اثر مکمل غذایی بوتیرات سدیم بر برخی شاخص‌های خونی، ایمنی و پاسخ به تنش تراکم فیل ماهی جوان (Huso huso)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 نویسنده مسئول، دانشجوی دکتری تکثیر و پرورش آبزیان، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 استاد گروه شیلات، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

3 استادیار پژوهشی، پژوهشکده اکولوژی دریای خزر، ساری، ایران

چکیده

این مطالعه با هدف بررسی پاسخ فیزیولوژیکی فیل ماهیان جوان به سطوح مختلف بوتیرات سدیم در جیرة غذایی انجام پذیرفت. بدین منظور تعداد 360 قطعه فیل ماهی جوان با میانگین وزنی 56/0 ± 15 گرم با 6 جیرة آزمایشی حاوی 0، 1/0، 2/0، 3/0، 4/0 و 5/0 درصد بوتیرات سدیم به مدت 8 هفته تغذیه شدند. فیل‌ماهیان مورد مطالعه به‌طور تصادفی به 18 حوضچه‌ آزمایشی به حجم 2/3 مترمکعب (3200 لیتر) و حجم آبگیری 1 مترمکعب (1000 لیتر) انتقال داده ‌شدند (هر حوضچه 20 قطعه ماهی) و روزانه 6 بار با جیره‌های آزمایشی در حد سیری تغذیه شدند. تعداد گلبول‌های قرمز خون (RBC) و شاخص‌‌های MCV، MCH و MCHC در تیمار 3/0 درصد از سایر تیمارها به‌طور معنی‌داری بیشتر بود (05/0 p<). تعداد گلبول‌های سفید خون (WBC) و مقدار هموگلوبین و هماتوکریت در ماهیان تغذیه شده با 3/0 درصد بوتیرات سدیم، بیشتر از سایر تیمارها بود، اگرچه تفاوت معنی‌داری با تیمار 4/0 و گروه شاهد نداشت (05/0 <p). فعالیت لیزوزیم و میزان IgM در تیمار 3/0 درصد به‌طور معنی‌داری از سایر تیمارها بالاتر بود (05/0 >p). میزان کورتیزول و گلوکز در فیل‌ماهیان تغذیه‌شده با جیرة حاوی 3/0 درصد بوتیرات سدیم پس از تنش تراکم حاد نسبت به گروه‌های دیگر به میزان کمتری افزایش یافت و اختلاف معنی‌دار بین تیمارها مشاهده شد (05/0>p). در مجموع، نتایج این مطالعه نشان می‌دهد که گنجاندن 3/0 درصد بوتیرات سدیم در جیرة غذایی فیل ماهیان جوان، می‌تواند به سلامت ماهیان، بهبود پاسخ ایمنی ذاتی و مقاومت در برابر تنش‌های تراکم کمک کند,

کلیدواژه‌ها

عنوان مقاله [English]

Effects of sodium butyrate dietary supplement on some haematological parameters, immunity and intestinal microbiota and response to stocking density stress of young beluga (Huso huso)

نویسندگان [English]

  • Hossein Panahi Sahebi 1
  • Hossein Oraji 2
  • Farid Firouzbakhsh 2
  • Maryam Ghiasi 3
1 Corresponding Author, Ph.D. Student of Aquatic Reproduction and Breeding, Faculty of Animals Sciences and Fisheries, Sari Agricultural Sciences and Natural Resource University, Sari, Iran
2 Professor, Dept. of Fisheries, Faculty of Animals Sciences and Fisheries, Sari Agricultural Sciences and Natural Resource University, Sari, Iran
3 . Research Assistant Prof., Caspian Sea Ecology Research Center, Sari, Iran
چکیده [English]

