Study on liver lesions and mucosal indices of common carp (Cyprinus carpio) in exposure to different concentrations of nanoplastic

Document Type : scientific research article

Authors

1 M.Sc. Student, Dept. of Fisheries and Aquatic Ecology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Corresponding Author, Associate Prof., Dept. of Fisheries and Aquatic Ecology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Associate Prof., Dept. of Fisheries, Faculty of Natural Resources and Marine Sciences, Khorramshahr University of Marine Sciences and Technology, Khorramshahr, Iran.

4 Ph.D. of Fisheries, Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

5 Ph.D. Student, Dept. of Fisheries and Aquatic Ecology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan

Abstract

Skin mucus with strong mechanisms can trap and collect pathogens before contact with the skin. The liver is a place of accumulation and purification of toxins and environmental pollutants and has different structural and functional complications in the face of pollutants. Fish mucus was collected from the epidermis of fish and the activity of alkaline phosphatase enzyme and the amount of soluble protein were measured. A part of liver tissue was prepared and prepared by classical histological method and stained by hematoxylin-eosin method. Then, the prepared slides were prepared using imaging system attached to a microscope and tissue type was examined and the type and severity of lesions were examined. Experimental treatments had no significant effect on the amount of mucus alkaline phosphatase (P> 0.05) but had a significant effect on the amount of mucus soluble protein (P <0.05). The amount of alkaline phosphatase and mucus soluble protein due to treatments fed nano-plastic toxin also increased with increasing concentration. Experimental treatments also had a significant effect on serum ALT and AST (P <0.05) but had no significant effect on serum ALP (P> 0.05), so that ALT and ALP due to treatments fed with The nanoplastic toxin also increased with increasing concentration and the amount of AST also decreased. Nanoplastic toxin caused complications such as necrosis, watery swelling, cloudy swelling, fattening, macrophage accumulation, lateralization of the nucleus, bleeding, and thinning of the sinusoid in the liver. So that swelling had caused the most complication.

Keywords

References

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Journal of Utilization and Cultivation of Aquatics
Volume 10, Issue 4
March 2022
Pages 15-26

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