Dept. of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Dept. of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Dept. of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 Department of Aquatic Sciences, Hamoun International Wetland Institute, Research Institute of Zabol, Zabol, Iran.
چکیده [English]

To investigate the effect of oral administration of Moringa leaf powder, 360 rainbow trout (35.5 ± 0.5 g) were randomly divided into 12 tanks. The fish were fed with Moringa leaf powder levels of 0(control), 1, 2.5 and 5% for eight weeks. After the challenge test with Aeromonas hydrophila bacteria, the activity of alanine transaminase and aspartate aminotransferase enzymes in the 2.5% treatment significantly decreased compared to the control treatment (P<0.05). The optimal amount of Moringa leaf powder inclusion in the diet for aspartate aminotransferase enzyme based on polynomial regression analysis was 1.97% and 3.33% respectively for the periods before and after the challenge with bacteria. There was no significant change in the amount of alkaline phosphatase enzyme, the number of red blood cells, hemoglobin and hematocrit between all treatments compared to the control treatment in both periods before and after the challenge with bacteria (P<0.05). The amount of glucose showed a significant decrease in all treatments, especially in the 2.5% treatment compared to the control treatment in both stages (P<0.05). Regression analysis showed the optimal value of Moringa for this factor, 3.40% and 2.77%, respectively, before and after the challenge with bacteria. According to the results, moringa leaf powder at 2.5% can help reduce stress and possibly improve the general health of this fish. The appropriate dose was obtained from 1.97% to 3.4% based on polynomial regression model for serum biochemical parameters such as glucose and aspartate aminotransferase enzyme.

کلیدواژه‌ها [English]

  • Moringa
  • Hematology
  • Blood biochemistry
  • Liver enzymes
  • Aeromonas hydrophila

