ارزیابی فعالیت آنتی‌اکسیدانی گیاه آبزی سنبل‌آبی (Eichhornia crassipes) تالاب انزلی با استفاده از روش استخراج غوطه‌وری

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه گیلان، صومعه‌سرا، ایران

2 نویسنده مسئول، گروه شیلات، دانشکده منابع طبیعی، دانشگاه گیلان، صومعه‌سرا، ایران و گروه علوم دریایی، پژوهشکده حوضه آبی دریای خزر، دانشگاه گیلان، رشت، ایران.

چکیده

سنبل‌آبی Eichhornia crassipes یکی از گیاهان آبزی مهاجم و غیر بومی تالاب انزلی است. در مطالعه حاضر تاثیر حلال‌های آبی، آبی- اتانولی (50:50) و اتانولی بر میزان خواص آنتی‌اکسیدانی عصاره‌های به دست آمده از قسمت‌های مختلف (برگ، ساقه و ریشه) سنبل آبی با روش استخراج غوطه‌وری (حلالی) بررسی شد. محتوای فنول کل، فعالیت خنثی‌کنندگی رادیکال آزاد، فعالیت آنتی‌اکسیدانی کل و قدرت کاهندگی آهن مورد سنجش قرار گرفت. نتایج نشان داد که بیش‌ترین مقدار فنول کل و بالاترین فعالیت خنثی‌کنندگی رادیکال‌ آزاد مربوط به عصاره‌ی آبی برگ بوده که دارای اختلاف معنی‌دار با سایر تیمارها بود (05/0P<). بالاترین فعالیت آنتی‌اکسیدانی کل مربوط به عصاره آبی- اتانولی برگ بوده که اختلاف معنی‌داری با سایر تیمارها داشت (05/0P<) و بالاترین قدرت کاهندگی آهن مربوط به عصاره‌ی آبی برگ بود (05/0P<). عصاره‌ی به دست آمده از برگ نسبت به ساقه و ریشه خاصیت آنتی‌اکسیدانی بالاتری را داشت. با توجه به نتایج، گیاه سنبل‌آبی می‌تواند به عنوان یک منبع آنتی‌اکسیدانی طبیعی ارزان قیمت و مناسب مورد استفاده قرار گیرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of antioxidant activity of water hyacinth (Eichhornia crassipes) from anzali wetland using immersion extraction method

نویسندگان [English]

  • Alireza Rabiepour 1
  • Aria Babakhani Lashkan 2
  • Eshagh Zakipour Rahimabadi 1
1 Dept. of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran
2 Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran
چکیده [English]

Eichhornia crassipes is one of the invasive and non-native aquatic plants of the Anzali wetland. In the present study, the effect of aqueous, aqueous-ethanolic (50:50), and ethanolic solvents was investigated on the amount of antioxidant properties of extracts obtained from different parts (leaf, stem, and root) of water hyacinth with Immersion (solvent) extraction method. Total phenol content, free radical scavenging activity, total antioxidant activity, and iron-reducing power were measured. The results showed that the highest amount of total phenol and the highest free radical neutralizing activity was related to the aqueous extract of the leaf, which had a significant difference from other treatments (P<0.05). The highest total antioxidant activity was related to the aqueous-ethanolic extract of the leaf, which had a significant difference from other treatments (P<0.05), and the highest iron reducing power was related to the aqueous extract of the leaf (P<0.05). The extract obtained from the leaf had higher antioxidant properties than the stem and root. According to the results, aquatic water hyacinth can be used as a cheap and suitable natural antioxidant source.

