Investigation of some innate immune indicators, liver metabolic enzymes, growth and survival in zebrafish (Danio rerio) fed with different levels of autolysed yeast based on Saccharomyces cerevisiae

Document Type : scientific research article

Authors

1 Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Corresponding Author, Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

The present research was conducted with the aim of determining the effects of diet containing autolysed Saccharomyces cerevisiae yeast on innate immune function, liver metabolic enzymes, growth and survival of zebrafish. For this purpose, 480 pieces of zebra fish were randomly placed in aquariums with a water volume of 40 liters for 60 days with different levels of autolyzed yeast in the basic diet including 1, 2 and 5% of the diet along with a control group (three replications) to 5% of body weight were fed. At the end of the course, to check survival and growth performance (final weight, percentage of body weight gain, food conversion ratio, specific growth rate, obesity ratio), biometry was done on all fish. In order to measure some of the serum's inherent immunity indicators, such as total immunoglobulin, total protein, albumin, and to check liver metabolic enzymes (alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase), fish were randomly sampled in order to prepare serum. 5 pieces of each aquarium). Data were analyzed using SPSS software through one-way analysis of variance. The results showed that feeding zebrafish with different levels of yeast isolates had no significant effect on the growth performance, survival percentage and liver enzymes of the treatment groups compared to the control group (p<0.05). However, the highest amount of total protein, total immunoglobulin and serum albumin was significantly in the treatment of feeding with autolysed yeast at the rate of 5% of the diet (p<0.05). In general, the results showed that the use of synthesized yeast, despite its effect on growth, liver enzymes and survival, could improve the innate immunity indicators in zebrafish. And the best suggested level in this research is 5% of autolysed yeast diet.

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Main Subjects


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