Comparative study of red blood cell abnormalities in the Caspian trout (Salmo trutta caspius), rainbow trout (Oncorhynchus mykiss) and their triploid hybrids

Document Type : scientific research article

Authors

1 Department of Natural Resources (Fisheries Division), Isfahan University of Technology, Isfahan, Iran

2 Isfahan University of technology, Natural Resource College (Fisheries Division), Isfahan, Iran

Abstract

Since the shape and size of red blood cells are very impotant in physiological aspects of animals siences, specially in respirations and nutrient transportation, this study was conducted to survay the changes in shape and size of red blood cell (abnormalities) in rainbow trout (Oncorhynchus mykiss), the Caspian trout (Salmo trutta caspius) and their triploid hybrids (Oncorhynchus mykiss ♀× Salmo trutta caspius ♂). Triploidy was induced in eggs using 10-min heat shock at 28ºC following 10-min post fertilization. The accuracy of ploidy induction was determined by comparison the cell size. The results from comparison of rainbow trout, caspian trout and their triploid hybrids showed that in all aspects of red blood cells such as large and small axes of nucleus and cell, axis of nucleus and cell, volume and area of nucleus except volume of cell, there is not significant differences between rainbow trout and caspian trout (p>0.05) but triploidy leads to a significant increase in all mentioned chrachters in triploid hybrids relative to caspian trout and rainbow trout (p < 0.05). The morphological abnormalities in the red triploid cells were noticeably higher than diploids. The most abundant types of abnormalities include poikilocytosis, macrocytosis, and incomplete cytoplasmic cells. Triploid hybrids have many alterations of hematological factors that these alterations may affect stress responses, immunology, health conditions, growth, and survival triploid.

Keywords

Main Subjects

References

  1. Akhan, S., Serezli, R., and Sonay, F.D. 2011. Hematology of Diploid and Triploid Rainbow Trout (Oncorhynchus mykiss), Black Sea Trout (Salmo labrax Pallas, 1814), and their F1 Hybrids. Isr. J. Aquacult-Bamid. 63(577): 1-7.
  2. Benfey T.J. 1999. The physiology and behavior of triploid fishes. Rev. Fish. Biol. Fish. 7: 39-67.
  3. Benfey, T.J., and Sutterlin, A.M. 1984. The haematology of triploid landlocked Atlantic salmon, Salmo salar, L.J. Fish Biol., 24: 333–338.
  4. Beyea, M.M., Benfey, T.J., and Kieffer, J.D. 2005. Haematology and stress physiology of juvenile diploid and triploid shortnose sturgeon (Acipenser brevirostrum). Fish. Physiol. Biochem., 31: 303–313.
  5. Cal, R.M., Vidal, S., Camacho, T., Piferrer, F., and Guitian, F.J. 2005. Effect of triploidy on turbot haematology. Comp. Biochem. Physiol. A. 141: 35–41.
  6. Cogswell, A.T., Benfey, T.J., and Sutterlin, A.M. 2002. The haematology of diploid and triploid ransgenic Atlantic salmon (Salmo salar). Fish. Physiol. Biochem., 24: 271–277.
  7. Dorafshan, S. 2007. Chromosomal manipulation of Caspian Salmon and Rainbow trout and Growth Comparison in F1 Generation. Ph.D Thesis. Tarbiat Modares University, Iran.
  8. Dorafshan, S., Kalbassi, M.R., Pourkazemi, M., Amiri, B.M., and Karimi, S.S. 2008. Effects of triploidy on the Caspian salmon Salmo trutta caspius haematology. Fish. Physiol. Biochem., 34: 195-200.
  9. Felip, A., Piferrer, F., Zanuy, S., and Carrillo, M. 2001. Comparative growth performance between diploid and triploid sea bass (Dicentrarchus labrax L.) over the first four spawning seasons. J. Fish. Biol., 58: 76– 88.
  10. Graham, M.S., Fletcher, G.L., and Benfey, T.J. 1985. Effect of triploidy on blood oxygen content of Atlantic salmon. Aquaculture. 50: 133–139.
  11. Kim, D.S., Jo, J.Y., and Lee, T.Y. 1994. Induction of triploidy in mud loach (Misgurnus mizolepis) and its effect on gonadal development and growth. Aquaculture., 120: 263–270.
  12. Lemoine, L.H., and Smith, T.L. 1980. Polyploidy induced in brook trout by cold shock. Tran. Am. Fish. Soc., 109: 626–631.
  13. Miller, B., Potter-Locher, F., Seelbach, A., Stoppper, H., Utesch, D., and Madle, S. 1998. Evaluation of the in vitro micronucleus test as an alternative to the in vitro chromosomal aberration assay: position of the GUM working group on the in vitro micronucleus test. Mutat. Res., 410: 81–116.
  14. O’Keefe, R., Stillwell, E., and Benfey, T.J. 2000. The effect of transportation and handling on the haematology of diploid and triploid Atlantic salmon (Salmo salar). Aquac. Assoc. Canada. Spec. Publ., 4: 25–27.
  15. Sadler, J., Wells, R.M.G., Pankhurst, P.M., and Pankhurst, N.W. 2000. Blood oxygen transport, rheology and haematological responses to confinement stress in diploid and triploid Atlantic salmon, Salmo salar. Aquaculture., 184: 349– 361.
  16. Scheerer, P.D., and Thorgaard, G.H. 1983. Increased survival in salmonid hybrids by induced triploidy. Can. J. Fish. Aquat. Sci., 40: 2040–2044.
  17. Sedgwick, S.D. 1995. Trout farming handbook, 5th edn. Fishing News Books, Alden Press, Oxford.
  18. Sezaki, K., Watabe, S., Tsukamoto, K., and Hashimoto, K. 1991. Effects of increase in ploidy status on respiratory function of ginbuna, Carassius auratus langsdorfi (Cyprinidae). Comp. Biochem. Physiol., 32: 123–127.
  19. Strunjak-Perovic, I., Coz-Rakovac, R., and Topic-Popovic, N. 2003. Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum). Vet. Med. Czech., 48: 215–219.
  20. Svobodova, Z., Kolarova, J., and Flajshans, M. 1998. The first finding of the differences in complete blood count between diploid and triploid tench, Tinca tinca L. Acta. Vet. Brno., 67: 243–248.
  21. Virtanen, E., Forsman, L., and Sundby, A. 1990. Triploidy decreases the aerobic swimming capacity of rainbow trout (Salmo garidneri). Comp. Biochem. Physiol., 96A: 117–121.
  22. Wattendorf, R.J. 1986. Rapid identification of triploid grass carp with a Coulter Counter and Channelizer. Prog. Fish. Cult., 48: 125– 132.
  23. Własow, T., Kuzminski, H., Woznicki, P., and Ziomek, E. 2004. Blood cell alteration in triploid brook trout Salvelinus fontinalis (Mitchill). Acta. Veterinaria. Brno., 73(1): 115-118.
  24. Yamamoto, A., and Iida, T. 1994. Haematological characteristics of triploid rainbow trout. Fish. Pathol., 29: 239–243.
  25. Yokote, M. 1982. Vascular system. In: Hibiya T. (ed.) an atlas of fish histology normal and pathological features, Gustav Fischer Verlag. Stuttgart. 58-73.
Journal of Utilization and Cultivation of Aquatics
Volume 8, Issue 1
May 2019
Pages 1-9

Files

Share

How to cite

Statistics