مراجع

 1.Saeidi Asl, M. R., Adel, M., Caipang, C. M. A., & Dawood, M. A. O. (2017). Immunological responses and disease resistance of rainbow trout (Oncorhynchus mykiss) juveniles following dietary administration of stinging nettle (Urtica dioica). Fish and Shellfish Immunology. 71, 230-238.
2.Paknejad, H., Shekarabi, S. P. H., Mehrgan, M. S., Hajimoradloo, A., Khorshidi, Z., & Rastegari, S. (2020). Dietary peppermint (Mentha piperita) powder affects growth performance, hematological indices, skin mucosal immune parameters, and expression of growth and stress-related genes in Caspian roach (Rutilus caspicus). Fish Physiology Biochemistry. 46 (5), 1883-1895.
3.Li, P., Lewis, D. H., & Galtin, D. M. (2004). Dietary oligonucleotides from yeast RNA influence immune responses and resistance of hybrid striped bass (Morone chrysops Morone saxatilis) to Streptococcus iniae infection. Fish and Shellfish Immunology. 16, 561-569.
4.Faggio, C., Morabito, M., Minicante, S. A., Piano, G. L., Pagano, M., & Genovese, G. (2015). Potential use of polysaccharides from the brown alga Undaria pinnatifida as anticoagulants. Brazilian Archives of Biology and Technology. 58 (5), 798-804.
5.Kumar, J., Priyadharshini, M., Madhavi, M., Begum, S. S., Ali, A. J., Musthafa, M. S., & Faggio, C. (2022). Impact of Hygrophila auriculata supplementary diets on the growth, survival, biochemical and haematological parameters in fingerlings of freshwater fish Cirrhinus mrigala (Hamilton, 1822). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 263, 111097.
6.Van Doan, H., Hoseinifar, S. H., Sringarm, K., Jaturasitha, S., Khamlor, T., Dawood, M. A. O., Esteban, M. Á., Soltani, M., & Musthafa, M. S. (2019). Effects of elephant's foot (Elephantopus scaber) extract on growth performance, immune response, and disease resistance of Nile tilapia (Oreochromis niloticus) fingerlings. Fish and Shellfish Immunology. 93, 328-335.
7.Harikrishnan, R., Balasundaram, C., & Heo, M.S. (2011). Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture. 317 (1-4), 1–15.
8.Lee, J. Y., & Gao, Y. (2012). Review of the application of garlic, Allium sativum, in aquaculture. Journal of the World Aquaculture Society. 43 (4), 447-458.
9.Platel, K., Rao, A., Saraswathi, G., & Srinivasan, K. (2002). Digestive stimulant action of three Indian spice mixes
in experimental rats. Food/Nahrung. 46 (6), 394-398.
10.Mbokane, E. M., & Moyo, N. A. G. (2018). Alterations of haemato-biochemical parameters pre and post-challenge with Aeromonas hydrophila and survival of Oreochromis mossambicus fed Moringa oleifera-based diets. Fish and Shellfish Immunology. doi: 10.1016/j.fsi.2018. 09.017.
11.Foidl, N., Makkar, H. P. S., & Becker, K. (2001). The potential of Moringa oleifera for agricultural and industrial uses. In: L.J. Fuglie, (Ed.). The Miracle Tree. CTA, Wugeningen, Netherlands and CWS, New York, USA, pp: 45-77.
12.Leone, A., Spada, A., Battezzati, A., Schiraldi, A., Aristil, J., & Bertoli, S. (2015). Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. International Journal of Molecular Sciences. 16 (6), 12791-12835.
13.Puycha, K., Yuangsoi, B., Charoenwattanasak, S., Wongmaneeprateep, S., Niamphithak, P., & Wiriyapattanasub, P. (2017). Effect of moringa (Moringa oleifera) leaf supplementation on growth performance and feed utilization of Bocourti's catfish (Pangasius bocourti). Agriculture Natural Resources. 51 (4), 286-291.
14.Kou, X., Li, B., Olayanju, J., Drake, J., & Chen, N. (2018). Nutraceutical or pharmacological potential of Moringa oleifera Lam. Nutrients. 10 (3), 343.
15.Naderi Farsani, M., Hoseinifar, S. H., Rashidian, G., Farsani, H. G., Ashouri, G., & Van Doan, H. (2019). Dietary effects of Coriandrum sativum extract on growth performance, physiological and innate immune responses and resistance of rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Fish and Shellfish Immunology. 91, 233-240.
16.Adel, M., Dawood, M. A. O., Shafiei, S. et al. (2019). Dietary Polygonum minus extract ameliorated the growth performance, humoral immune parameters, immune-related gene expression and resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Aquaculture. 519, 734738.
17.Mostafavi, Z. S., Hosseini Shekarabi, S. P., Shamsaie Mehrgan, M., & Rajabi Islami, H. (2022). Amelioration of growth performance, physio-metabolic responses, and antioxidant defense system in rainbow trout, Oncorhynchus mykiss, using dietary dandelion, Taraxacum officinale, flower extract. Aquaculture International. 546,737296.
18.Elbesthi, R. T. A., Ozdemir, K. Y., Taştan, Y., Bilen, S., & Sonmez, A. Y. (2020). Effects of ribwort plantain (Plantago lanceolata) extract on blood parameters, immune response, antioxidant enzyme activities, and growth performance in rainbow trout (Oncorhynchus mykiss). Fish Physiology Biochemistry. 46 (4), 1295-1307.
