Investigation on zebrafish (Danio rerio) physiological responses exposed to Butachlor herbicide

Document Type : scientific research article

Authors

1 Corresponding Author, Ph.D. Student of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Dept. of Aquatic Ecology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

The aim of this study was to evaluate the effect of sub-lethal concentrations of Butachlor on some antioxidant responses and stress genes expression and some biochemical indices of zebra fish. After determination of LC50, for 96 hours, zebra fish (0/3±0/01 g) in four experimental groups including (T1: 10%, T2: 25%, T3: 50% of LC50 and control) were exposed to different levels of the herbicide Bbutachlor for 14 days. At the end of the exposure to Butachlor, samples of whole body were collected to measured SOD, CAT and HSP70 genes expression and some biochemical indices including: ALP, ALT and AST. At the end of experimental period, results revealed that SOD, CAT and HSP70 genes expression were up-regulated at 25 and 50% treatments that had significant differences with 10% and control (P<0/05). ALP, ALT and AST activities in fish treated with 25% Butachlor had remarkable differences with others groups (P<0/05). Therefore, it can be concluded theat, Butachlor had destructive effects on zebra fish.

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References

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Journal of Utilization and Cultivation of Aquatics
Volume 14, Issue 2
July 2025
Pages 57-67

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