Effects of safflower (Carthamus tinctorius) extract administration on hematology parameters in two different solvent (ethanol and normal saline) via intra peritoneal injection in rainbow trout (Oncorhynchus mykiss)

Document Type : scientific research article

Authors

1 Department of Fisheris, Facultiy of Natural Resources and Marin Sciences, Tarbiat Modares University, Noor, Iran.

2 Inland Waters Aquatic Stocks Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Gorgan, Iran

Abstract

In the present study the effects of safflower (Carthamus tinctorius) extract administration on hematology parameters in two different solvent (ethanol and normal saline) via intra peritoneal injection method have been studied in rainbow trout (Oncorhynchus mykiss). In this regard, 3 treatment groups, one positive control group (injected just with solvent) and one negative control (with no injection) were considered for each solvent. Treatment groups for each sulvent injected with different levels of sufflower extract including 50, 100 and 200 mg/kbw. According to the results, in fish that injected with ethanol solvent showed 15.6 – 20 % mortality, no mortality was recorded in fish that injected with normal saline as solvent. According to the haematological study white blood cell count (WBC) were calculated significantlly lower in all treatment groups that received sufflower extract at 3th, 7th and 10th days post injection compre to controls. Andso in treatment groups that recieved sufflower extract in normal saline as solvent MCV and MCH indices significantly decreased compare with controls (p ≤ 0.05), these indices didn’t changed in treatments that injected with normal saline (p ˃ 0.05).

Keywords

Main Subjects

References

1.Altundag, M.S., Tiril, S.U., and Ozdemir, A. 2014. Effects of safflower oil supplementation in diet on growth performance and body fatty acid composition of turbot (Psetta maxima). Aquaculture international. 22: 2. 597-605.
2.Barton, B.A. 2002. Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and comparative biology. 42: 3. 517-525.
3.Blaxhall, P.C. 1972. The haematological assessment of the health of freshwater fish. J. Fish Biol. 4: 4. 593-604.
4.Dacie, J.V., and Lewis, S.M. 1984. Practical Haematology. 6th Edition. Churchill Livingstone Publishers. Singapore. 453p.
5.Dadras, H., Hayatbakhsh, M.R., Shelton, W.L., and Golpour, A. 2016. Effects of dietary administration of Rose hip and Safflower on growth performance, haematological, biochemical parameters and innate immune response of Beluga, Huso huso (Linnaeus, 1758). Fish & shellfish immunology. 59: 109-114.
6.Dehghani Ghomeshani, M., Mazandarani, M., Sudagar, M., and Hosseini, S.M. 2017. Haematological study of safflower (Carthamus tinctorius) extract fed common carp (Cyprinus carpio) fingerlings exposed to sub lethal salinity stress. Breed. Aquacul. Sci. J. 5: 4. 55-69.
7.Dernekbaşı, S., Kerim, M., and Alagil, F. 2015. Effect of Dietary Safflower and Canola Oil on Growth Performance, Body, and Fatty Acid Composition of Rainbow Trout (Oncorhynchus mykiss). J. Aqua. Food Prod. Technol. 24: 2. 131-142.
8.FAO. 2014. State of World Fisheries and Aquaculture. FAO, Rome.
9.Harikrishnan, R., Balasundaram, C., Kim, M.C., Kim, J.S., Han, Y.J., and Heo, M.S. 2009. Innate immune response and disease resistance in Carassius auratus by triherbal solvent extracts. Fish and shellfish immunology. 27: 3. 508-515.
10.Kiron, V. 2012. Fish immune system and its nutritional modulation for preventive health care. Animal Feed Science Technology. 173: 111-133.
11.Lim, C., Ako, H., Brown, C.L.,and Hahn, K. 1997. Growth response and fatty acid composition of juvenile Penaeus vannamei fed differentsources of dietary lipid. Aquaculture. 151: 1-4. 143-153.
12.Louei Monfared, A., and Salat, A.P. 2012. The effects of Carthamus tinctorius L. on placental histomorphology and survival of the neonates in mice. Avicenna J. Phytomed. 2: 3. 146-152.
13.Lu, Z.W., Liu, F., Hu, J., Bian, D.,and Li, F.G. 1991. Suppressive effects of safflower yellow on immune functions. Acta pharmacologica Sinica, 12: 6. 537-542.
14.Malekian, M., and Hasan abadi, A. 2011. Effect of different levels of safflower seed on the performance of broiler chickens from 21 to 42 days. Iran. J. Anim. Sci. Res. 3: 1. 1-8.
15.Villa, C., Costa, J., Oliveira, M.B.P., and Mafra, I. 2017. Novel quantitative real-time PCR approach to determine safflower (Carthamus tinctorius) adulteration in saffron (Crocus sativus). Food Chemistry. 229: 680-687.
16.Zhou, X., Tang, L., Xu, Y., Zhou, G., and Wang, Z. 2014. Towards a better understanding of medicinal uses of Carthamus tinctorius L. in traditional Chinese medicine: A phytochemical and pharmacological review. J. Ethnopharmacol. 151: 1. 27-43.
Journal of Utilization and Cultivation of Aquatics
Volume 8, Issue 2
July 2019
Pages 61-72

Files

Share

How to cite

Statistics