19.Moghanlou, K. S., Nasr Isfahani, E., Dorafshan, S., Tukmechi, A., & Aramli, M.S. (2018). Effects of dietary supplementation with Stachys lavandulifolia Vahl extract on growth performance, hemato-biochemical and innate immunity parameters of rainbow trout (Oncorhynchus mykiss). Animal Feed Science and Technology. https:// doi.org/ 10.1016/ j.anifeedsci. 2018. 01. 016.
20.Bilen, S., Abdalsalam, T., Altief, S., Ozdemir, K. Y., Salem, M. O. A., Terzi, E., & Güney, K. (2020). Effect of lemon balm (Melissa officinalis) extract on growth performance, digestive and antioxidant enzyme activities, and immune responses in rainbow trout (Oncorhynchus mykiss). Fish Physiology Biochemistry. 46 (1), 471-481.
21.Gbadamosi, O. K., Fasakin, E. A., & Adebayo, O. T. (2016). Hepatoprotective and stress - reducing effects of dietary Moringa oleifera extract against Aeromonas hydrophila infections and transportation-induced stress in Nile tilapia, Oreochromis niloticus (Linnaeus 1757) fingerlings. International Journal of Environmental and Agriculture Research (IJOEAR). 2 (7), 121-128.
22.Gbadamosi, O. K., Fasakin E. A., & Adebayo, O. T. (2017). Effects of Dietary Moringa oleifera Extract Against Aeromonas hydrophila infection and transportation-induced stress in African Catfish Clarias gariepinus (Burchell, 1822) fingerlings. World Applied Sciences Journal. 35 (1), 88-95.
23.Elabd, H., Soror, E., El-Asely, A., Abd El-Gawad, E., & Abbass, A. (2019). Dietary supplementation of Moringa leaf meal for Nile tilapia Oreochromis niloticus: Effect on growth and stress indices. Egyptian Journal of Aquatic Research. 45 (3), 265-271.
24.Emam, M. A., Shourbela, R. M., El-Hawarry, W. N., Abo-Kora, S. Y., Abdel-Monem Gad, F., Abd El-latif, A. M., & Dawood, M. A. O. (2022). Effects of Moringa oleifera aqueous extract on the growth performance, blood characteristics, and histological features of gills and livers in Nile tilapia. Aquaculture and Fisheries. https:// doi. org/10.1016/j.aaf.2021.12.011.
25.Sanchooli, N., Paknejad, H., Sudagar, M., Hosseini Shekarabi, S. P., & Rahdari, A. (2023). Growth promoting and immunomodulatory effects of dietary Moringa oleifera leaf powder in rainbow trout (Oncorhynchus mykiss). Aquaculture Reports. 30, 101555. https:// doi.org/10.1016/ j.aqrep. 2023. 101555.
26.Soltanian, S., & Fereidouni, M.S. (2016). Effect of Henna (Lawsonia inermis) extract on the immunity and survival of common carp, Cyprinus carpio infected with Aeromonas hydrophila. International Aquatic Research. 8, 247-261.
27.Kiadaliri, M., Firouzbakhsh, F., & Deldar, H. (2020). Effects of feeding with red algae (Laurencia caspica) hydroalcoholic extract on antioxidant defense, immune responses, and immune gene expression of kidney in rainbow trout (Oncorhynchus mykiss) infected with Aeromonas hydrophila. Aquaculture International 526, 735361.
28.Heydari, M., Firouzbakhsh, F., & Paknejad, H. (2020). Effects of Mentha longifolia extract on some blood and immune parameters, and disease resistance against yersiniosis in rainbow trout. Aquaculture International. 515, 734586.
29.Blaxhall, P. C., & Daisley, K. W. (1973). Routine haematological methods for use with fish blood. Journal of Fish Biology. 5 (6), 771-781.
30.Rehulka, J. (2000). Influence ofastaxanthin on growthrate, condition, and some blood indices of rainbow trout (Oncorhynchus mykiss). Journal of Aquaculture. 190 (1-2), 27-47.
31.Satheeshkumar, P., Ananthan, G., Kumar, D. S., & Jagadeesan, L. (2012). Haematology and biochemical parameters of different feeding behaviour of teleost fishes from Vellar estuary, India. Comparative Clinical Pathology. 21 (6), 1187-1191.
32.Abd El-Gawad, E. A. A., Asely, A. M. E., Soror, E. I., Abbass, A. A., & Austin, B. (2020). Effect of dietary Moringa oleifera leaf on the immune response and control of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus) fry. Aquaculture International. 28 (1), 389-402.
33.Bbole, I., Mumba, C., Mupenda, N., & Shula Kefi, A. (2016). Analysis of growth performance and haematological parameters of Oreochromis niloticus fed on a varying diet of Moringa oleifera Lam. leaf meal as an additive protein source. International Journal of Fisheries and Aquaculture. 8 (11), 105-111.
34.Sherif, A. H., El-Gamal, A. M., & Tolan, A. E. (2014). Incorporation of Moringa oleifera leaf in Nile tilapia Oreochromis niloticus diet and its effect on growth performance and immune status. Journal of Veterinary Science. 1, 806-814.
35.Lim, C., Klesius, P. H., Li, M. H., & Robinson, E. H. (2000). Interaction between dietary levels of iron and vitamin C on growth, hematology, immune response and resistance of channel catfish (Ictalurus punctatus) to Edwardsiella ictaluri challenge. Journal of Aquaculture. 185, 313-327.
36.Ozovehe, B. N. (2013). Growth performance, hematological indices and some biochemical enzymes of juveniles Clarias gariepinus [Burchell 1822] fed varying levels of Moringa oleifera leaf meal diet. Journal of Aquaculture Research and Development. 14 (2), 166-172.
