اثرات استفاده مجزا و تلفیقی اسید فرمیک و پروبیوتیک پدیوکوکوس اسید لاکتیکی (Pediococcus acidilactici ) بر شاخص‌های رشد و مقاومت به شوری در بچه ماهی کپور معمولی(Cyprinus carpio)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 مؤسسه تحقیقات علوم شیلاتی کشور

چکیده

در این آزمایش اثر پروبیوتیک پدیوکوکوس اسید لاکتیکی (Pediococcus acidilactici)، اسید فرمیک و تلفیق پروبیوتیک و اسید فرمیک بر شاخص‌های رشد و مقاومت به شوری در ماهی کپور معمولی (Cyprinus carpio) مورد بررسی قرار گرفت. بدین منظور تعداد 228 قطعه ماهی کپور با میانگین وزنی 5/0±5/5 گرم به مدت 8 هفته با 3 جیره‌ آزمایشی حاوی 1/0 درصد پروبیوتیک پدیوکوکوس اسید لاکتیکی، 3/0 درصد اسید فرمیک و تلفیق پروبیوتیک و اسید فرمیک و جیره شاهد (بدون افزودنی) با 3 تکرار تغذیه شدند. در پایان دوره شاخص‌های رشد و مقاومت به شوری در تیمارهای مختلف مورد بررسی قرار گرفتند. نتایج نشان داد در شاخص میانگین وزن ثانویه و افزایش وزن اختلاف معنی‌داری در تیمار تلفیقی و همچنین تیمار مجزای اسید فرمیک با تیمار پروبیوتیک و شاهد مشاهده شد. نرخ رشد ویژه و درصد بازماندگی اختلاف معنی‌داری بین تیمارها نشان نداد (05/0P>). کمترین میزان ضریب تبدیل غذایی در تیمارهای تلفیقی پروبیوتیک و اسید فرمیک مشاهده شد. درصد بازماندگی در شوری 15 گرم در هزار در ماهیان تغذیه شده با تیمارهای غذایی مختلف طی 7 روز در تیمار تلفیقی در تمام روزهای مواجهه با تنش شوری بیشترین میزان (100 درصد) و در تیمار پروبیوتیک بیشتر از تیمار اسید فرمیک و شاهد بود. نتایج این مطالعه نشان‌دهنده‌ی اثرات مفید پدیوکوکوس اسید لاکتیکی و اسید فرمیک به ویژه به‌‌صورت تلفیقی بر شاخص‌های رشد و مقاومت به شوری در ماهی کپور می‌باشد

کلیدواژه‌ها


عنوان مقاله [English]

Effects of singular or combined administration of formic acid and Pediococcus acidilactici on growth indices and resistance to salinity in common carp fingerling (Cyprinus carpio)

نویسندگان [English]

  • fatemeh heshmatfar 1
  • Roghayeh Safari 1
  • Ali Shabany 1
  • Hosein Hoseinifar 1
  • Hadi Ghafari 2
1 Gorgan University of Agricultural Sciences and Natural Resources
2 National Institute of Fisheries Research
چکیده [English]

In this experiment effects of singular or combined administration of formic acid and Pediococcus acidilactici on growth indices and resistance to salinity in common carp fingerling (Cyprinus carpio) were investigated. For this purpose 228 fish with average weight of 5.5±0.5 gr, were fed diets supplemented for 8 weeks using experimental diet including probiotic (0.1%), formic acid (0.3%), combination of probiotic and formic acid and a control group. At the end of feeding trial growth indices and resictance to salinity were evaluated. Significant differences (P≤0.05) were observed in final weight and weight gain in fish fed combined probiotic and formic acid compare with singular probiotic and control group. SGR and survival percentage showed no significant difference between treatments (P≥0.05). The least FCR were observed in fish fed combined probiotic and formic acid (p < 0.05). The results of this experiment showed suitable effect of using probiotic and formic acid espsially in combination form on growth and resistance to salinity in Common carp.

