Evaluation the cytotoxicity effects of protein hydrolysates prepared from Greater lizardfish (Saurida tumbil (Bloch, 1975)) on mouse breast cancer cell lines (4T1)

Today, the attention of researchers is directed to natural sources to discover new anticancer drugs, so that more than 60% of existing anticancer drugs are of natural origin. Bioactive peptides from aquatic protein sources with multiple biological activities, including anticancer, are promising in this field. The present study was conducted with the aim of evaluating the cytotoxicity of hydrolyzed protein of Greater lizardfish on breast cancer cells. Greater lizardfish meat was hydrolyzed with enzymatic hydrolysis with alcalase and papain enzymes with ratios of 2 and 4% and during 90 and 180 minutes. Mouse breast cancer cell lines (4T1) were subjected to protein hydrolysate samples (1 mg/ml) and incubated for 48 and 72 hours, and then cytotoxicity was determined by MTT colorimetric test. The samples hydrolyzed with alcalase enzyme showed strong cytotoxicity in 48 hours (14.19 to 74.54%) and 72 hours (95.51 to 96.14%) incubation. The samples hydrolyzed with papain also showed moderate toxicity in 48-hour incubation (5.72 to 26.56%) and strong toxicity in 72-hour incubation (93.27 to 96.26%). The highest cytotoxicity in 48-hour incubation was observed by the sample hydrolyzed with alcalase at a concentration of 4% for 180 minutes (74.54%), but in 72-hour incubation, all samples showed cytotoxicity above 90%. The protein hydrolysate of Greater lizardfish inhibited the growth of breast cancer cells and probably with more research in the future, it can be used in cancer treatment.