نوع مقاله : مقاله کامل علمی - پژوهشی
نویسندگان
1 دانشگاه تربیت مدرس
2 دانشگاه تربیت مدرس گروه میکروب شناسی پزشکی
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Cell lines provide an important biological tool for carrying out investigations into physiology, virology, pharmacology, toxicology, cancer which can be used as a biological substitute for living animals. Cell phenomena are studied in controlled conditions in cell culture. Establishment of cell cultures from endemic fish and endangered Such as the Caspian Sea salmon (Salmo caspius, Kessler, 1877). Production in vitro models of it can be of assistance to biological studies, immunology, toxicology, physiology. The purpose of this study is to produce primary cell cultures from the kidney and gill of Caspian salmon in order to create cell lines from these tissues in the next stages. In this study, 30 Caspian Sea salmon (Salmo caspius, Kessler, 1877) prepared and primary cell was produced from kidney and gill tissues through the explant culture method. Kidney and gill tissue pieces were cultured in 6-well plates with L-15 culture medium and 5%, 10% and 20% fetal bovine serum (FBS) at 15, 18° and 21C. The results showed that primary cells produced from Caspian Sea salmon are slow to grow and take a long time for cell migration from the tissue and doubling time. But they have a relatively good subculture and grow well up to passage 5 in L-15 culture medium with 10% fetal bovine serum. The morphological study of the produced cells showed that they are a mixture of fibroblast-like and epithelial-like cells. Investigating the process of cell growth at different temperatures showed that the optimal growth temperature for the cultivation of cells of this species is 18 C.
کلیدواژهها [English]
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