Extraction and purification of lipase (EC 3.1.1.3) from the Skipjack intestine (Katsuwenus pelamis)

Document Type : scientific research article

Authors

1 Corresponding Author, Persian Gulf and Sea of Oman Ecology Research Center, Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Bandar Abbas, Iran

2 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

About 7 to 8% of the body weight of tuna fish is the digestive tract and it is an economic source for lipase enzyme extraction. Enzyme extraction was done using Tris hydrochloride buffer (25 mM with pH; 7.8). Then the crude extract was precipitated and dialyzed in the range of saturation between 30, 45 and 60% ammonium sulfate. For further purification, ion exchange chromatography using a DEAE-CMC column with dimensions (2.5 × 30 cm) and gel filtration chromatography using a Sephadex G-100 column (1.8 × 80 cm) were used. Purified lipase enzymes obtained by precipitation with ammonium sulfate 30, 45 and 60%, respectively, have molecular weight (68.3, 38.41, and ND KDa), pH (7.1, 6.9, and 7.4), The optimal temperature (55, 42 and 38 °C) and specific enzyme activity (3.42, 2.8 and 1.4 U/mg) were using paranitrophenyl palmitate (pNPP) as a substrate. The results indicate the possibility of extracting and purifying lipase enzyme from the digestive system of aquatic animals with suitable biochemical characteristics for use in chemical and food industries.

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References

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Journal of Utilization and Cultivation of Aquatics
Volume 13, Issue 3
December 2024
Pages 115-128

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