کلیدواژه‌ها [English]

  • Cyprimus carpio
  • Probiotic
  • Organic acid
  • formic acid
  • growth
1.Abu Elala, N.M., and Ragaa, N.M. 2015. Eubiotic effect of a dietary acidifier (potassium diformate) on the health status of cultured (Oreochromis niloticus) J. Adv. Res. 6: 621-629.
2.Adeoye, A.A., Yomla, R., Jaramillo-Torres, A., Rodiles, A., Merrifield, D.L., and Davies, S.J. 2016. Combined effects of exogenous enzymes and probiotic on Nile tilapia (Oreochromis niloticus) growth, intestinal morphology and microbiome. Aquaculture. 463: 61-70.
3.Baruah, K., Sahu, N.P., Pal, A.K., Debnath, D., Yengkokpam, S., and Mukherjee, S.C. 2007b. Interactions of dietary microbial phytase, citric acid and crude protein level on mineral utilization by rohu (Labeo rohita), juveniles. World Aquaculture Society. 38: 238-249.
4.Carnevali, O., Cardinali, M., Maradonna, F., Parisi, M., Olivotto, I., Polzonetti‐Magni, A.M., and Funkenstein, B. 2005. Hormonal regulation of hepatic IGF‐I and IGF‐II gene expression in the marine teleost Sparus aurata. Molecular reproduction and development. 71: 1. 12-18.
5.Dai, J., Li, Y., Yang, P., Liu, Y., and Chen, Z. 2018. Citric acid as a functional supplement in diets for juvenile turbot, (Scophthalmus maximus): Effects on phosphorus discharge, growth performance, and intestinal health. Aquacalture. 1: 1-10.
6.Freitag, M. 2007. Organic acids and salts promote performance and health in animal husbandry. In: Luckstadt, C., editor. Acidifiers in Animal Nutrition – A Guide for Feed Preservation and Acidification to Promote Animal Performance. 1st ed, Nottingham University Press, Nottingham, UK. Pp: 1-11.
7.Ghosh, S., Sinha, A., and Sahu, C. 2007. Effect of probiotic on reproductive performance in female livebearingornamental fish. Aquaculture Research. 38: 5. 518-526.
8.Hossain, M.A., Pandey, A., and Satoh, S. 2007. Effects of organic acids on growth and phosphorus utilization in red sea bream Pagrus major. Fisheries science. 73: 1309-1317.
9.Hoseinifar, S.H., and Romano, N. 2017. Comparing the effects of different dietary organic acids on the growth, intestinal short-chain fatty acids, and liver histopathology of red hybrid tilapia (Oreochromis sp.) and potential use of these as preservatives. Fish Physiology and Biochemistry. 11: 1-13.
10.Hu, J., Ran, C., He, S., Cao, Y., Yao,B., Ye, Y., and Zhou, Z. 2016.Dietary microbial phytase exerts mixed effects on the gut health of tilapia: a possible reason for the null effecton growth promotion. British J. Nutr.115: 11. 1958-1966.
11.Khajepour, F., and Hosseini, S.A. 2012. Citric acid improves growth performance and phosphorus digestibility in Beluga (Huso huso) fed diets where soybean meal partly replaced fish meal. Animal Feed Science and Technology. 171: 68-73.
12.Lim, C., Klesius, P.H., Li, M.H., and Robinson, E.H. 2000. Interaction between dietary levels of iron and vitamin C on growth, hematology, immune response and resistance of channel catfish (Ictalurus punctatus)to Edwardsiella ictaluri challenge. Aquaculture. 185: 313-327.
13.Luckstadt, C. 2008. The use of acidifiers in fish nutrition. CAB Reviews: Perspectives in Agriculture, Veterinary Science. Nutrition and Natural Resources. 3: 44. 1-8.
14.Munir, M.B., Hashim, R., Manaf, M.S.A., and Nor, S.A.M. 2016. Dietary prebiotics and probiotics influence the growth performance, feed utilisation, and body indices of snakehead(Channa striata) fingerlings. Tropical life sciences research. 27: 2. 111.
15.Marshall, W.S., and Singer, T.D. 2002. Cystic fibrosis transmembrane conductance regulator in teleost fish. Biochimica et Biophysica Acta.1566: 16-27.