کلیدواژه‌ها [English]

  • Eichhornia crassipes
  • Anzali wetland
  • Aquatic plant
  • Antioxidant
  • Water hyacinth
1.Riley, P.A. 1994. Free radicals in biology: oxidative stress and effects of ionizing radiation, International Journal of Radiation Biology, 65: 27-33.
2.Halliwell, B. 1996. Antioxidants in human health and disease, Annual review of nutrition, 16: 1. 33-50.
3.Sindhi, V., Gupta V., Sharma, K., Bhatnagar, S., Kumari, R., and Dhaka, N. 2013. Potential applications of antioxidant- A review. Journal of Pharmacy Research. 7: 828-835.
4.Collins, A.R. 2005. Antioxidant intervention as a route to cancer prevention. European Journal of Cancer. 41: 13. 1923-1930.
5.Halliwell, B. 2007. Biochemistry of oxidative stress. Biochemical society transactions. 35: 5. 1147-1150.
6.Linderschmidt, R., Trylka, A., Goad, M., and Witschi, H. 1986. The effects of dietary butylated hydroxytoluene on liver and colon tumor development in mice. Toxicology. 38: 151-160.
7.Olukemi, O.A., Olukemi, I.O., Oluwatoyin, S.M., Austin, A.O., Mansurat, L.B., and Olufunmilola, T.I. 2005. Antioxidant activity of Nigerian dietary spices. Electronic Journal of Environmental, Agricultural and Food Chemistry. 4: 6. 1086-1093.
8.Saviz, A., and Pourmoradi, S. 2013. Importance and application of antioxidants. Third National Conference on Food Safety, Islamic Azad University, Savadkuh Branch, Savad Kooh, Iran. (In Persian)
9.Ebrahimzadeh, M.A., Nabavi, S.M., Nabavi, S.F., and Eslami, B. 2010. Antioxidant activity of the bulb and aerial parts of Ornithogalum sintenisii L (Liliaceae) at flowering stage. Tropical Journal of Pharmaceutical Research. 9: 2. 141-148.
10.Mishra, N.B., and Ranjan, R. 2008. Growth of hairy-root culture in variuth bioreactors for the production of secondary metabolites. Biotechnology and Applied Biochemistry. 49: 1-10.
11.Kumar, G.D., and Rajakumar, R. 2016. Gas Chromatography-Mass Spectrometry Analysis of Bioactive Components from the Ethanol Extract of Avicennia Marina Leaves. Innovare Journal of Science. 4: 4. 9-12.
12.Keshari, R.B., Kumar R.S., and Madhab K.D. 2011. Mental health challenges and possible solutions with special reference to anxiety. International Research Journal of Pharmacy. 2: 9. 37-42.
13.Vesali Naseh, M.R., Karbassi, A.R., Ghazaban, F., and Baghvand, A. 2012. Evaluation of heavy metal pollution in Anzali wetland. Guilan, Iran, Iranian Journal of Toxicology. 15: 5. 565-576.
14.Khosheghbal, M.Z., Charkhabi, A.H., Sharifi, F., and Ghazban, F. 2013. An investigation of sediment pollution in the Anzali wetland. Polish Journal of Environmental Studies, 22: 1. 283-288.
15.Zamani Hargalani, F., Karbassi, A., and Monavari, S.M., and Abroomand Azar, P. 2014. A novel pollution index based on the bioavailability of elements: a study on Anzali wetland bed sediments. Environmental Monitoring and Assessment. 186: 4. 2329-2348.
16.Mirzajani, A., Naderi, S., and Parvaneh Moghadam, D. 2019. Distribution survey and some biological aspects of Water Hyacinth in Anzali Wetland, Guilan province. Iranian Journal of Plant Biology. 11: 4. 51-62. (In Persian)
17.Rufchaie, R., Mirvaghefei, A., and Abdollahi, V. 2016. Evaluation of native phytobiotic effects on immune response of fish and shellfish. Journal of Utilization and Cultivation of Aquatics. 5: 2. 1-16. (In Persian)
18.Parsons, W.T., Parsons, W.T., and Cuthbertson, E. 2001. Noxious weeds of Australia. CSIRO publishing.
