تاثیر استفاده از سین بیوتیک پکتین و لاکتوباسیلوس پلانتاروم بر عملکرد رشد و بیان ژن‌های مرتبط با رشد بچه فیل ماهی (Huso huso)

نوع مقاله : مقاله کامل علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری گروه تکثیر و پرورش آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

2 نویسنده مسئول، دانشیار گروه تکثیر و پرورش آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 دانشیار گروه تکثیر و پرورش آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

4 استاد گروه تکثیر و پرورش آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

چکیده

به همین منظور هدف از این مطالعه بررسی اثر پکتین و لاکتوباسیلوس پلانتاروم بر فاکتورهای رشد و بیان ژن‌های GH و IGF فیل ماهی می‌باشد. به‌همین منظور تعداد 120 بچه فیل ماهی با میانگین وزنی 3±15 گرم به‌صورت تصادفی در تانک‌های 400 لیتری توزیع شدند. تیمارها شامل شاهد، جیره حاوی پکتین (1درصد)، پکتین (2درصد)، پروبیوتیک L. plantarum (cfu g-1 107)، پروبیوتیک L. plantarum (cfu g-1 108)، ترکیب پروبیوتیک L. plantarum (cfu g-1 107) و پکتین (1 درصد)، ترکیب پروبیوتیک L. plantarum (cfu g-1 108) و پکتین (1 درصد)، ترکیب پروبیوتیک L. plantarum (cfu g-1 107) و پکتین (2 درصد) و ترکیب پروبیوتیک L. plantarum (cfu g-1 108) و پکتین (2 درصد) بودند. پس از 8 هفته تغذیه زیست‌سنجی به‌منظور ارزیابی فاکتورهای رشد انجام شد و جهت ارزیابی بیان ژن نمونه‌برداری از مغز و کبد انجام شد. در پایان دوره آزمایش نتایج نشان داد که اگرچه استفاده از پکتین و لاکتوباسیلوس پلانتاروم به‌صورت منفرد در جیره باعث افزایش عملکرد رشد شد اما بالاترین میزان وزن نهایی، افزایش وزن و نرخ رشد ویژه و کمترین میزان ضریب تبدیل غذایی در ماهیان تغذیه شده با سطح 2 درصد پکتین و cfu g-1 108 بود که اختلاف معنی‌داری با سایر تیمارها و گروه شاهد داشت (P<0.05). همچنین بیشترین سطح بیان ژن‌های GH و IGF نیز در این تیمار مشاهده شد و کمترین سطح بیان در گروه شاهد بود. به‌طورکلی ترکیب پکتین و لاکتوباسیلوس پلانتاروم به‌ترتیب 2 درصد و cfu g-1 108در جیره دارای اثرات مثبت و معنی‌داری روی عملکرد رشد بچه فیل ماهی می‌باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of using synbiotic pectin and Lactobacillus plantarum on growth performance and expression of genes related to growth in beluga sturgeon (Huso huso)

نویسندگان [English]

  • Esmaeil Jabbari 1
  • Valiollah Jafari 2
  • Roghayeh Safari 3
  • Seyed Hossein Hoseinifar 3
  • Mohammadreza Imanpour 4
1 Ph.D. Student, Dept. of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Corresponding Author, Associate Prof., Dept. of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
3 Associate Prof., Dept. of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Professor, Dept. of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
چکیده [English]

Increasing the growth and immune system of beluga sturgeon (Huso huso) which is a valuable species is an important aim for aquaculture so The purpose of this study was to investigate the effect of pectin and L. plantarum on growth performance and growth genes of juvenile beluga sturgeon (Huso huso). Triplicate groups of beluga juveniles each (15 ± 3 g) were placed in 9 oval tanks (400) and fed with the 107 cfu g-1, 108 cfu g-1, pectin (1%), pectin (2%), 107 cfu g-1 and 1% pectin, 107 cfu g-1 and 2% pectin, 108 cfu g-1 and 1% pectin, 108 cfu g-1 and 2% pectin and control group. The trial lasted 8 weeks. At the end of experiment biometery was done and brain and liver tissue were separataed. Results showed that although pectin and L. plantarum could enhance growth factors separately,the highest FW, GW and SGR was observed in fish fed 108 cfu g-1 and 2% pectin which had significant differences with others treatments and control (P<0.05). Also the highest genes expression was in this treatment and the lowest in control. In general, pectin and L. plantarum could significantly affect growth performance in juvenile blugae

کلیدواژه‌ها [English]

  • Probiotic
  • Prebiotib
  • growth performance and gene expression

مراجع

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مجله بهره برداری و پرورش آبزیان
دوره 12، شماره 2
تیر 1402
صفحه 1-12

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