اثر مسمومیت تحت کشنده حشره‌کش دانیتول بر فعالیت آنزیم‌های آنتی اکسیدانی گلبول‌های قرمز ماهی کپور معمولی (Cyprinus carpio)

نوع مقاله: مقاله علمی - پژوهشی

نویسنده

عضو هیئت علمی. مرکز تحقیقات ذخایر آبزیان آبهای داخلی-گرگان

چکیده

دانیتول یک حشره‌کش غیر سیستمیک است که در کشاورزی استفاده می‌شود. با توجه به مطالعات معدود در خصوص سمیت دانیتول در ماهی‌ها، هدف از این تحقیق تعیین سمیت حشره‌کش دانیتول در ماهی کپور معمولی و اثر آن بر فعالیت آنزیم‌های آنتی اکسیدان گلبول‌های قرمز بود. به این منظور، ماهیان به مدت 96 ساعت در معرض غلظت‌های صفر، 2/0، 5/0، 1، 5/1 و 2 میلی گرم بر لیتر دانیتول قرار گرفتند و غلظت کشنده دانیتول با استفاده از داده‌های تلفات 47/0 میلی گرم بر لیتر محاسبه شد. سپس، جهت بررسی اثر مسمومیت تحت کشنده دانیتول بر فعالیت آنزیم‌های آنتی اکسیدان گلبول‌های قرمز، ماهیان به مدت 21 روز در معرض 05/0 و 1/0 میلی گرم بر لیتر دانیتول قرار گرفتند. در پایان دوره، از تیمارهای مختلف نمونه خون گرفته و جهت بررسی آنزیم‌های آنتی اکسیدان استفاده شد. نتایج نشان داد که دانیتول یک ماده "بسیار سمی" برای ماهی کپور معمولی است. همچنین، مسمومیت با دانیتول باعث کاهش فعالیت آنزیم‌های سوپرکسید دیسموتاز، کاتالاز، گلوتاتیون پرکسیداز و گلوتاتیون ردوکتاز می‌شود ولی این کاهش معنی‌دار نبود. همبستگی منفی و معنی‌داری بین غلظت دانیتول و فعالیت آنزیم‌های آنتی اکسیدان وجود داشت. در نهایت می‌توان گفت مسمومیت تحت کشنده با دانیتول باعث بروز استرس اکسیداتیو در ماهی کپور می‌شود و اندازه‌گیری آنزیم‌های آنتی اکسیدان می‌تواند شاخص مناسبی برای بررسی آن باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Sub-lethal effect of pesticide danitol on erythrocyte antioxidant enzymes activity of common carp (Cyprinus carpio)

چکیده [English]

Danitol is a non-systemic insecticide, which is used in agriculture. Considering the rare data on danitol toxicity in fish, the aim of this study was to investigate pesticide danitol toxicity in common carp and its effects on erythrocyte antioxidant enzymes activity. For this purpose, the fish were exposed to 0, 0.2, 0.5, 1, 1.5 and 2 mg/l danitol for 96 h; and danitol acute toxicity was found to be 0.47 mg/l according to mortality data. Then, the fish were exposed to 0.05 and 0.1 mg/l danitol for 21 days to investigate chronic effects of danitol on the fish erythrocyte antioxidant enzymes. At the end of the trial, blood samples were taken from different treatments and used for antioxidant enzymes’ activity determination. The results showed that danitol is a “very toxic” substance in common carp. Also, danitiol toxicity decreased superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activity, but not significantly. There was a negative and significant relationship between danitol concentration and antioxidant enzymes’ activity. Overall, it is suggested that danitol toxicity leads oxidative stress in common carp and determination of antioxidant enzymes’ activity is a suitable indicator to monitor the stress.

کلیدواژه‌ها [English]

  • pollutant
  • Pesticide
  • Blood
  • physiology
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