The aim of this study was to investigate the physiological response of young beluga to different levels of sodium butyrate in the diet. For this purpose, 360 young beluga (average weight of 15 ± 0.56 g) with 6 experimental diets containing 0, 0.1, 0.2, 0.3, 0.4 and 0.5% sodium butyrate were fed for 8 weeks. The number of red blood cells (RBC) and MCV, MCH and MCHC indices were significantly higher in the 0.3% treatment than in the other treatments (p <0.05). The number of white blood cells (WBC) and the amount of hemoglobin and hematocrit in fish fed with 0.3% sodium butyrate in the feed were higher than other treatments, although there was no significant difference with the treatment of 0.4% and the control group (p >0.05). Lysozyme activity and IgM level in 0.3% treatment was significantly higher than other treatments (p <0.05). Cortisol and glucose levels in fish fed diets containing 0.3% sodium butyrate increased slightly less than other groups after acute density stress and a significant difference was observed between treatments (p <0.05). Overall, this study indicates that including 0.3% sodium butyrate in the diet of young beluga can improve fish health, innate immune response and resistance to density stress.

کلیدواژه‌ها [English]

  • Beluga
  • Sodium butyrate
  • Haematological parameters
  • Immune response
  • Density stress

مراجع

1.Dawood, MAO., Koshio, S., Abdel-Daim, MM., and Van Doan, H. 2019. Probiotic application for sustainable aquaculture. Review Aquaculture. 11: 3. 907-924.
2.Alderman, D., and Hastings, T. 1998. Antibiotic use in aquaculture: development of antibiotic resistance–potential for consumer health risks. International journal of food science & technology. 33: 2. 139-155.
3.Holmström, K., Gräslund, S., Wahlström, A., Poungshompoo, S., Bengtsson, B.E., and Kautsky, N. 2003. Antibiotic use in shrimp farming and implications for environmental impacts and human health. International journal of food science & technology. 38: 255-266.
4.Cabello, F.C. 2006. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environmental microbiology. 8: 1137-1144.
5.Romero, J., Feijoó, C.G., and Navarrete, P. 2012. Antibiotics in aquaculture–use, abuse and alternatives. Health and environment in aquaculture. 159p.
6.Hoseinifar, S.H., Sun, Y.Z., and Caipang, C.M. 2017. Short‐chain fatty acids as feed supplements for sustainable aquaculture: An updated view. Aquaculture Research. 48: 1380-1391.
7.Wang, W., Sun, J., Liu, C., and Xue, Z. 2017. Application of immunostimulants in aquaculture: current knowledge
and future perspectives. Aquaculture Research. 48: 1-23.
8.Dawood, M.A., Koshio, S., Ishikawa, M., Yokoyama, S., El Basuini, M.F., Hossain, M.S., Nhu, T.H., Dossou, S., and
Moss, A.S. 2016. Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major. Fish & Shellfish Immunology.49: 275-285.
9.Ng, W.K., and Koh, C.B. 2017. The utilization and mode of action of organic acids in the feeds of cultured aquatic animals. Reviews in Aquaculture. 9: 342-368.
10.Abdel-Mohsen, H.H., Wassef, E.A., El-Bermawy, N.M., Abdel-Meguid, N.E., Saleh, N.E., Barakat, K.M., and Shaltout, O.E. 2018. Advantageous effects of dietary butyrate on growth, immunity response, intestinal microbiota and histomorphology of European Seabass (Dicentrarchus labrax) fry. Egyptian Journal of Aquatic Biology and Fisheries. 22: 4. 93-110.
11.Robles, R., Lozano, A., Sevilla, A., Márquez, L., Nuez-Ortín, W., and Moyano, F. 2013. Effect of partially protected butyrate used as feed additive on growth and intestinal metabolism in sea bream (Sparus aurata). Fish Physiology and Biochemistry. 39: 1567-1580.
12.Owen, M., Waines, P., Bradley, G., and Davies, S. 2006. The effect of dietary supplementation of sodium butyrate on the growth and microflora of Clarias gariepinus (Burchell 1822). In: (Eds.), Proceeding of proceedings of the XII international symposium fish nutrition and feeding. 250p.
