The effects Pollution of Lead Heavy Metals contamination on the Antioxidant Activity of microalgae Spirulina Platensis

Document Type : scientific research article

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Abstract

Heavy metals are the most important environmental pollution, which are resistant in nature. Increasing levels of heavy metals in aquatic environments has biochemical and physiological negative effects on microalgae. Microalgae increase the antioxidant content in order to protect cells from stress conditions. Probably, heavy metals cause to creating oxidative stress by producing free radical stress. This various forms reacts with lipids and will cause of lipid peroxidation, membrane damage and inactivation of enzymes; so cell life will be compromise. In this study, effect of different concentrations of Lead (5, 10, 30, 50 and 100 micrograms per liter) on the antioxidant activity, Phenolic and flavonoid content of Spirulina Platensis microalgae methanol extract was studied in a period of 8 days. High performance of Phenolic compounds, especially Phenolic acids and Flavonoids in creating ant oxidative properties is important. Estimation of antioxidant activity according to DPPH method, indicated that the compounds levels of Phenolic and Flavonoid have been increased by the microalgae in order to coping with effects of heavy metals oxidative; and the highest antioxidant activity was seen at the last days of estimation. Also, assessing the special rate of growth showed that the highest rate is belong to the control group. In addition, by increasing the Lead concentration, the growth rate had more decreased.

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Ames, B.N., Shegenaga, M.K., and Hagen, T.M. 1993. Oxidative, Antioxidants and the degenerative diseases of aging. Prodceedings of the National Academy of Sciences of the United States of America. 20(17): 7915-7922.
Arunakumara, K., Zhang, X., and Song, X. 2008. Bioaccumulation of Pb2+ and its effects on growth, morphology and pigment contents of Spirulina (Arthrospira) platensis. J. of Ocean University of China., 7(4): 397-403.
Barcelo, J., and Poschenrieder, C. 2004. Structural and ultrastructural changes in heavy metal exposed plants. In: Prasad MNV (eds) Heavy metal stress in plants, 3rd edn. Springer, Berlin; 223-248.
Blokhina, O., Virolainen, E., and Fangerstedt, K.V. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot., 91(2): 179- 94.
Chang, C., Yang, M., Wen, H., and Chern, J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. of Food and Drug Analysis 10(1): 178-182.
Davies, A.G. 1978. Pollution Studies with marine Plankton; Part 2. Heavy Metals. Advances in Marin Biology. 
15: 381-508.
Hanaa, H., Baky, A.E., Baz, F.K.El., and Gamal, S. 2007. Enhancement of Antioxidant Production in Spirulina Platensis under Oxidative Stress. Eurasian J. of Scientific Research. 2(2): 170- 179.
Hossein zade, KH., Ganjian Khenari, A., and Jafari, M. 2013. Effects of Water Enrichment on microalgae Spirulina Platensis growth Parameters in the Southern Caspian Sea. J. Biochemistry Aquatich. 1(1): 71- 81. (In Persian)
Karadeniz, A., and Cemek, M. 2006. Protective Effect of Spirulina Platensis Against Lead Toxication in Rats. J. of Animal and Veterinary Advances. 5(1): 1113- 1116.
 Mashayekhi, K., and Atashi, S. 2014. The analyzing methods in Plant Physiology. 1 th edn.  Vajhegane Sirang, Gorgan, Iran. 317p. (In Persian)
 Plasket, D., and Potter, I. 1979. Heavy metal concentrations in the muscle tissue of 12 species of teleosts from corborn sound, Western Australia. Australia J. of Marin and freshwater research. 30(5). 607p.
 Retnaningsih Soeprobowati, T., Hariyati, R. 2014. Phycoremediation of Pb+2, Cd+2, Cu+2 and Cr+2 by Spirulina Platensis (Gomont Geitler). American Journal of Bio Science. 2(4): 165-170.
Victory, KJ. 2008. Isolation and Characterization of Antimicrobial Compounds Synthesized by Microcystis SP. Phd Thesis. School of Chemical Engineering, Faculty of Engineering Computer and Mathematical Sciences. 36-38.
 Wicher, A.M., and Gantt, L.K. 1994. Contaminant assessment of fish rangia clams and sediments in the lower Pamlico river, North Carolina, U.S fish and wildfish service Ecological services 3(5): 213-220.