19.Gunnarsson, C.C., Petersen, and C.M. 2007. Water hyacinths as a resource in agriculture and energy production:
a literature review. Waste Manage. 27: 117-129.
20.Laranjeira, C.M., and Nadais, G. 2008. Eichhornia crassipes control in the largest Por-tuguese natural freshwater lagoon. EPPO Bulletin. 38: 3. 487-495.
21.Shanab, S.M.M., and Shalaby, E.A. 2012. Biological activities and anticorrosion efficiency of water hyacinth (Eichhornia crassipes). Journal of Medicinal Plants Research. 23: 6. 3950-3962.
22.Sindhu, R., Binod, P., Pandey, A., Madhavan, A., Alphonsa, J.A., Vivek, N., Gnansounou, E., Castro, E., and Faraco, V. 2017. Water hyacinth a potential source for value addition: An overview. Bioresource Technology. 230: 152-162.
23.Gaurav, G.K., Mehmood, T., Cheng, L., Klemes, J.J., and Shrivastava, D.K. 2020. Water hyacinth as a biomass: A review. Journal of Cleaner Production. 277: 122214.
24.Haggag, M.W., Abou El Ella, S.M., and Abouziena, H.F. 2017. Phytochemical Analysis, antifungal, antimicrobial activities and application of Eichhornia crassipes against some plant pathogens. Planta Daninha, 35p.
25.Tulika, T., Puneet, P., and Mala, A. 2017. Qualitative phytochemical analysis and antioxidant activity of methonolic extract of Eichhornia crassipes (Mart) Solms and Pistia stratiotes L. International Journal of Pharmacognosy and Phytochemical Research. 9: 632-636.
26.Agunbiade, F.O., Olu-Owolabi, B.I., and Adebowale, K.O. 2009. Phytoremediation potential of Eichornia crassipes in metal-contaminated coastal water. Bioresource Technology. 100: 19. 4521-4526.
27.Jafari, N. 2010. Ecological and socio-economic utilization of water hyacinth (Eichhornia crassipes). Journal of Applied Sciences and Environmental Management. 14: 2. 43-49.
28.Pandey, D.K. 2015. Allelochemicals from Parthenium for water hyacinth control. Indian Journal of Weed Science. 47: 3. 321-328.
29.Tham, H.T. 2015. Utilization of water hyacinth as animal feed. Nova Journal of Engineering and Applied Sciences.
4: 1. 1-6.
30.Li, X., Zhou, Y., Yang, Y., Yang, S., Sun, X., and Yang, Y. 2015. Physiological and proteomics analyses reveal the mechanism of Eichhornia crassipes tolerance to high-concentration cadmium stress compared with Pistia stratiotes. PLoS One. 10: 4. 1-22.
31.Patel, S. 2012. Threats, management and envisaged utilizations of aquaticweed Eichhornia crassipes: an overview. Reviews in Environmental Science and Bio/Technology. 11: 3. 249-259.
32.Komy, Z.R., Abdelraheem, W.H., and Ismail, N.M. 2013. Biosorption of Cu2+ by Eichhornia crassipes: Physicochemical characterization, biosorption modeling andmechanism. Journal of King Saud University-Science. 25: 1. 47-56.
33.Gunja, Vipin, G., Priyanka, J., Kant, S.C., Dixit, A.K., and Jain, R.K. 2016. Production of bioethanol from water hyacinth by isolated thermotolerant bacteria. International Journal of Current Science and Technology. 4: 219-223.
34.Cardoso, S.F., Lopes, L.M.X., and Nascimento, I.R. 2014. Eichhornia crassipes: an advantageous source of shikimic acid. Revista Brasileira de Farmacognosia. 24: 439-442.
35.Lenora, L.M., Babu, D.S., Kumar, J.S., and Kumar, N.S. 2016. Eicchornia crassipes (Mart.) Solems – an alternative renewable source for shikimic acid, a precursor for Tamiflu, a swineflu drug. Journal Pharmacognosy Phytochemistry. 5: 1. 178-181.
