The effect of sub-lethal concentrations of silver nitrate (AgNO3) on some biochemical indices and P450 gene expression of common carp (Cyprinus carpio)

Document Type : scientific research article

Authors

1 M.Sc. Graduate of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Corresponding Author, Associate Prof., Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

3 . Associate Prof., Dept. of Fisheries and Aquatic Ecology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

4 Associate Prof., Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

5 Ph.D. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

The purpose of this study was to investigate the effect of sub-lethal concentrations of silver nitrate (AgNO3) on some biochemical indices and P450 gene expression of common carp. For this purpose, Common carp with a weighing average of 7 ±0.33 g were divided into 3 treatments (T1: 0.23, T2: 0.68 and T3: 1.13) and 1 control group, each containing 3 replicates, exposed to the effective concentrations of 0.23, 0/68 and 1/13 ppb for 14 day. At the end of the study, blood, gills and liver samples were collected for evaluate of some biochemical indices (total protein, Immunoglobulin, albumin) and P450 gene expression. The results showed that P450 gene expression in the gills of treatment 3 was significantly increased compared to the control group (P <0.05). The final results of this study in connection with exposure to silver nitrate toxin showed that with increasing duration and concentration of silver nitrate toxin has significant effects on biochemical indices of common carp and in most experiments reduces these indices. Total protein in the 2nd and 3rd groups significantly decreased compared to the control group (P <0.05). Total immunoglobulin levels increased with increasing concentrations of toxin, but this increase was not significant (P> 0.05). Albumin level in treatment 3 was significantly lower than control group (P <0.05) but in other treatments no significant difference was observed with the control group. Also, the expression of P450 gene under the influence of toxin in the liver and gills on day 14 showed the highest expression. Therefore, it can be concluded that silver nitrate can have destructive effects on common carp, which is one of the most important resource of the Caspian Sea.

Keywords

References

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Journal of Utilization and Cultivation of Aquatics
Volume 10, Issue 4
March 2022
Pages 1-13

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