ارزیابی فعالیت ضدباکتریایی و مقایسه فراسنجه های ایمنی موکوس پوست و پلاسمای تاس ماهی ایرانی(Acipenser persicus) و روسی (Acipenser gueldenstaedtii)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 گروه تکثیر و پرورش آبزیان ، دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 گروه ژنتیک و بیوتکنولوزی ، دانشگاه صنعتی اصفهان

چکیده

تحقیق حاضر با هدف مقایسه فراسنجه های ایمنی موکوس پوست تاس ماهی ایرانی (Acipenser persicus) و تاس ماهی روسی (Acipenser gueldenstaedtii) انجام شد. از تعداد 20 عدد ماهی (10 عدد از هر گونه) نمونه برداری موکوس و پلاسما صورت گرفت و فاکتورهای پروتئین محلول، لیزوزیم، فسفاتاز قلیایی و ایمونوگلوبولین کل در موکوس و پلاسمای دو گونه اندازه گیری شد. نتایج نشان داد که میزان پروتئین محلول(گرم بر دسی لیتر) ، ایمنوگلوبولین کل(میلی گرم بر میلی لیتر) ، آلکالین فسفاتاز(واحدبین المللی در میلی لیتر) و لیزوزیم(واحدبین المللی در میلی لیتر) موکوس و پلاسما در ماهی قره برون به ترتیب 1.11±7.6 ، 0.26±8.46 ، 1.4±23 ،1.6±38 ، 1.4±23 ، 8.77±134.06 ، 1.3±24.8 و 16.97±85 و در ماهی چالباش به ترتیب 0.26±8.46 ، 0.75± 9.71 ، 2.4± 19.4 ، 36.6±1.4 ، 23.7±2.54 ، 83.70±19.68 ، 13.61±0.8 و 49.5±14.85 می باشد. میزان پروتئین محلول و ایمنوگلوبولین کل در هر دو نمونه موکوس و پلاسما و میزان آلکالین فسفاتاز بین موکوس دو گونه باهم تفاوت معنی داری نداشند.(P> 0.05) در حالیکه مقدار لیزوزیم در موکوس و پلاسما و میزان آلکالین فسفاتاز در نمونه های پلاسمای دو گونه باهم اختلاف معنی دار نشان دادند (P<0.05). همچنین بر اساس نتایج موجود هیچ فعالیت ضد باکتریایی در موکوس و پلاسمای دو ماهی چالباش و قره برون مشاهده نشد. به طور کلی به نظر می رسد که موکوس و پلاسما به عنوان یکی از اجزای مرتبط در ایمنی غیر اختصاصی به شمار می رود و در تحقیق حاضر سطح پارامتر های ایمنی در قره برون بیشتر است .

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of antibacterial activity and immune parameters of skin mucus, plasma in Persian (Acipenser persicus) and Russian sturgeon (Acipenser gueldenstaedtii)

نویسندگان [English]

  • Hamed Azadi 1
  • Hamed Kolangi Miandare 1
  • Abdolmajid Hajimoradloo 1
  • Mahdi Abbasian 2
1 Gorgan University of Agricultural Sciences &amp;amp; Natural Resources
2
چکیده [English]

the present study aimed to compare immune parameres of skin mucus and plasma in Persian sturgeon and Russian sturgeon. The mucus and plasma samples was collected from 20 specimens (10 Persian and 10 Russian sturgeon). Total protein, lysozyme, alkaline phosphatase (ALP) and total immunoglobulins of the samples were measured. The levels of Total protein, immunoglobulin, alkaline phosphatase of Persian sturgeon in plasma and mucus were, 8.46 ± 0.26, 7.6 ± 1.111, 38± 1.6, 23± 1.4, 134.06 ± 8.77 and 23± 1.4, respectively. The levels of Total protein, immunoglobulin, alkaline phosphatase of Russian sturgeon in plasma and mucus were, 9.71 ± 0.75, 8.2 ± 1.40, 36.6 ± 1.4, 19.8 ± 2.4, 83.70 ± 19.68 and 23.17 ± 2.54, respectively. Results showed that the levels of Total proteinand total immunoglobulinin both samples of mucus and plasma and levels of alkaline phosphatase in mucus were not significantly differ between the two species(P> 0.05), While the levels of lysozyme in mucus and plasma and alkaline phosphatase levels in plasma samples were recorded significant differences Between two species (P <0.05). Also no antibacterial activity in mucus and plasma of two species were recorded

کلیدواژه‌ها [English]

  • "Non-specific immunity"
  • "antibacterial activity"
  • "sturgeons"
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