Effect of dietary administration of lactobacillus plantarum on reproduction related genes expression Vitellogenin and Estrogen receptor and cyp19a) Sex hormones ( progesterone, estradiol) and histology of gonad in female of zebrafish (Danio rerio) on Sex hormone estradiol and histology of gonad in female of zebrafish (Danio rerio)

Document Type : scientific research article

Authors

1 Ph.D. Student, Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Corresponding Author, Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

4 . Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

The objective of this study was to investigate the effect of different levels of Lactobacillus plantarum probiotic on estradiol hormone and gonad tissue in zebrafish (Danio rerio). So, six hundred healthy zebrafish (0.15+0.01 gr) were randomly distributed in 12 glass aquariums and fed experimental diets contain different levels of L. plantarum (0%, 105, 106 and 107CFU/gr ) for 3 months . After this period and sex determination, males were seprated and females fed probiotic for 1 month more. At the end of the experiment, the whole body of the fish was used to measure the hormone, the amount of esteroid hormone was measured by RIA method. Also of the histological point of view were studied by light microscope. The results of this study showed an increase in the amount of estradiol hormone during vitellogenesis in fish fed 106 CFU/gr L. plantarum which had a significant difference with other treatments (P <0.05). However, 107 CFU/g fed L. plantarum treatment had a decreasing trend. In tissue sections, the most of the eggs in control were clearly in the second and third stages of sexual maturation while in the 106 CFU/gr fed treatment, most of the eggs were in the third and fourth stages and even at the end of the fourth stage of maturation.These stages were more common than treatments fed 105 CFU/g and 107 CFU/g L. plantarum. The results of the present study indicated that the use of probiotics L. pentarum in the effective amounts can be considered as a new strategy to improve reproductive performance.

