The effects of different levels of raffinoz on growth index and antioxidant defense in common carp fingerling(Cyprinus carpio)

Document Type : scientific research article

Authors

1 gorgan university of agricultural and sciences and natural resources

2 Gorgan University of agricultural sciences and natural resources

3 Gorgan university of agricultural sciences and natural resources

Abstract

Abstract:
In this research, the effect of peribiotic supplementation of raffinose on the growth performance of Mucus immuno component in Cyprinus carpio was investigated. In this experiment, carps (weighted average 10 grams) were fed diets containing various levels of raffinose peribiotic including zero (control), 1, 2 and 4 g / kg for 8 weeks. Each treatment was submerged in 3 replicates. At the end of the experiment, mucus sampling and growth parameters were measured. The results of the study showed that total mucus immunoglobulin content in treated fish with raffinose was significantly higher than control group (P <0.05) and the highest amount was recorded in 4 g treatments. Lysozyme activity increased in probiotic treatments compared with control group, however, there was no significant difference (P <0.05). In addition, growth parameters (final weight, weight gain, specific growth factor), Food conversion ratio and survival percentage were not significantly different in the treated groups compared to the control group (P <0.05).

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  1. Akrami, A., Karim Abadi, A., Mohammadzade, H., and Ahmadifar, A. 2009. The Effect of prebiotic mannan oligosaccharide on Growth, Survival, body Composition and resistance to salinity stress in whitefish (Rutilus frisii kutum). Journal of Marine Science and Technology. 47-57
  2. Akrami, A., Zarei, A., Ghlichi, A. 2011. Influence of inulin Supplementation as a prebiotic on Growth, Survival, Intestinal Lactic acid bacteria density,and carcass composition of common carp(cyprinus carpio). Fisheries Magazine, Islamic Azad University, Azadshahr Branch. 1-16.
  3. Akrami, A., Ghlichi, A., Ebrahimi, A. 2008. The Effect of Different Levels of Inhibitory prebiotic on the Growth and Survival of Rainbow Trout (Oncorhynchus mykiss). First National Conference on The Science of Fisheries and Aquaculture in Iran.Islamic Azad University, Lahijan Unit. 10-12.
  4. Al‐Banaw, A., Kenngott, R., Al‐Hassan, J., Mehana, N., and Sinowatz, F. 2010. Histochemical analysis of glycoconjugates in the skin of a catfish (Arius Tenuispinis, Day). Anatomia, Histologia, Embryologia., 39: 42-50.
  5. Aly, S.M., Atti, N.M.A., and Mohamed, M.F. 2008. Effect of garlic on the survival,     growth, resistance and quality of Oreochromis niloticus. In 8th International Symposium on Tilapia in Aquaculture.
  6. Fric, P. 2007. Probiotics and prebioticsrenaissance of a therapeutic principle. Central European Journal of Medicine., 2(3): 237-270.
  7. Gatlin, III, D.M. 2002. Nutrition and fish health. Fish nutrition. 3: 671-702.
  8. Gibson, G.R., and Roberfroid, M.B. 1995. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. Journal of Nutrition. 125: 1401-1412.
  9. Hajibeglou, A., and Sudagar, M. 2011. Effect of Dietary Probiotic Level on the Reproductive Performance of Female Platy (Xiphophorus maculatus). Agricultural Journal. 6: 119-123.
  10. Hevrøy, E., Espe, M., Waagbø, R., Sandnes, K., Ruud, M., and HEMRE, G.I. 2005. Nutrient utilization in Atlantic salmon (Salmo salar L.) fed increased levels of fish protein hydrolysate during a period of fast growth. Aquaculture Nutrition., 11: 301-313.
  11. Hong-Yun, G., Lan-Mei, W., Yin-Hua, ZH., Min, X., Wei-Xiang, ZH., Xiu-Feng, W., Liang, H., Jia, W. 2010. Effects of raffinose on growth performance, immunity, stress/response and survival of Japanese seabass (lateolabrax/japonicus) challenged with aeromonas hydrophila. CNKI JOURNAL.
