بهینه سازی استخراج ترکیبات ضداکسیدانی جلبک قهوه ای Sargassum angustifoliumبا رفلاکس حرارتی در روش تاگوچی

نویسندگان

1 دانشگاه گیلان

2 تربیت مدرس

چکیده

در این مطالعه از روش رفلاکس حرارتی برای استخراج عصاره از جلبک قهوه ای سارگاسوم استفاده شد. استخراج با 3 حلال (اتانول، آب/اتانول و آب) و در 3 زمان (90، 180 و 270 دقیقه) انجام شد. آزمایش‌ها با استفاده از طرح تاگوچی در سه سطح انجام شد. میزان فنول کل و خنثی کنندگی رادیکال آزاد سنجیده شد. با توجه به نتایج، بهترین شرایط برای استخراج ترکیبات ضداکسیدانی از سارگاسوم استخراج آبی و زمان 270 دقیقه بود. میزان فنول کل و IC50 در شرایط بهینه 392 میلی گرم اسید گالیک بر گرم ماده خشک (GAE) و 008/0 میلی گرم عصاره بود. نتایج نشان داد که این جلبک برای استخراج ترکیبات ضداکسیدانی مناسب می باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Optimization of antioxidant compounds extraction from brown algae Sargassum angustifolium in heating reflux methods using Taguchi design

نویسنده [English]

  • Aria Babakhani Lashkan 1

چکیده [English]

Reflux extraction was applied for extraction of algal extracts from brown algal species (Sargassum angustifolium). Extraction condition consists of three solvents (ethanol, ethanol/water; 50:50 and water), three extraction times (90, 180, 270min). All the experimental parameters were applied at three levels according to Taguchi's statistical design. Total phenol contents, DPPH scavenging activity of the extracts were determined. According to results, the best condition for extraction of antioxidant compounds from S.angustifolium was aqueous extract, and 270 minute extraction time. The TPC an IC50 in optimized treatment are respectively; 392 GAE (mg/gr dry matter) and 0.008 mg. Results show that this alga was suitable for the extraction of antioxidant compounds.

کلیدواژه‌ها [English]

  • Sargassum
  • natural antioxidant
  • Persian Gulf
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