استخراج فیکوسیانین از جلبک اسپیرولینا پلاتنسیس و ارزیابی پایداری نانولیپوزوم‌های حامل رنگدانه در برابر شرایط محیطی

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری فرآوری محصولات شیلاتی، دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران.

2 نویسنده مسئول، دانشیار گروه فرآوری محصولات شیلاتی، دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران.

3 دانشیار گروه فرآوری محصولات شیلاتی، دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران.

4 دانش‌آموخته دکتری فرآوری محصولات شیلاتی، دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

10.22069/japu.2021.19704.1620

چکیده

تکنیک درون پوشانی در نانوحامل‌های لیپیدی می‌تواند روشی کارآمد برای برطرف نمودن محدودیت‌های موجود در استفاده از رنگدانه فیکوسیانین مستخرج از ریزجلبک اسپیرولینا به دلیل طعم و بوی خاص، حساسیت بالای این ترکیبات و ایجاد رنگ نامطلوب در محصولات باشد. لذا، در مطالعۀ حاضر رنگدانۀ فیکوسانین از جلبک اسپیرولینا پلاتنسیس استخراج و غلظت آن مورد بررسی قرار گرفت. نانولیپوزوم‌های حاوی رنگدانه با پوشش کیتوزان (1%) و فاقد پوشش تهیه شد و خصوصیات فیزیکوشیمیایی، مورفولوژی و پایداری آن نسبت به شرایط مختلف محیطی (رطوبت، دما و نور) ارزیابی گردید. میانگین اندازه نانولیپوزوم‌ها و شاخص پراکندگی به‌ترتیب از محدودۀ 21/322 تا 31/426 نانومتر و 27/0 تا 28/0 در نانوحامل‌ها متغیر بود. بالاترین مقادیر راندمان نانوپوشانی نانولیپوزوم حاوی فیکوسیانین (4/81 درصد) تحت‌ شرایط بهینه در نانولیپوزوم با پوشش کیتوزان به‌دست‌آمد. ارزیابی ثبات لیپوزوم ها در برابر نور، رطوبت نسبی و دماهای مختلف نگهداری طی زمان حاکی از افزایش پایداری فیکوسیانین محصور شده در حامل های لیپیدی بوده و کیتوزان به عنوان پوشش لیپوزوم، سبب افزایش ثبات و کنترل انتشار پایدار فیکوسیانین گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Extraction of phycocyanin from spirulina microalgae and evaluation of the stability of nanoliposomes incorporated with pigment against environmental conditions

نویسندگان [English]

  • Mahin Rigi 1
  • Seyed Mahdi Ojagh 2
  • Alireza Alishahi 3
  • Shirin Hasani 4
1 Ph.D. Student of Sea Food Processing, Faculty of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Corresponding Author, Associate Prof., Dept. of Sea Food Processing, Faculty of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
3 Associate Prof., Dept. of Sea Food Processing, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
4 Ph.D. Graduate of Sea Food Processing, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
چکیده [English]

The encapsulation technique in lipid nanocarriers can be an effective way to resolve the limitations in the utilization of phycocyanin pigment extracted from spirulina due to special taste and odor, high sensitivity of these compounds, and undesirable color in products. Therefore, in the present study, phycocyanin was extracted from Spirulina platensis and its concentration was investigated. Nanoliposomes with chitosan coating (0 and 1% w/v) containing pigment were prepared and its physicochemical properties, morphology, and stability to different environmental conditions (relative humidity, temperature, and light) were evaluated. The mean size of nanoliposomes and polydispersity index ranged from 322.21 to 426.31 nm and from 0.27 to 0.28 in nanocarriers, respectively. The highest values of encapsulation efficiency of nanoliposome containing phycocyanin (81.4%) were obtained under optimal conditions in nanoliposomes with chitosan coating. Evaluation of the stability of liposomes against light, relative humidity, and different temperatures over storage showed an increase in the stability of phycocyanin encapsulated in lipid carriers and chitosan as a liposome coating increased the stability and controlled release of phycocyanin.

کلیدواژه‌ها [English]

  • Phycocyanin
  • nanoliposomes
  • Morphology
  • Stability
  • relative humidity
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