  12. Hosseinifar, S.H., and Mahious, A.S. 2007. Probiotics, prebiotics and synbiotics in aquaculture: A review. Proceeding of the First International Training Course on fish Nutrition and disease. Ghaemshahr, Iran.
  13. Hoseinifar, S.H., Mirvaghefi, A., Mojazi Amiri, B., and Merrifield, D.L. 2011. The effects of oligofructose on growth performance, survival, intestinal microbiota and liver histoligy of of endangered great sturgeon (Huso huso) juvenile. Aquaculture Nutrition. 17: 497-504.
  14. Hoseinifar, S.H., Soleimani, N., Ringo, E. 2014. Effects of dietary fructo-oligosaccharide supplementation on the growth performance,hemato-immunological parameters,gut microbiota and stress resistance of common carp (cyprinus carpio) fry, Br y nutr.
  15. Kiron, V. 2012. Fish immue system and its nutritional modulation for preventive health care, Animal feed Sci. Technol., 33: 111-173.
  16. Lazado, C.E., and Caiping, C.M.A. 2014. Bacterial viability differentially influences the immunomodulatory capabilities of potential host-derived probiotics in the intestinal epithelial cells of Atlantic cod (Gadus morhua), J APP microbial. 116: 990-8.
  17. Kolangi Miandare, H., Farvardin, Sh., Shabani, A., Hoseinifar, S.H., Ramezanpour, S. 2016. The effects of galactooligosacharide on systemic and mucosal immune respone, growth performance and appetite related gene transcript in goldfish(Carassius auratus gibelio). Fish and shellfish immunology. 55: 479-483.
  18. Ringo, E., Dimitroglou, A., Hoseinifar, S.H., Davies, S.J. 2014. Probiotics in finfish; an update.In Ringo, E, merrifield, D, edirors. Aquaculture nutrition: gut health probiotics and prebiotics.1 ed.wiley-Blackwell.p. 416.
  19. Ross, N.W., Firth, K.J., Wang, A., Burka, J.F., and Jojnson, S.C. 2000. Changes in hydrolytic enzyme activities of Atlantic salmon (Salmo salar) skin mucus due to infection with the salmon louse (Lepeophtheirus salmonis) and cortisol implantation. Disease of Aquatic Organisems. 41: 43-51.
  20. Sheikheslami Amiri, M., Yousefain, M., Yavari, A., Mohammadian, T., Abhari, H., Guran, H. 2009. "Stimulation of Rainbow trout (Oncorhynchus mykiss) and increasing resistance to streptococcus by supplementing the prebiotic inulin to diet. "Summary of articles of the first National Conference on Caspian Sea Fishery Resources, 68p.
  21. Siwicki, A.K., and Anderson, D.P. 1993. Nonspecific defense mechanisms assay in fish: II. Potential killing activity of neutrophils and macrophages, lysozyme activity in serum and organs and total immunoglobulin level in serum. Fish Disease Diagnosis and Prevention Methods Olsztyn, Poland. 1993: 105-12.
  22. Soleimani, N., Hoseinifar, S.H., Merrifield, D.L., Barati, M., and Abadi, Z.H. 2013. Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish and Shellfish Immunology. 32: 316-321.
  23. Subramanian, S., MacKinnon, Sh.L., and Ross, N.W. 2007. A comparative study on innate immune parameters in the epidermal mucus of various fish species. Comprative Biochemistry and Physiology, Part B. 148: 256-263.
  24. Subramanian, S., Ross, N.W., MacKinnon, S.L. 2008. Comparison of antimicrobial activity in the epidermal mucus extracts of fish. Comparative Biochemistry and Physiology. 150: 85-92.
  25. Sudagar, M., and Hoseinifar, S.H. 2005. The use of Optimun in diet of grand sturgeon (Huso huso) fry and its effects on growth factors and survival rate.
  26. Proceedings of the 5th international symposium on sturgeons, Ramsar, Iran. 9-13 may, 93p. 
  27. Tacon, A. 1990. Standards methods for the nutrition and feeding of farmed fish and shrimp.
  28. Whyte, SK. 2007. The innate immune response of finfish e a review of current knowledge. Fish Shellfish Immuno. 23: 1127-51.