The study of in-vitro effect of some chemical factors on enzymes activity of trypsin and chymotrypsin in rainbow trout (Oncorhynchus mykiss) fry

Authors

1 Department of fisheries, Faculty of natural sciences and environmental, Malayer University, Malayer, Hamedan

2 M.Sc graduated of Fisheries, Marine Sciences Faculty, Tarbiat Modares University, Nour, Mazandaran, Iran

3 Fisheries Department, Faculty of Agriculture and Natural Resources, Persian Gulf University, Borazjan, Bushehr, Iran.

Abstract

In this study, the chemical factors effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors on the activity of trypsin and chymotrypsin of rainbow trout fry were considered. The results showed that K+ and Na+ had not significant decrease on trypsin and chymotrypsin activity than did those from control (P>0.05). Ca2+ and Mg2+ showed a significant increase in activity of trypsin and chymotrypsin (P0.05). Chymotrypsin activity showed a significant decrease in the presence of TPCK, Iodoacetic acid, EDTA and ß-Mercaptoethanol than did those from control (P

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