13.Sun, L., Liu, Z., Hao, G., Zhou, L., Lu, S., Zhang, J., and Xiao, T. 2013. Effects of sodium butyrate on growth and intestinal cell proliferation of Carassius auratus. Journal Fish Science China.20: 893-901.
14.Aalamifar, H., Soltanian, S., Vazirzadeh, A., Akhlaghi, M., Morshedi, V., Gholamhosseini, A., and Torfi Mozanzadeh, M. 2020. Dietary butyric acid improved growth, digestive enzyme activities and humoral immune parameters in Barramundi (Lates calcarifer). Aquaculture Nutrition. 26: 1. 156-164.
15.Liu, W., Yang, Y., Zhang, J., Gatlin, D.M., Ringø, E., and Zhou, Z. 2014. Effects of dietary microencapsulated sodium butyrate on growth, intestinal mucosal morphology, immune response and adhesive bacteria in juvenile common carp (Cyprinus carpio) pre-fed with or without oxidised oil. British Journal of Nutrition. 112: 15-29.
16.Ahmed, H., and Sadek, K. 2015. Impact of dietary supplementation of sodium butyrate and/or protexin on the growth performance, some blood parameters, and immune response of Oreochromis niloticus. International Journal of Agriculture Innovations and Research. 3: 4. 985-991.
17.Dawood, M.A., Eweedah, N.M., Elbialy, Z.I., and Abdelhamid, A.I. 2020. Dietary sodium butyrate ameliorated the blood stress biomarkers, heat shock proteins, and immune response of Nile tilapia (Oreochromis niloticus) exposed to
heat stress. Journal of Thermal Biology. 88: 102500.
18.Liu, M., Guo, W., Wu, F., Qu, Q.,Tan, Q., and Gong, W. 2017. Dietary supplementation of sodium butyratemay benefit growth performance and intestinal function in juvenile grass carp (Ctenopharyngodon idellus). Aquaculture Research. 48: 4102-4111.
19.Ali, TE-S., El-Sayed, AM., Eissa, MA. R., and Hanafi, HM. 2018. Effect of dietary supplementation of sodium butyrate on growth performance and feed utilization of Nile tilapia (Oreochromis niloticus) fries. Indian Journal of Geo-Marine Sciences.47: 2071-2076.
20.Najdegerami, E.H., Tran, T.N., Defoirdt, T., Marzorati, M., Sorgeloos, P., Boon, N., and Bossier, P. 2012. Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community. FEMS Microbiology Ecology. 79: 25-33.
21.Adjoumani, J.J.Y., Wang, K., Zhou, M., Liu, W., and Zhang, D. 2017. Effect of dietary betaine on growth performance, antioxidant capacity and lipid metabolism in blunt snout bream fed a high-fat diet. Fish physiology and biochemistry. 43: 6. 1733-1745.
22.Stoskpofe, M.A. 1993. Fish Medicine. Sounders Company. USA. 882p.
23.Klontz, G. 1994. Fish hematology. Techniques in fish immunology.3: 121-131.
24.Ellis, A. 1990. Lysozyme Assays: In Stolen, J.S., Fletcher, T.C., Anderson, D.P., Roberson, B.S., Van Muiswinkel, W.B., editors. Techniques in: Fish Immunology. Fair Haven. NJ: SOS Publications. pp. 101-103.
25.Reyes-Becerril, M., Tovar-Ramírez, D., Ascencio-Valle, F., Civera-Cerecedo, R., Gracia-López, V., and Barbosa-Solomieu, V. 2008. Effects of dietary live yeast Debaryomyces hansenii on the immune and antioxidant system in juvenile leopard grouper Mycteroperca rosacea exposed to stress. Aquaculture. 280: 39-44.
26.Falahatkar, B., Poursaeid, S., Shakoorian, M., and Barton, B. 2009. Responses to handling and confinement stressors in juvenile great sturgeon Huso huso. Journal of Fish Biology, 75: 4. 784-796.
27.Barry, T.P., Lapp, A.F., Kayes, T.B., and Malison, J.A. 1993. Validation of a microtitre plate ELISA for measuring cortisol in fish and comparison of stress responses of rainbow trout (Oncorhynchus mykiss) and lake trout (Salvelinus namaycush). Aquaculture. 117: 351-363.
