شناسایی ماهی آزاد دریای خزر (Kessleri, 1877) Salmo caspius و قزل‌آلای رنگین‌کمان (Walbaum, 1792) Onchorhyncus mykiss فرار کرده از قفس‌های پرورشی با استفاده از توالی‌یابی ژن Cyt b در حوضه جنوبی دریای خزر

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری تولید و بهره‌برداری آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

2 نویسنده مسئول، استاد گروه تولید و بهره‌برداری آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

3 دانشیار گروه محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران.

4 دانشیار گروه منابع آبزی، بخش تحقیقات ساحلی، دانشگاه علوم کشاورزی اپسالا، سوئد

5 دانشیار گروه تولید و بهره‌برداری آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران

6 استاد دانشگاه گرنوبل فرانسه

چکیده

تشخیص ظاهری ماهی آزاد دریای خزر، (Salmo caspius (Kessleri, 1877 و ماهی قزل آلای رنگین کمان (Onchorhyncus mykiss (Walbaum 1792 که ممکن است از قفس های پرورشی مستقر در سواحل جنوبی دریای خزر و یا مراکز تکثیر فرار کرده و به دریا راه یافته باشند، بسیار دشوار است. در این مطالعه، تعداد 20 ماهی از مناطق مختلف دریای خزر: بندر انزلی (3 ماهی آزاد)، تنکابن (4 ماهی آزاد، 1 ماهی قزل آلای رنگین کمان)، نوشهر (1 ماهی آزاد، 1 ماهی قزل آلای رنگین کمان)، فریدونکنار (3 ماهی آزاد)، ساری (1 ماهی آزاد، 1 ماهی قزل آلای رنگین کمان) و میانکاله (3 ماهی آزاد، 1 ماهی قزل آلای رنگین کمان) صید و با استفاده از توالی یابی ژنومی تا حد امکان این دو گونه را از هم تفکیک و شناسایی کرد. نتایج اولیه توالی یابی ژنوم میتوکندریایی درصد نزدیکی ژنوم توالی یافته با گونه های ثبت شده در GenBank را نشان داد. نمونه های صید شده در میانکاله، ساری و فریدونکنار و انزلی با ظاهری بسیار شبیه به ماهی آزاد، در توالی یابی شباهت بالای ژنومی را با ماهی قزل الا رنگین کمان نشان دادند. بقیه نمونه ها با ظاهر ماهی آزاد همان توالی ماهی آزاد را نشان دادند. بر اساس این مطالعه، احتمال فرار قزل آلا از قفس های پرورشی وجود دارد که می‌تواند اثرات زیادی بر اکوسیستم منطقه و آبزیان از جمله گونه های دیگر ماهی بگذارد.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of Caspian salmon (Salmo caspius (Kessleri, 1877) and rainbow trout (Onchorhyncus mykiss (Walbaum 1792) escaping from breeding cages using Cyt b gene sequencing in the southern Caspian basin

نویسندگان [English]

  • Seyede Sara Jafari Kenari 1
  • Rasoul Ghorbani 2
  • Hamid Reza Rezaie 3
  • Rahmatollah Naddafi 4
  • Hadiseh Kashiri 5
  • Francois Pompanon 6
1 Ph.D. Student of Aquatic Production and Exploitation, Gorgan University of Agricultural Sciences and Natural Resources, Iran
2 Corresponding Author, Professor, Dept. of Aquatic Production and Exploitation, Gorgan University of Agricultural Sciences and Natural Resources, Iran.
3 Associate Prof., Dept. of Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Iran
4 Associate Prof., Dept. of Aquatic Resources, Dept. of Coastal Research, Uppsala University of Agricultural Sciences, Sweden
5 Associate Prof., Dept. of Aquatic Production and Exploitation, Gorgan University of Agricultural Sciences and Natural Resources
6 Professor at the University of Grenoble, France
چکیده [English]

Appearance identification of Caspian salmon (Salmo caspius (Kessleri, 1877) and rainbow trout (Onchorhyncus mykiss (Walbaum 1792), which may have escaped from breeding cages on the southern shores of the Caspian Sea or breeding grounds and into the sea. In this study, 20 fish from different regions of the Caspian Sea: Bandar Anzali (3 salmon), Tonekabon (4 salmon, 1 rainbow trout), Nowshahr (1 salmon, 1 salmon) Rainbow mite), Fereydunkenar (3 salmon), Sari (1 salmon, 1 rainbow trout) and Miankaleh (3 salmon, 1 rainbow trout) were caught and used as much as possible using genomic sequencing He distinguished and identified these two species. Some tail fins were isolated from all samples and fixed in 96% alcohol for DNA extraction and Bioneer extraction column kit was used for DNA extraction. Preliminary results of mitochondrial genome sequencing showed the proximity of the sequenced genome to the species registered in GenBank. Specimens caught in Miankaleh, Sari, Fereydunkenar and Anzali with a very similar appearance to salmon showed high genomic similarity with rainbow trout in sequencing. The rest of the samples showed the same salmon sequence with the appearance of salmon. According to this study, there is a possibility of salmon escaping from breeding cages, which can have a great impact on the ecosystem of the region and aquatic animals, including other species of fish.

کلیدواژه‌ها [English]

  • Keywords: Caspian Sea salmon
  • rainbow trout
  • Caspian Sea
  • cage
  • genetic sequencing

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مجله بهره برداری و پرورش آبزیان
دوره 11، شماره 1
فروردین 1401
صفحه 1-16

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