ارزیابی فعالیت ضدباکتریایی و مقایسه فراسنجه های ایمنی موکوس پوست و پلاسمای تاس ماهی ایرانی(Acipenser persicus) و روسی (Acipenser gueldenstaedtii)

نوع مقاله: مقاله علمی - پژوهشی

نویسندگان

1 گروه تکثیر و پرورش آبزیان ، دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 گروه ژنتیک و بیوتکنولوزی ، دانشگاه صنعتی اصفهان

چکیده

تحقیق حاضر با هدف مقایسه فراسنجه های ایمنی موکوس پوست تاس ماهی ایرانی (Acipenser persicus) و تاس ماهی روسی (Acipenser gueldenstaedtii) انجام شد. از تعداد 20 عدد ماهی (10 عدد از هر گونه) نمونه برداری موکوس و پلاسما صورت گرفت و فاکتورهای پروتئین محلول، لیزوزیم، فسفاتاز قلیایی و ایمونوگلوبولین کل در موکوس و پلاسمای دو گونه اندازه گیری شد. نتایج نشان داد که میزان پروتئین محلول(گرم بر دسی لیتر) ، ایمنوگلوبولین کل(میلی گرم بر میلی لیتر) ، آلکالین فسفاتاز(واحدبین المللی در میلی لیتر) و لیزوزیم(واحدبین المللی در میلی لیتر) موکوس و پلاسما در ماهی قره برون به ترتیب 1.11±7.6 ، 0.26±8.46 ، 1.4±23 ،1.6±38 ، 1.4±23 ، 8.77±134.06 ، 1.3±24.8 و 16.97±85 و در ماهی چالباش به ترتیب 0.26±8.46 ، 0.75± 9.71 ، 2.4± 19.4 ، 36.6±1.4 ، 23.7±2.54 ، 83.70±19.68 ، 13.61±0.8 و 49.5±14.85 می باشد. میزان پروتئین محلول و ایمنوگلوبولین کل در هر دو نمونه موکوس و پلاسما و میزان آلکالین فسفاتاز بین موکوس دو گونه باهم تفاوت معنی داری نداشند.(P> 0.05) در حالیکه مقدار لیزوزیم در موکوس و پلاسما و میزان آلکالین فسفاتاز در نمونه های پلاسمای دو گونه باهم اختلاف معنی دار نشان دادند (P<0.05). همچنین بر اساس نتایج موجود هیچ فعالیت ضد باکتریایی در موکوس و پلاسمای دو ماهی چالباش و قره برون مشاهده نشد. به طور کلی به نظر می رسد که موکوس و پلاسما به عنوان یکی از اجزای مرتبط در ایمنی غیر اختصاصی به شمار می رود و در تحقیق حاضر سطح پارامتر های ایمنی در قره برون بیشتر است .

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of antibacterial activity and immune parameters of skin mucus, plasma in Persian (Acipenser persicus) and Russian sturgeon (Acipenser gueldenstaedtii)

نویسندگان [English]

  • Hamed Azadi 1
  • Hamed Kolangi Miandare 1
  • Abdolmajid Hajimoradloo 1
  • Mahdi Abbasian 2
1 Gorgan University of Agricultural Sciences &amp;amp; Natural Resources
چکیده [English]

the present study aimed to compare immune parameres of skin mucus and plasma in Persian sturgeon and Russian sturgeon. The mucus and plasma samples was collected from 20 specimens (10 Persian and 10 Russian sturgeon). Total protein, lysozyme, alkaline phosphatase (ALP) and total immunoglobulins of the samples were measured. The levels of Total protein, immunoglobulin, alkaline phosphatase of Persian sturgeon in plasma and mucus were, 8.46 ± 0.26, 7.6 ± 1.111, 38± 1.6, 23± 1.4, 134.06 ± 8.77 and 23± 1.4, respectively. The levels of Total protein, immunoglobulin, alkaline phosphatase of Russian sturgeon in plasma and mucus were, 9.71 ± 0.75, 8.2 ± 1.40, 36.6 ± 1.4, 19.8 ± 2.4, 83.70 ± 19.68 and 23.17 ± 2.54, respectively. Results showed that the levels of Total proteinand total immunoglobulinin both samples of mucus and plasma and levels of alkaline phosphatase in mucus were not significantly differ between the two species(P> 0.05), While the levels of lysozyme in mucus and plasma and alkaline phosphatase levels in plasma samples were recorded significant differences Between two species (P <0.05). Also no antibacterial activity in mucus and plasma of two species were recorded

کلیدواژه‌ها [English]

