Induction of antioxidant and immune genes expression in zebra fish (Danio rerio) exposed to polystyrene nanoplastic

Document Type : scientific research article

Authors

1 Dept. of Aquatics Production and Exploitation, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Corresponding Author, Dept. of Aquatics Production and Exploitation, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

3 Dept. of Aquatics Production and Exploitation, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

4 Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

During recent years, nanoplastics have been widely entered into aquatic environments so that nanoplastics pollution has become a global concern. In this regard, information on nanoplastics toxicity is limit for freshwater aquatics, especially at the molecular level. Thus, in the present study, effect of polystyrene nanoplastic was investigated on antioxidant and immune genes expression in zebra fish (Danio rerio). For this, polystyrene neoplastic was synthetized and sprayed on fish food. Zebra fish were then fed with the food containing 1, 2, 4 and 8% polystyrene nanoplastic, respectively, for 14 days. One group was also fed with the fish food without nanoplastic as the control. The relative gene expression levels of SOD, CAT and HSP70 were assessed after 14 days. The results indicated that polystyrene nanoplastic induced antioxidant genes expression so that a considerable increase was observed in CAT and SOD genes levels. An increase trend was also observed in antioxidant genes levels with the increase in polystyrene concentration up to 8%. Polystyrene also induced the HSP70 gene. The observed trend in HSP70 gene expression level with the increase in nanoplastic concentration was similar to the antioxidant genes. Considering the results, it could be said that nanoplastic can induce immune and antioxidant system in zebra fish.

Keywords

Main Subjects

References

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Journal of Utilization and Cultivation of Aquatics
Volume 13, Issue 3
December 2024
Pages 1-12

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