Assessment of Protein Synthesis and Accumulation Rate in Cultivated Spirulina (Spirulina platensis) Microalgae in Dairy Industry Wastewater

Articles in Press

Document Type : scientific research article

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1 Ph.D student

2 science committee

Abstract

Microalgae, photosynthetic microorganisms, are highly valued due to their protein, lipid, and carbohydrate compounds.The main objective of this study was to investigate the impact of wastewater dilution ratio on protein accumulation in Spirulina microalgae. By altering the wastewater dilution ratio in the cultivation medium, the effect on protein synthesis and accumulation processes in Spirulina was evaluated. In this study, the following methods were employed for cultivating the microalgae S. platensis: preparation and sterilization of the culture medium using UV lamp and drying of the equipment at 180 degrees Celsius. The cultivation conditions included a temperature range of 22 to 25 OC, light intensity of 350±3500 lux, and a light-dark cycle of 12 hours each for duration of 14 days in 500 mL Erlenmeyer flasks. The study consisted of 4 treatments and a control group, where the treatments involved different levels of wastewater utilization, and the control group utilized only the culture medium without wastewater. Subsequently, the amino acid profile of the microalgal treatment with the highest protein content was determined and prepared for the next phase of analysis. The results indicated that the 100% treatment had the highest levels of nitrates, phosphates, ammonia, biomass, and protein, while the 25% treatment showed the lowest levels. Additionally. In the analysis of amino acids, different levels were observed among the different treatments,. Overall, the results of this study demonstrated that the use of wastewater as a water source in the cultivation of Spirulina platensis microalgae can lead to significant improvements in growth and the production of nutrients and biochemical compounds in these algae.

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Journal of Utilization and Cultivation of Aquatics

Articles in Press, Accepted Manuscript
Available Online from 03 June 2025

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