  • "Non-specific immunity"
  • "antibacterial activity"
  • "sturgeons"
Chebanov, M.S., Galich, Chebanov, E.V., Galich, Sturgeon hatchery manual. FAO
Fisheries and Aquaculture Technical Paper. 558, Ankara, FAO, 2013.
Ebran, N., Julien, S., Orange, N., Saglio, P., Lemaı̂tre, C., and Molle, G. 1999.
Pore-forming properties and antibacterial activity of proteins extracted from
epidermal mucus of fish. Comparative Biochemistry and Physiology Part A:
Molecular and Integrative Physiology, 122(2): 181-189.
Ellis, A.E. 2001. Innate host defense mechanisms of fish against viruses and
bacteria. Developmental and Comparative Immunology. 25: 827-839.
Fast, M.D., Sims, D.E., Burka, J.F., Mustafa, A., and Ross, N.W. 2002. Skin
morphology and humoral non-specific defense parameters of mucus and
plasma in rainbow trout, coho and Atlantic salmon. Comp. Biochemical.
Physiology. A 132: 645-657.
Guardiola, F.A., Dioguardi, M., Parisi, M.G., Trapani, M.R., Meseguer, J., Cuesta,
A., and Esteban, M.A. 2015. Evaluation of waterborne exposure to heavy
metals in innate immune defenses present on skin mucus of gilthead seabream
(Sparus aurata), Fish and shellfish immunology, 45: 112-123.
Hajimoradloo, A., Ghorbani, R., and Abolfathi, M. 2014. Camparatie study on skin
mucus enzyme activity (lysozyme, trypsin, alkaline phosphatase) in four
aquarium spices. Gorgan University of agriculture sciences and natural
resources. 50p.
Harris, J.E., Harris and Hunt, S., Hunt, “The fine structure of iridophores inthe
skin of the Atlantic salmon (Salmo salar L.),” Tissue and Cell, 5(3): 479–488,
1973.
Hellio, C., Pons, A.M., Beaupoil, C., Bourgougnon, N., and Gal, Y.L. 2002.
Antibacterial, antifungal and cytotoxic activities of extracts from fish epidermis
and epidermal mucus. International journal of antimicrobial agents. 20: 214-
219.
Hordvik, I., Thevarajan, I., Thevarajan, J., Samdal, I., Samdal, Bastani, N., Bastani,
and B., Krossøy. 1999. “Molecular cloning and phylogenetic analysis of the
Atlantic salmon immunoglobulin D gene,” Scandinavian Journal of
Immunology, 50(2): 202–210.
Iger, Y., and Abraham, M. 1990. The process of skin healing in experimentally
wounded carp, Fish Biology. 36: 421–437.
Ingram, G.A. 1980. Substances involved in the natural resistance of fish to
infection–a review. Fish Biology, 16: 23-60.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein
measurement with the Folin phenol reagent. J. biol Chem, 193(1): 265-275.
Pigman, W., Pigman, “Mucus glycoprotein,” in The Glycoconjugates, Horowitz,
M.I., Horowitz and Pigman, W., Pigman, Eds., 1: 131–137, Academic Press,
New York, NY, USA, 1977.
Ross, N.W., Firth, Ross, K.J., Firth, Wang, A., Burka, Wang, J.F., Burka, and
Johnson, S.C., Johnson. 2000. “Changes in hydrolytic enzyme activities of
naive Atlantic salmon Salmo salar skin mucus due to infection with the salmon
louse Lepeophtheirus salmonis and cortisol implantation,” Diseases o Aquatic
Organisms, vol. 41(1): 1, 43–51.
Safari, R., Hajimoradloo, A., Jafarnodeh, A., Mohammadian, S., Neghadmoghaddam,
Sh. 2016. Collection of aquatic bacteria produced with genetic confirmation (the
first phase, Aeromonas hydrophila and Yersinia ruckeri). Research projects,
Gorgan University of agriculture sciences and natural resources. 42p.
Sancholi, O. 2012. Assessment of antibacterial properties of skin mucus of skin
mucus in deferent weight of common crap (Cyprinus carpio). Master of
sciences thesis, Gorgan University of agriculture sciences and natural resources.
74p.
Shepherd, K.L. 1994. Functions for fish mucus. Reviews in fish biology and
fisheries. 4: 401-429.
Shugar, D. (1952). The measurement of lysozyme activity and the ultra-violet
inactivation of lysozyme. Biochimica et biophysica acta, 8: 302-309.
Siwicki, A.K., and Anderson, D.P. 1993. Nonspecific defense mechanisms assay in
fish: II. Potential killing activity of neutrophils and macrophages, lysozyme
activity in serum and organs and total immunoglobulin level in serum. Fish
Disease Diagnosis and Prevention Methods Olsztyn, Poland. 1993: 105-12.
Subramanian, S., MacKinnon, S.L., and Ross, N.W. 2007. A comparative study on
innate immune parameters in the epidermal mucus of various fish species.
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular
Biology, 148(3): 256-263.
Suzuki, Y., Tasumi, S., Tsutsui, Sh., Okamoto, M., and Suetake, H. 2003.
Molecular diversity of skin mucus lectins in fish. Comprative Biochemistry and
Physiology Part B 136: 723-730.
Tabak, L.A. 1995. In defense of the oral cavity: structure biosynthesis and function
of salivary mucins. Annual Review Physiology. 57: 547-564.
Yano, T. 1996. The non-specific immune system: humoral defense. In: The fish
Immune system: Organism, Pathogen and Environment. Iwama, G., and
Nakanishi, T. (Eds). Academic press, San Diego, Pp: 105-157.