Effect of dietary administration of Pectin and Natuzyme multi-enzyme on growth performance, growth (GH and IGF-1) and immune (IL1-β and Lyz) related genes expression in common carp (Cyprinus carpio)

In this experiment, the effect of using pectin and natozyme multi-enzyme separately and in combination on blood serum biochemical indicators in common carp fry (Cyprinus carpio) was investigated. For this purpose, the number of 540 common carp fry with an average weight of about 10 gr for 8 weeks with experimental diets with nine treatments and each treatment with three repetitions including basic diet (0), 1% pectin, 2% pectin 0.05% natozyme multi-enzyme, 0.1% natozyme multi-enzyme, combination of pectin (1%) and natozyme multi-enzyme (0.05%), pectin combination (2%) and natozyme multi-enzyme (0.05%), Pectin composition (1%) and natozyme multi enzyme (0.1%), pectin composition (2%) and natozyme multi enzyme (0.1%) were fed. . For genetic study, liver, brain and kidney tissues were sampled; RNA extraction and cDNA synthesis were performed using kits. Growth-related genes (GH and IGF-1) and immunity (IL1-β and Lyz) were evaluated using real time PCR. The data were evaluated by two-way analysis of variance using SPSS software. In the results of the separate and combined effects of natozyme and pectin on the expression of growth genes, no significant difference was observed in the separate treatments of natozyme and pectin (P>0.05), but the combined treatment with an interaction effect showed a significant difference with other treatments (P≤0.05). The results of separate effects of natozyme and pectin on LYZ and IL-1 gene expression, there was a significant difference between the separate treatments of natozyme and pectin, so that pectin was higher than natozyme (P≤0.05). In addition, combined treatment with interaction effect showed a significant difference with other treatments (P≤0.05). The results of this experiment showed that the use of 0.1% natozyme multienzyme and 2% pectin in the diet of common carp can have a positive effect on the expression of growth and immunity genes in this fish.