Effect of sub lethal doses of silver nitrate (Ag No3) on the expression of antioxidant genes (CAT and SOD), stress related genes (HSP70) and liver enzymes of zebrafish (Danio rerio)

Document Type : scientific research article

Authors

1 Gorgan university

2 gorgan university

3 Associate Prof

10.22069/japu.2023.21668.1810

Abstract

The purpose of this research was to investigate the effect of sub-lethal concentrations of silver nitrate (AgNO3) on antioxidant and stress genes expression and some biochemical indices of zebra fish. For this purpose, zebra fish with the average weight of 0/5±0/03 g were exposed to different levels of silver nitrate (T1: 10%, T2: 25% and T3: 50%) and control group for 14 days, each containing 3 replicates. At the end of the experiment, samples were collected to evaluate Sod, Cat, HSP70 and IGF-I genes expression and some biochemical factors including: ALP, ALT and AST. Results showed that genes expression (SOD, CAT and HSP70) were up-regulated at 25 and 50% which had significant differences with control (P<0/05). The highest IGF-I level significantly was observed in control and the lowest expression was in 50% treatment (P<0/05). ALP, ALT and AST activities in fish exposed to 10% silver nitrate had remarkable differences with others groups (P<0/05). Therefore, it can be concluded, silver nitrate had destructive effects on zebra fish which can be used to evaluate the effect of heavy metals on fish.

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Journal of Utilization and Cultivation of Aquatics
Volume 14, Issue 1
April 2025
Pages 221-233

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