16.Nayak, S.K. 2010. Probiotics and immunity: a fish perspective. Fish and shellfish immunology. 29: 1. 2-14.
17.Ng, W.K., Koh, C.B., Sudesh, K., and Siti-Zahrah, A. 2009. Effects of dietary organic acids on growth, nutrient digestibility and gut microflora of red hybrid tilapia, (Oreochromis sp) and subsequent survival during a challenge test with (Streptococcus agalactiae). Aquaculture Research. 40: 1490-1500.
18.Owen, M.A.G., Waines, P., Bradley, G., and Davies, S. 2006. The effect of dietary supplementation of sodium butyrate on the growth and microflora of (Clarias gariepinus) (Burchell 1822). Abstract from the 12th International Symposium Fish Nutrition and Feeding. May 28-June 1, 2006, Biarritz, France.
19.Pandey, A., and Satoh, S. 2008. Effects of organic-acids on growth and phosphorus utilization in rainbow
trout (Oncorhynchus mykiss). Fisheries science. 74: 867-874.
20.Ringo, E. 1991. Effects of dietary lactate and propionate on growth and digesta in Arctic charr, (Salvelinus alpinus). Aquaculture. 96: 321-333.
21.Romano, N., Koh, C.B., and Ng,W.K. 2015. Dietary microencapsulated organic acids blend enhances growth, phosphorus utilization, immune response, hepatopancreatic integrity and resistance against Vibrio harveyi in white shrimp, Litopenaeus vannamei. Aquaculture. 435: 228-236.
22.Salze, G., Mclean, E., Schwarz, M.H., and Craig, S.R. 2008. Dietary mannan oligosaccharide enhances salinity tolerance and gut development of larval cobia. Aquaculture, 274: 148-150.
23.Safari, R., Hoseinifar, S.H., and Kavandi, M. 2016. Modulation of antioxidant defense and immune response in zebra fish (Danio rerio) using dietary sodium propionate. Fish Physiology and Biochemistry. 10: 10-20.
24.Safari, R., Hoseinifar, S.H.., Nejadmoghadam, S.H., and Khalil, M. 2017. Non-specific immune parameters, immune, antioxidantand growth-related genes expression of common carp (Cyprinus carpio) fed sodium propionate. Aquaculture Research.5: 1-9.
25.Sarker, M.S.A., Satoh, S., and Kiron, V. 2007. Inclusion of citric acid and/or acid-chelated trace elements in alternate plant protein source diets aspects growth and excretion ofnitrogen and phosphorus in red sea bream (Pagrus major). Aquaculture. 262: 436-443.
26.Sodagar, M., Hosseinpoor, Z., and Hosseini. A. 2010. The use of citric acid as attractant in diet of grand sturgeon (Hoso hoso) fry and its effects on growing factors and survival rate. Aquacalture, Aquarium, Cons. Legis. Inter. J. Bioflux Soc. 3: 4. 311-316.
27.Standen, B.T., Rawling, M.D., Davies, S.J., Castex, M., Foey, A., Gioacchini, G., and Merrifield, D.L. 2013. Probiotic Pediococcus acidilactici modulatesboth localised intestinal-and peripheral-immunity in tilapia (Oreochromis niloticus). Fish & shellfish immunology, 35: 4. 1097-1104.
28.Taoka, Y., Maeda, H., Jo, J.Y.,Jeon, M.J., Bai, S.C., Lee, W.J.,Yuge, K., and Koshio, S. 2006. Growth, stress tolerance and non-specific immune response of Japanese flounder (Paralichthys olivaceus) to probiotics in a closed recirculating system. Fisheries Science. 72: 310-321.
29.Taridashti, F., Delafkar, K., Zare, A., and Azari-Takami, G. 2017. Effects of probiotic Pediococcus acidilactici on growth performance, survival rate, and stress resistance of Persian sturgeon (Acipenser persicus). J. Appl. Aquacul. 29: 3-4. 220-232.
30.Zhou, Y., Yuan, X., Liang, X.F., Fang, L., Li, J., Guo, X., and He, S.2013. Enhancement of growth and intestinal flora in grass carp: the effect of exogenous cellulase. Aquaculture. 416: 1-7.