Document Type : scientific research article
Authors
1
Inland Water Aquaculture Research Centre, Iranian Fisheries Science Research Institute, Agriculture Research Education and Extension Organization, Bandar-e Anzali, Iran
2
University of GuilanFisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran
3
Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
Abstract
The present study was conducted to investigate spermatological and physiological parameters of semen of the Caspian roach (Rutilus caspicus) and their relationship in the Anzali lagoon migratory population during the breeding season (spring). Breeders were captured from the western part of the Anzali Lagoon and were transported to the hatchery at the Shahid Ansari Teleost Fish Restocking and Genetic Conservation Center (Rasht, Guilan, Iran). In order to perform the experiment, breeders received no hormonal induction. According to observations, the mean values of semen volume, sperm motility, motility time, spermatocrit, sperm concentration, and pH were measured as 0.4 mL, 96.1%, 54.4 s, 50.4%, 1.5 ×109/mL, and 7.9, respectively. Based on the evaluations performed on the seminal plasma, mean values of glucose, cholesterol, and triglycerides were measured as 4.8, 13.1, and 3.6 mg/dL, and mean values of total protein, albumin, and globulin were measured as 0.9 and 0.6, and 0.3 g/dL, respectively. The concentrations of sodium, potassium, calcium, and magnesium ions were also measured as 161.9, 47.3, 5.5, and 3.8 mM/L respectively. The results revealed a significant positive correlation between spermatocrit value and spermatozoa concentration (P < 0.01). In addition, there was a significant positive correlation between the concentration of total protein, globulin, and albumin (P < 0.01), and a significant negative correlation was observed between seminal plasma cholesterol and potassium concentrations (P < 0.05). Moreover, a significant positive correlation was obtained between albumin and globulin concentrations (P < 0.05). No significant correlation was observed among the other measured parameters. The results of the present study could help to improve the controlled reproduction process and the preparation of suitable solutions for Caspian roach semen storage.
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