Keywords

References

1.FAO. 2018. The State of World Fisheries and Aquaculture 2018 - Meeting the Sustainable Development Goals, Food and Agriculture Organization of the United Nations, Rome, Italy.
2.FAO. 2020. Sustainability in action, state of world fisheries and aquaculture 2020. Rome.
3.El-Saadony, M.T., Alagawany, M., Patra, A.K., Kar, I., Tiwari, R., Dawood, M.A., and Abdel-Latif, H.M. 2021. The functionality of probiotics in aquaculture: an overview. Fish Shellfish Immunol. 117: 36-52.
4.Oryan, S.H., Parivar, K., Yekrangeyian, A., and Hossienzade, H. 1997. Seasonal cycles variation of gonadal development and plasma sex steroids levels in Trichiurus lepturus. Iran Sci. Fish. J.7: 49-67.
5.Poortenaar, C.W., Hooker, S.H., and Sharp, N. 2001. Assessment of yellowtail king Fish (seriola lalandi lalandi) reproductive physiology, as a basis for aquaculture development. Aquac. Res. 201: 271-286.
6.Dawood, M.A., El-Dakar, A., Mohsen, M., Abdelraouf, E., Koshio, S., Ishikawa, M., and Yokoyama, S. 2014. Effects of using exogenous digestive enzymes or natural enhancer mixture on growth, feed utilization, and body composition of rabbitfish, Siganus rivulatus. J. Agric. Sci. Technol. 4(3B).
7.Lauriano, E.R., Pergolizzi, S., Capillo, G., Kuciel, M., Alesci, A., and Faggio, C. 2016. Immunohistochemical characterization of Toll-like receptor 2 in gut epithelial cells and macrophages of goldfish Carassius auratus fed with a high-cholesterol diet. Fish Shellfish Immunol. 59: 250-255.
8.Aragona, M., Lauriano, E.R., Pergolizzi, S., and Faggio, C.J.N.P.R. 2018.Opuntia ficus-indica (L.) Miller as a source of bioactivity compounds for health and nutrition. Nat. Prod. Res.32: 17. 2037-2049.
9.Nath, S., Matozzo, V., Bhandari, D., and Faggio, C. 2019. Growth and liver histology of Channa punctatus exposed to a common biofertilizer. Nat. Prod. Res. 33: 11. 1591-1598.
10.Irianto, A., and Austin, B. 2002. Probiotics in aquaculture. J. Fish Dis. 25: 11. 633-642.
11.Dawood, M.A., and Koshio, S. 2016. Recent advances in the role of probiotics and prebiotics in carp aquaculture: a review. Aquac. Res. 454: 243-251.
12.Ribas, L., and Piferrer, F. 2014. The zebrafish (Danio rerio) as a model organism, with emphasis on applications for finfish aquaculture research. Rev. Aquac. 6: 4. 209-240.
13.Shahsavani, D., Mohri, M., and Gholipour Kanani, H. 2010. Determination of normal values of some blood serum enzymes in Acipenser stellatus Pallas. Fish Physiol. Biochem. 36: 1. 39-43.
14.Evans, D.H., Claiborne, J.B., and Currie, S. eds. 2013. Fish Physiol. CRC press.
15.Figueiredo-Fernandes, A., Ferreira-Cardoso, J.V., Garcia-Santos, S., Monteiro, S.M., Carrola, J., Matos, P., and Fontaínhas-Fernandes, A. 2007. Histopathological changes in liver
and gill epithelium of Nile tilapia, Oreochromis niloticus, exposed to waterborne copper. Pesqui. Vet. Bras. 27: 3.103-109.
16.Fanta, E., Rios, F.S.A., Romão, S., Vianna, A.C.C., and Freiberger, S. 2003. Histopathology of the fish Corydoras paleatus contaminated with sublethal levels of organophosphorus in water and food. Ecotoxicol. Environ. Saf. 54: 2. 119-130.
17.Carnevali, O., Maradonna, F., and Gioacchini, G. 2017. Integrated control of fish metabolism, wellbeing and reproduction: the role of probiotic. Aquac. Res. 472: 144-155.
18.Matty, A.L. 1985. Fish Endocrinology, croom Helm london, 160p.
19.Lee, W.K., and Yang, S.W. 2002. Relationship between ovarian development and serum levels of gonadal steroid hormones, and induction of oocyte maturation and ovulation in the cultured female Korean spotted sea bass Lateolabrax maculatus (Jeom- nong-eo). Aquac. Res. 207: 1-2. 169-183.
20.Habibi, H.R. 2002. Fish Reproduction Physiology Workshop, Rasht.
21.Zhou, Y., Li, S., Pang, Q., and Miao,Z. 2020. Bacillus amyloliquefaciens BLCC1-0238 can effectively improve laying performance and egg quality via enhancing immunity and regulating reproductive hormones of laying hens. Probiotics Antimicrob. 12: 1. 246-52.
22.Zhao, S., Zhang, K., Ding, X., Celi, P., Yan, L., and Bai, S. 2019. The impact of dietary supplementation of different feed additives on performances of broiler breeders characterized by different egg-laying rate. Poult. Sci. 98: 11. 6091-9.
23.Carnevali, L., Krug, B., Amant, F.,Van Pee, D., Gérard, V., De Béthune, X., and Spinewine, A. 2013. Performance of the adverse drug event trigger tool and the global trigger tool for identifying adverse drug events: experience in a Belgian hospital. Ann. Pharmacother. 47: 11. 1414-1419.
24.Watanabe, T., and Kiron, V. 1995. Broodstock management and nutritional approaches for quality offsprings
in the Red Sea Bream. Broodstock management and egg and larval quality. pp. 154-195.
25.Bromage, N.R. 1995. Broodstock management and seed quality-general considerations. Brood-stock management and egg and larval quality. pp. 1-24.
26.Gioacchini, G., Maradonna, F., Lombardo, F., Bizzaro, D., Olivotto, I., and Carnevali, O. 2010a. Increase of fecundity by probiotic administration in zebrafish (Danio rerio). Reprod.140: 6. 953-959.
27.Gioacchini, G., Bizzaro, D., Giorgini, E., Ferraris, P., Sabbatini, S., and Carnevali, O. 2010b. Oocytes maturation induction by Lactobacillus rhamnosus in Danio rerio: in vivo and in vitro studies. Hum. Reprod. 25: I205-I206.
28.Gioacchini, G., Giorgini, E., Ferraris, P., Tosi, G., Bizzarro, D., Silvi, S., and Carnevali, O. 2010c. Could probiotics improve fecundity Danio rerio as case of study. J. Biotechnol. 150: 59-60.
29.Gioacchini, G., Lombardo, F., Merrifield, D.L., Silvi, S., Cresci, A., Avella, M.A., and Carnevali, O. 2011. Effects of probiotic on zebrafish reproduction. J. Aquac. Res. Dev. S. 1.
30.Sadani, A., Safari, R., Imanpour, M.R., and Jafar Nodeh, A. 2018. Effect of dietary administration of Lactobacillus casei on reproduction related genes expression (Vitellogenin and Estrogen Receptor) in female zebrafish (Danio rerio). Gorgan University of Agricultural Science and Natural Resources.
31.Rahman, M.L., Akhter, S., Mallik, M.K.M., and Rashid, I. 2018. Probiotic enrich dietary effect on the reproduction of butter catfish, Ompok pabda (Hamilton, 1872). Int. J. Curr. Res. 7: 2. 866-873.
Journal of Utilization and Cultivation of Aquatics
Volume 11, Issue 4
February 2023
Pages 33-42

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