36.Ramirez, N.F., Hernandez, Y.S., Leon, J.C., Garcia, S.R.V., Lvova, L.D., and Gonzalez, L.G. 2015. Composites from water hyacinth (Eicchornia crassipes) and polyester resin. Fibres and Polymers. 16: 1. 196-200.
37.Onchonga, N.A., Oima, D., and Oginda, M. 2013. Utilization of water hyacinth as an alternative substrate for mushroom farming: a study of vihiga mushroom project in western Kenya. International Journal of Educational Research. 1: 1-10.
38.Rufchaie, R., Mirvaghefi, A., Hoseinifar, S.H., and Valipour, A. 2019. Anti-microbial Activity of Echhornia Crasippes Aquatic and Hydromethanolic Leaves Extract. Journal of Fisheries. 71: 1. 31-41. (In Persian)
39.Silva, R.P., de Melo, M.M., Silvestre, A.J., and Silva, C.M. 2015. Polar and lipophilic extracts characterization of roots, stalks, leaves and flowers of water hyacinth (Eichhornia crassipes), and insights for its future valorization. Industrial Crops and Products. 76: 1033-1038.
40.Khalid, S., Shaheen, S., Hussain, K., Shahid, N., and Sarwar, S. 2020. Pharmacological analysis of obnoxious water weed: Eichhornia crassipes (Mart) Solis. The Journal of Animal & Plant Sciences. 30: 6. 1465-1475.
41.Prieto, P., Pineda, M., and Aguilar, M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 269: 2. 337-341.
42.Taga, M.S., Miller, E.E., and Pratt, D.E. 1984. Chia seeds as a source of natural lipid antioxidants. Journal of the American Oil Chemists’ Society. 61: 5. 928-931.
43.Brand-Williams, W., Cuvelier, M.E., and Berset, C.L. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology. 28: 1. 25-30.
44.Chew, Y.L., Lim, Y.Y., Omar, M., and Khoo, K.S. 2008. Antioxidant activity of three edible seaweeds from two areas in South East Asia. LWT-Food Science and Technology. 41: 6. 1067-72.
45.Mendiola, J.A., Rodriguez- Meizoso, I., Senorans, F.J., Reglero, G., Cifuentes, A., and Ibannez, E. 2008. Antioxidants in plant foods and microalagae extracted using compressed fluids. Electronic Journal of Environment. Agricultural and Food Chemistry. 7: 10. 3301-3309.
46.Zhou, Y., Zheng, J., Gan, R.Y., Zhou, T., Xu, D.P., and Bin Li, H. 2017. Optimization of ultrasound-assisted extraction of antioxidants from the mung bean coat. Molecules. 22: 4. 1-13.
47.Yang, Q.Q., Gan, R.Y., Ge, Y.Y., Zhang, D., and Corke, H. 2019. Ultrasonic treatment increases extraction rate of common bean (Phaseolus vulgaris L.) antioxidants. Antioxidants. 8: 4. 83.
48.Sopee, M.S.M., Azlan, A., and Khoo, H.E. 2019. Comparison of antioxidants content and activity of Nephelium mutabile rind extracted using ethanol and water. Journal of Food Measurement and Characterization, 13: 3. 1958-1963.
49.Wei, Y., Fang, Z., Zheng, L., and Tsangb, E.P. 2017. Biosynthesized iron nanoparticles in aqueous extracts of Eichhornia crassipes and its mechanism in the hexavalent chromium removal. Applied Surface Science. 399: 322-329.
50.Mamelona, J., Pelletier, E., Girard-Lalancette, K., Legault, J., Karboune, S., and Kermasha, S. 2007. Quantification of phenolic contents and antioxidant capacity of Atlantic sea cucumber Cucumaria frondosa. Food Chemistry. 104: 1040-1047.
51.Zhang, G., He, L., and Hu, M. 2011. Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innovative Food Science and Emerging Technologies. 12: 1. 18-25.