28.Buscaino, G., Filiciotto, F., Buffa, G., Bellante, A., Di Stefano, V., Assenza, A., Fazio, F., Caola, G., and Mazzola, S. 2010. Impact of an acoustic stimulus on the motility and blood parameters of European sea bass (Dicentrarchus labrax L.) and gilthead sea bream (Sparus aurata L.). Marine environmental research. 69: 3. 136-142.
29.Fazio, F., Marafioti, S., Arfuso, F., Piccione, G., and Faggio, C. 2013. Comparative study of the biochemical and haematological parameters of four wild Tyrrhenian fish species. Veterinární medicína, 58: 11. 576-581.
30.Magnadottir, B. 2006. Innate immunity of fish (overview). Fish and Shellfish Immunology. 20: 137-151.
31.Reyshari, A., Mohammadiazarm, H., Mohammadian, T., and Torfi Mozanzadeh, M. 2019. Effects of sodium diformate on growth performance, gut microflora, digestive enzymes and innate immunological parameters of Asian sea bass (Lates calcarifer) juveniles. Aquaculture Nutrition. 25: 1135-1144.
32.Chow, E.P.Y., Liong, K.H., and Schoeters, E. 2017. Dietary encapsulated butyric acid (Butipearl™) and microemulsified carotenoids (Quantum GLO™ Y) on the growth, immune parameters and their synergistic effect on pigmentation of hybrid
Catfish (Clarias macrocephalus× Clarias gariepinus). Fish Aquaculture Journal. 8: 2.
33.Tian, L., Zhou, X.Q., Jiang, W.D., Liu, Y., Wu, P., Jiang, J., Kuang, S.Y., Tang, L., Tang, W.N., and Zhang, Y.A. 2017. Sodium butyrate improved intestinal immune function associated with NF-κB and p38MAPK signalling pathways in young grass carp (Ctenopharyngodon idella). Fish & shellfish immunology. 66: 548-563.
34.Abd El-Naby, A.S., Khattaby, A.E.R.A., Samir, F., Awad, S.M., and Abdel-Tawwab, M. 2019. Stimulatory effect of dietary butyrate on growth, immune response, and resistance of Nile tilapia, Oreochromis niloticus against Aeromonas hydrophila infection. Animal Feed Science and Technology. 254: 114-212.
35.Jesus, G.F., Pereira, S.A., Owatari, M.S., Syracuse, N., Silva, B.C., Silva, A., Pierri, B.S., Lehmann, N.B., Figueiredo, H.C., and Fracalossi, D.M. 2019. Protected forms of sodium butyrate improve the growth and health of Nile tilapia fingerlings during sexual reversion. Aquaculture. 499: 119-127.
36.Mirghaed, A.T., Yarahmadi, P., Soltani, M., Paknejad, H., and Hoseini, S.M. 2019. Dietary sodium butyrate (Butirex® C4) supplementation modulates intestinal transcriptomic responses and augments disease resistance of rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology. 92: 621-628.
37.Rotllant, J., and Tort, L. 1997. Cortisol and glucose responses after acute stress by net handling in the sparid red porgy previously subjected to crowding stress. Journal of Fish Biology, 51: 1. 21-28.
38.Barton, B.A. 2002. Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and comparative biology, 42: 3. 517-525.
39.Barton, B.A., Rahn, A.B., Feist, G., Bollig, H., and Schreck, C.B. 1998. Physiological stress responses of the freshwater chondrostean paddlefish (Polyodon spathula) to acute physical disturbances. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 120: 2. 355-363.
40.Bayunova, L., Barannikova, I., and Semenkova, T. 2002. Sturgeon stress reactions in aquaculture. Journal of Applied Ichthyology, 18: 4‐6. 397-404.
41.Hamlin, H.J., Edwards, T.M., Moore, B.C., Main, K.L., and Guillette Jr, L.J. 2007. Stress and its relation to endocrine function in captive female Siberian sturgeon (Acipenser baeri). Environmental sciences: an international journal of environmental physiology and toxicology, 14: 3. 129-139.
42.Webb, M.A., Allert, J.A., Kappenman, K.M., Marcos, J., Feist, G.W., Schreck, C.B., and Shackleton, C.H. 2007. Identification of plasma glucocorticoids in pallid sturgeon in response to stress. General and Comparative Endocrinology, 154: 1-3. 98-104.
مجله بهره برداری و پرورش آبزیان
دوره 11، شماره 4
دی 1401
صفحه 95-107

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