تاثیر دماهای مختلف بر میزان ذخیره چربی جلبک Dunaliella sp.

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسنده

بوم شناسی آبزیان، دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی، گرگان، ایران

چکیده

علم بیوتکنولوژی به دنبال یافتن محرک‌هایی موثر جهت افزایش نرخ رشد و محتوای ترکیبات مختلف بیوشیمیایی مانند لیپیدها و رنگدانه‌ها در جلبک‌هاست. دما یکی از مهم‌ترین فاکتورهای فیزیکی است که تاثیر قابل توجهی بر رشد و فعالیت موجودات آبزی دارد. دونالیلا جلبکی تک‌سلولی است که در رده کلروفیسه‌ها طبقه‌بندی می‌شود، این جلبک بدون دیواره سلولی، متحرک و شوری پسند است. جلبک دو تاژکی دونالیلا به‌عنوان مقاوم‌ترین ارگانیسم یوکاریوت فتوسنتز کننده شناخته شده است. بیشتر جلبک‌های میکروسکوپی تحت شرایط استرس مقادیر بالایی از لیپید تولید می‌کنند. تغییر شرایط محیطی علاوه بر تاثیر بر میزان رشد و تولید چربی می‌تواند بر کیفیت چربی ریزجلبک‌ها نیز اثر‌گذار باشد. در این تحقیق جلبک دونالیلا پس از نمونه برداری از دریاچه ارومیه و خالص‌سازی، در محیط کشت والن کشت داده شد. آزمایش با 4 تیمار و 6 تکرار به طور کاملا تصادفی انجام شد. برای رشد مطلوب جلبک دونالیلا، شدت نور 3000 لوکس با 12 ساعت دوره روشنایی و 12 ساعت دوره تاریکی و محیط کشت والن فراهم گردید. شمارش تعداد سلول جلبک و اندازه‌گیری دما و پی اچ روزانه و میزان چربی در فازهای مختلف رشد صورت گرفت. تجزیه و تحلیل داده ها با استفاده از نرم افزار آماری SPSS انجام گرفت. با توجه به نتایج بدست آمده رشد جلبک با افزایش دما کاهش یافت و بیشترین رشد در دمای 20 درجه سانتی‌گراد مشاهده گردید همچنین به این نتیجه دست یافتیم که بیشترین میزان ذخیره چربی در درمای 25 درجه سانتی‌گراد و در فاز ثابت بود.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of different temperatures on amount of fat storage of Dunaliella sp.

نویسنده [English]

  • fatemeh ayatolahi
ecology,shilat,university of agricultureal sience and natural resources,gorgan,iran
چکیده [English]

Biotechnology seeks to find effective stimuli to increase the rate of growth and the content of various biochemical compounds such as lipids and pigments in algae. Temperature is one of the most important physical factors that has a significant impact on the growth and activity of aquatic organisms. Dunaliella is an algae that is categorized as chlorophyll in algae without cell walls, moving, and salty. Dunaliella two-tagged algae are recognized as the most resistant photosynthetic eukaryotic organism. Most microscopic algae produce high levels of lipids under stress conditions. Changing environmental conditions, in addition to affecting the amount of growth and fat production, can also affect the quality of the microalgae fat. In this research Dunaliella algae were cultured in Whalen culture medium after sampling from Uromiye Lake and purification. Experiments were performed with 4 treatments and 6 replicates completely randomized. For the optimal growth of Dunaliella algae, the lightness of 3000 lux was achieved with 12 hours of lightning and 12 hours of darkness and Whalen's culture medium. Counts of algae cells, daily temperature and pH measurements, and fat content in different growth phases. Data were analyzed using SPSS software. According to the results, the growth of algae decreased with increasing temperature and the highest growth was observed at 20 ° C. Also, we found that the highest amount of fat storage was in the droplet at 25 ° C and in the constant phase.

کلیدواژه‌ها [English]

  • Microalgae
  • Temperature
  • Fat
  • Dunaliella
  • Growth

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مجله بهره برداری و پرورش آبزیان
دوره 8، شماره 4
اسفند 1398
صفحه 11-20

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