Extraction of phycocyanin from spirulina microalgae and evaluation of the stability of nanoliposomes incorporated with pigment against environmental conditions

Document Type : scientific research article

Authors

1 Ph.D. Student of Sea Food Processing, Faculty of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Corresponding Author, Associate Prof., Dept. of Sea Food Processing, Faculty of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

3 Associate Prof., Dept. of Sea Food Processing, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

4 Ph.D. Graduate of Sea Food Processing, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

The encapsulation technique in lipid nanocarriers can be an effective way to resolve the limitations in the utilization of phycocyanin pigment extracted from spirulina due to special taste and odor, high sensitivity of these compounds, and undesirable color in products. Therefore, in the present study, phycocyanin was extracted from Spirulina platensis and its concentration was investigated. Nanoliposomes with chitosan coating (0 and 1% w/v) containing pigment were prepared and its physicochemical properties, morphology, and stability to different environmental conditions (relative humidity, temperature, and light) were evaluated. The mean size of nanoliposomes and polydispersity index ranged from 322.21 to 426.31 nm and from 0.27 to 0.28 in nanocarriers, respectively. The highest values of encapsulation efficiency of nanoliposome containing phycocyanin (81.4%) were obtained under optimal conditions in nanoliposomes with chitosan coating. Evaluation of the stability of liposomes against light, relative humidity, and different temperatures over storage showed an increase in the stability of phycocyanin encapsulated in lipid carriers and chitosan as a liposome coating increased the stability and controlled release of phycocyanin.

Keywords

References

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Journal of Utilization and Cultivation of Aquatics
Volume 11, Issue 1
June 2022
Pages 18-30

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