Evaluation of the replacement laurel (Laurus nobilis) and Lawsonia Inermis L alcoholic essential oils with malachite green on the control Saprolegniasis in rainbow trout (Oncorhynchus mykiss) eggs

Document Type : scientific research article

Authors

1 Gorgan University of Agricultural Sciences and Natural Resources

2 1- Department Aquaculture, Faculty of Fisheries and Environment Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Department Fisheries and aquatic ecology, Faculty of Fisheries and Environment Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

4 Inland Waters Aquatic Stocks Research Center- Gorgan

Abstract

Background and objectives: This study was designed to prevent Saprolegniasis (Saprolegnia parasitica) in rainbow trout (Oncorhynchus mykiss) eggs using laurel (Laurus nobilis) and Lawsonia Inermis L alcoholic essential oils. This research was completely practical and carried out in environmental conditions.
Materials and methods: In this research, 3 treatments, each containing 3 concentrations, (9 treatments in total) were selected, and three trays with essential oils and two trays as controls with green malachite and pure water with temperature conditions of 13±2 degrees Celsius with a pH of 6.5-7.5 and a dissolved oxygen level of 6 ± 1 mg/liter for 18 days essential oils treatments are included 25, 50 and 70 mg/L and green malachite treatment is 3, 5 and 10 mg/L each with 3 repetitions by washing daily for 1 hour.
Results: The results showed that the combined use of two essential oils of with dose of 70 mg/liter had 70% survival in the egg emergence stage and based on statistical data, it had a significant difference with the samples and doses of the control treatments.
Conclusion: It can be concluded that the combined essential oils are suitable alternative to chemical disinfectants such as green malachite.

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