52.Borzoie, H., and Tabarsa, M. 2017. The use of secondary metabolites of Eichhornia crassipes in neutralizing free radicals, The Second National Conference on Science and Technology of Agricultural Sciences, Natural Resources and Environment of Iran, Tehran, Iran. (In Persian)
53.Naghdi, S., Babakhani Lashkan, A., and Rashidiyan, G. 2021. Ultrasound-assisted extraction of phenolic compounds from Azolla filiculoides using Taguchi method: Antioxidant and antibacterial capabilities. Iranian Journal of Fisheries Sciences, 20: 5. 1354-1370.
54.López-Mejía, O.A., López-Malo, A., and Palou, E. 2014. Antioxidant capacity of extracts from amaranth (Amaranthus hypochondriacus L.) seeds or leaves. Industrial Crops and Products. 53: 55-59.
55.Wong, K.W.A. 2017. Antioxidant properties of wild Nelumbo nucifera (lotus plant) grown in Ipoh. Universiti Tunku Abdul Rahman.
56.Gao, H., Zhang, L., Zhu, A., Liu, X., Wang, T., Wan, M., Yang, X., Zhang, Y., and Zhang, Y. 2020. Metabolic profiling of nuciferine in vivo and in vitro. Journal of Agricultural and Food Chemistry. 68: 48. 14135-14147.
57.Marzouk, Z., Marzouk, B., Mahjoub, M.A.A, Haloui, E., Mighri, Z., Aouni, M., and Fenina, N. 2010. Screening of the antioxidant and the free radical scavenging potential of Tunisian Citrullus colocynthis Schrad. from Mednine. Journal of Food Agricuture and Environment, 8: 2. 261-265.
58.Garmsiri, E., Rezaei, M., Shaviklo, A., and Babakhani Lashkan, A. 2013. Antioxidant Activity and Total Phenolic Contents of Persian Gulf Red Algae (Hypnea hamulosa). Journal of Utilization and Cultivation of Aquatics. 2: 3. 37-48. (In Persian)
59.Moure, A., Franco, D., Sineiro, J., Domĭngguez, H., Nūňez, M.J., and Lema, J.M. 2001. Antioxidant activity of extracts from Gevuina avellana and Rosa rubiginosa defatted seeds. Food Research International. 34: 2.103-109.
60.Baradaran, M., Ashrafpour, M., Rezaei, H., Sefidgar, A.A., and Sharifi, H. 2014. Antioxidant activity of different extracts of Artemisia Annua growing in an area of Babol city, Sabzevar University of Medical Sciences, 21: 4. 529-539. (In Persian)
61.Karadag, A., Ozcelik, B., and Saner, S. 2009. Review of methods to determine antioxidant capacities. Food analytical methods. 2: 1. 41-60.
62.Soto-Maldonado, C., Fernández-Araya, B., Saavedra-Sánchez, V., Santis-Bernal, J., Alcaíno-Fuentes, L., Arancibia-Díaz, A., and Zúñiga-Hansen, M.E. 2022. Antioxidant and antimicrobial capacity of Maytenus boaria leaves, recovery by infusion and solvent extraction. Electronic Journal of Biotechnology. 56: 47-53.
63.Jayanthi, P., and Lalitha, P. 2011. Determination of the in vitro reducing power of the aqueous extract of Eichhornia crassipes (Mart.) Solms. Journal of Pharmacy Research. 4: 4003-4005.
64.Alam, M., Biozid, M.S., Faruk, M., Abeden, M.J., Ferdous, K.U., Nitul, I.A., and Islam, M.R. 2020. Anti-oxidant activity of methanolic extract of aquatic flowering plant Nymphaea capensis leaf.
65.Hodhodi, A., Babakhani, A., and Rostamzad, H. 2020. Extraction of brown alga Sargassum angustifolium and evaluation of fluorotanine compounds and its antioxidant properties, Journal of Fisheries Science and Technology. 9: 3. 156-169. (In Persian)
66.Marmouzi, I., Kharbach, M., El Jemli, M., Bouyahya, A., Cherrah, Y., Bouklouze, A., Vander Heyden, Y., and Faouzi, M.E.A. 2019. Antidiabetic, dermatoprotective, antioxidant and chemical functionalities in Zizyphus lotus leaves and fruits. Industrial Crops and Products. 132: 134-139.