37.Guardiola, F. A., Mabrok, M., Machado, M., Azeredo, R., Afonso, A., Esteban, M. A., & Costasa, B. (2019). Mucosal and systemic immune responses in Senegalese sole (Solea senegalensis Kaup) bath challenged with Tenacibaculum maritimum: A timecourse study. Fish and Shellfish Immunology. 87, 744–754.
38.Racicot, J. G., Gaudet, M., & Leray, C. (1975). Blood and liver enzymes in rainbow trout (Salmo gairdneri Rich.) with emphasis on their diagnostic use: Study of CCl4 toxicity and a case of Aeromonas infection. Journal of Fish Biology. 7 (6), 825-835.
39.Vutukuru, S. S., Pauleena, J. S., Rao, J. V., & Anjaneyulu, Y. (2007). Architectural changes in the gillmorphology of the freshwater fish, Esomus danricus as potential biomarkers of copper toxicity using automated video tracking system. Environmental Bioindicators. 2 (1), 3-14.
40.Tietz, N. W. (1986). Textbook of Clinical Chemistry. W. B. Saunders, Philadelphia, PA, USA.
41.Mansour, A. T., Espinosa, C., Garcia-Beltran, J. M., Miao, L., Ceballos Francisco, D. C., Alsaqufi, A. S., & Esteban, M. A. (2020). Dietary supplementation of drumstick tree, Moringa oleifera, improves mucosal immune response in skin and gills of seabream, Sparus aurata, and attenuates the effect of hydrogen peroxide exposure. Fish Physioloiogy Biochemistry. 46, 981-996.
42.Fakurazi, S., Sharifudin, S. A., & Arulselvan, P. (2012). Moringa oleifera hydroethanolic extracts effectively alleviate acetaminophen induced hepatotoxicity in experimental rats through their antioxidant nature. Molecules. 17 (7), 8334-8350.
43.Uma, N., Fakurazi, S., & Hairuszah, I. 2010. Moringa oleifera enhances liver antioxidant status via elevation of
the antioxidant enzyme activity and counteracts paracetamol-induced hepatotoxicity. Malays Journal Nutrition. 16 (2), 293-307.
44.Christian, E. O., Samuel, C. M., Chudi, E. D., Anaelechi, J. O., Chinwemma, F. O., Daniel, L. A., John, K. N., & Obi, E. (2016). Amelioratory effect of methanolic leaf extract of Moringa oleifera on some liver and kidney function and oxidative stress markers in lead intoxicated rats. European Journal of Medicinal Plants. 12 (4), 1-12.
45.Cao, L., Du, J., Ding, W., Rui, J., Liu, Y., Xu, P., Teraoka, H., & Yin, G. (2016). Hepaprotective and antioxidant effects of dietary Angelica sinensis extract against carbon tetrachloride-induced hepatic injury in Jian Carp (Cyprinius carpio var. Jian). Aquaculture Research. 47 (6), 1852-1863.
46.Rapatsa, M. M., & Moyo, N. A. G. (2014). Effect of Moringa oleifera on the histology, haematology and growth of Oreochromis mossambicus in the aquadams fertilised with chicken manure in South Africa. African Journal of Aquatic Science. 39, 295-303.
47.Nascimento, J. A., Magnani, M., Sousa, J. M. B., Araújo, K. L. G. V., Epaminondas, P. S., Souza, A. S., Souza, A. L., Silva, M. C. D., & Souza, A. G. (2016). Assessment of the antioxidant effects of Moringa oleifera Lam. Extracts in fish oil during storage. Journal of Food Processing and Preservation. 40 (1), 29-36.
48.Anwar, F., Latif, S., Ashraf, M., & Gilani, A. H. (2007). Moringa oleifera: a food plant with multiple medicinal uses. Phytother Research. 21 (1), 17-25.
49.Mohammadi, G., Rashidian, G., Hoseinifar, S. H., Naserabad, S. S., Van Doan, H. (2020). Ginger (Zingiber officinale) extract affects growth performance, body composition, haematology, serum and mucosal immune parameters in common carp (Cyprinus carpio). Fish and Shellfish Immunology. 99, 267-273.
50.Shourbela, R. M., El-Hawarry, W. N., Abd El-Latif, A. M., & Abo-Kora, S. Y. (2020). Potentiality of Moringa oleifera aqueous extract as a growth modulator and antistress in acute hypoxic Nile tilapia Oreochromis niloticus. Iranian Journal of Fisheries Science. 19 (1), 67-84.
51.Khalil, F., & Korni, F. M. M. (2017). Evaluation of Moringa oleifera leaves and their aqueous extract in improving growth, immunity and mitigating effect of stress on common carp (Cyprinus carpio) fingerlings. Turkish Journal of Aquatic Science. 32, 170-177.
52.Abdulkarim, S. M., Long, K., Lai, O. M., Muhammad, S. K. S., & Ghazali, H. M. (2005). Some physico-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chemistry. 93 (2), 253-263.
53.Sreelatha, S., & Padma, P. R. (2009). Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant Foods for Human Nutrition. 64 (4), 303-311.
54.Chumark, P., Khunawat, P., Sanvarinda, Y., Phornchirasilp, S., Morales, N. P., Phivthong-ngam, L., Ratanachamnong, P., Srisawat, S., & Pongrapeeporn, K. S. (2008). The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. Journal of Ethnopharmacology. 116 (3), 439-46.
55.Hajibeglou, A., & Sudagar M. (2010). Effects of using the valeriana officinalis extract during transportation of swordtail, Xiphophorus helleri. Journal of Animal and Veterinary Advances. 9 (18), 2377-2381.
مجله بهره برداری و پرورش آبزیان
دوره 12، شماره 3
مهر 1402
صفحه 29-47

فایل ها

هم رسانی

ارجاع به این مقاله

آمار